##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AclI AcyI AgeI Alw44I
ApaLI AscI AseI AsnI AspI
AviII BamHI BbeI BbrPI BlpI
Bpu1102I BsaHI BsiWI BsmBI Bsp1407I
BspDI BspHI BsrGI BssHII BssSI
BstAPI CelII ClaI CpoI EcoNI
FseI FspI Hin1I HindIII HpaI
KasI MfeI MunI NarI NdeI
NheI NotI NruI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 4648 GTATAC GT^[AC][GT]AC
AhdI 1095 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 5218 CCTCAGG CC^T[ACGT]AGG
Aor51HI 156 AGCGCT AGC^GCT
ApaI 298 GGGCCC GGGCC^C
AspEI 1095 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 3190 CCTCAGC CCTCAGC(-5/-2)
BcgI 1910 CGATGTGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3557 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 560 CACGTA [CT]AC^GT[GA]
BsiEI 54 CGGCCG CG[GA][CT]^CG
BsmI 4700 GCATTC GAATGC(1/-1)
BspLU11I 3579 ACATGT A^CATGT
BspMI 1259 GCAGGT ACCTGC(4/8)
BsrBI 1197 CCGCTC CCGCTC(-3/-3)
BsrDI 2197 CATTGC GCAATG(2/0)
Bst1107I 4648 GTATAC GTA^TAC
BstBI 1303 TTCGAA TT^CGAA
BstEII 733 GGTCACC G^GT[ACGT]ACC
BstPI 733 GGTCACC G^GT[ACGT]ACC
BstZ17I 4648 GTATAC GTA^TAC
Bsu36I 5218 CCTCAGG CC^T[ACGT]AGG
DraIII 3690 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 5182 GACTGTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 54 CGGCCG C^GGCCG
Eam1105I 1095 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 54 CGGCCG C^GGCCG
Eco47III 156 AGCGCT AGC^GCT
Eco52I 54 CGGCCG C^GGCCG
Eco81I 5218 CCTCAGG CC^T[ACGT]AGG
EcoO65I 733 GGTCACC G^GT[ACGT]ACC
EcoRV 1773 GATATC GAT^ATC
EcoT22I 1000 ATGCAT ATGCA^T
MluI 458 ACGCGT A^CGCGT
NaeI 354 GCCGGC GCC^GGC
NcoI 4005 CCATGG C^CATGG
NgoMIV 354 GCCGGC G^CCGGC
NsiI 1000 ATGCAT ATGCA^T
NspI 3579 ACATGT [GA]CATG^[CT]
NspV 1303 TTCGAA TT^CGAA
PaeR7I 1851 CTCGAG C^TCGAG
PpuMI 680 AGGTCCC [GA]G^G[AT]CC[CT]
SapI 2160 GAAGAGC GCTCTTC(1/4)
ScaI 3246 AGTACT AGT^ACT
SfuI 1303 TTCGAA TT^CGAA
SpeI 4627 ACTAGT A^CTAGT
XhoI 1851 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 1825 GGTACC G^GTACC
4020 GGTACC
AccIII 278 TCCGGA T^CCGGA
545 TCCGGA
AflII 3341 CTTAAG C^TTAAG
4810 CTTAAG
Asp718I 1825 GGTACC G^GTACC
4020 GGTACC
AvaI 295 CTCGGG C^[CT]CG[GA]G
1851 CTCGAG
AvrII 840 CCTAGG C^CTAGG
2363 CCTAGG
BciVI 4009 GGATAC GTATCC(6/5)
2076 GTATCC
BclI 336 TGATCA T^GATCA
2104 TGATCA
BfrI 3341 CTTAAG C^TTAAG
4810 CTTAAG
BlnI 840 CCTAGG C^CTAGG
2363 CCTAGG
BsaBI 1430 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1662 GATATGAATC
BseAI 278 TCCGGA T^CCGGA
545 TCCGGA
BseRI 1800 GAGGAG GAGGAG(10/8)
2758 GAGGAG
BsoBI 295 CTCGGG C^[CT]CG[GA]G
1851 CTCGAG
BspEI 278 TCCGGA T^CCGGA
545 TCCGGA
BsrFI 354 GCCGGC [GA]^CCGG[CT]
5357 GCCGGT
BstXI 738 CCAGCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3390 CCATTTGAATGG
Cfr10I 354 GCCGGC [GA]^CCGG[CT]
5357 GCCGGT
EcoRI 1125 GAATTC G^AATTC
5667 GAATTC
FbaI 336 TGATCA T^GATCA
2104 TGATCA
HaeII 156 AGCGCT [GA]GCGC^[CT]
3068 GGCGCT
HgaI 457 GACGC GACGC(5/10)
1588 GCGTC
HincII 1466 GTTGAC GT[CT]^[GA]AC
1893 GTCAAC
HindII 1466 GTTGAC GT[CT]^[GA]AC
1893 GTCAAC
KpnI 1825 GGTACC GGTAC^C
4020 GGTACC
KspI 51 CCGCGG CCGC^GG
186 CCGCGG
MamI 1430 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1662 GATATGAATC
MroI 278 TCCGGA T^CCGGA
545 TCCGGA
PflMI 809 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2318 CCAGGACGTGG
SacI 993 GAGCTC GAGCT^C
3290 GAGCTC
SacII 51 CCGCGG CCGC^GG
186 CCGCGG
SwaI 2911 ATTTAAAT ATTT^AAAT
5598 ATTTAAAT
TaqI 1304 TCGA T^CGA
1852 TCGA
TthHB8I 1304 TCGA T^CGA
1852 TCGA
Van91I 809 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2318 CCAGGACGTGG
XbaI 3822 TCTAGA T^CTAGA
5175 TCTAGA
XcmI 2318 CCAGGACGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5505 CCAGAACACATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI