##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AscI AseI
AsnI Asp700I Asp718I AspEI BamHI
BcgI BciVI BclI BfrI BglII
BsaWI BseAI BsiWI BsmI BspDI
BspEI BspHI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DrdI Eam1105I EcoO65I EcoRV EcoT22I
FbaI HincII HindII HpaI KpnI
KspI MluI MroI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AocI 5071 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2465 AGCGCT AGC^GCT
AvrII 556 CCTAGG C^CTAGG
BlnI 556 CCTAGG C^CTAGG
BlpI 1600 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1600 GCTGAGC GC^T[ACGT]AGC
BsaBI 2157 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 662 CGGCCG CG[GA][CT]^CG
Bsp1407I 3737 TGTACA T^GTACA
BsrDI 4358 CATTGC GCAATG(2/0)
BsrGI 3737 TGTACA T^GTACA
BstAPI 469 GCAGATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 2360 AGATCC [GA]^GATC[CT]
Bsu36I 5071 CCTGAGG CC^T[ACGT]AGG
CelII 1600 GCTGAGC GC^T[ACGT]AGC
EagI 662 CGGCCG C^GGCCG
EclXI 662 CGGCCG C^GGCCG
Eco47III 2465 AGCGCT AGC^GCT
Eco52I 662 CGGCCG C^GGCCG
Eco81I 5071 CCTGAGG CC^T[ACGT]AGG
FseI 1059 GGCCGGCC GGCCGG^CC
HindIII 2410 AAGCTT A^AGCTT
MamI 2157 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 2360 AGATCC [GA]^GATC[CT]
NdeI 174 CATATG CA^TATG
PmeI 2929 GTTTAAAC GTTT^AAAC
PshAI 4168 GACGGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2615 GAAGAGC GCTCTTC(1/4)
SexAI 874 ACCAGGT A^CC[AT]GGT
SspBI 3737 TGTACA T^GTACA
XhoII 2360 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 1560 GATCC GGATC(4/5)
2361 GATCC
AspI 1344 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5544 GACAGGGTC
AviII 4619 TGCGCA TGC^GCA
4713 TGCGCA
BbsI 1913 GAAGAC GAAGAC(2/6)
4427 GTCTTC
BpuAI 1913 GAAGAC GAAGAC(2/6)
4427 GTCTTC
BssSI 4053 CACGAG CACGAG(-5/-1)
3220 CTCGTG
DraIII 873 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2445 CACTGTGTG
EarI 5662 CTCTTC CTCTTC(1/4)
2615 GAAGAG
EcoRI 1755 GAATTC G^AATTC
2690 GAATTC
FspI 4619 TGCGCA TGC^GCA
4713 TGCGCA
Ksp632I 5662 CTCTTC CTCTTC(1/4)
2615 GAAGAG
MfeI 740 CAATTG C^AATTG
3184 CAATTG
MunI 740 CAATTG C^AATTG
3184 CAATTG
PflFI 1344 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5544 GACAGGGTC
SfiI 663 GGCCGCCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2300 GGCCTGTAGGGCC
SmaI 1413 CCCGGG CCC^GGG
1746 CCCGGG
SmlI 2147 CTTGAG C^T[CT][GA]AG
5594 CTCAAG
TaqI 1123 TCGA T^CGA
1542 TCGA
Tth111I 1344 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5544 GACAGGGTC
TthHB8I 1123 TCGA T^CGA
1542 TCGA
XbaI 952 TCTAGA T^CTAGA
2326 TCTAGA
XmaCI 1413 CCCGGG C^CCGGG
1746 CCCGGG
XmaI 1413 CCCGGG C^CCGGG
1746 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwNI ApaI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbeI BbrPI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI