##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AgeI Alw44I Aor51HI ApaLI AscI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BglI BlnI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoO65I EcoT22I FseI
FspI HapII HgaI Hin1I HpaII
KasI KspI MfeI MluI MroI
MspI MunI NaeI NarI NciI
NgoMIV NotI NruI NsiI NspV
PflFI PinAI PmaCI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SphI Sse8387I
SspBI StuI SwaI Tth111I XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 3882 GGTACC G^GTACC
AhdI 4709 GACTGTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 438 GGGCCC GGGCC^C
Asp700I 1634 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3882 GGTACC G^GTACC
AspEI 4709 GACTGTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 4016 CTCGAG C^[CT]CG[GA]G
BalI 428 TGGCCA TGG^CCA
BanI 3882 GGTACC G^G[CT][GA]CC
BglII 1293 AGATCT A^GATCT
BsaAI 2217 TACGTA [CT]AC^GT[GA]
BseRI 4289 GAGGAG GAGGAG(10/8)
BsoBI 4016 CTCGAG C^[CT]CG[GA]G
BssSI 4163 CACGAG CACGAG(-5/-1)
BstXI 4537 CCATTTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 4223 GACAGCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1471 CCATGG C^C[GA][CT]GG
EaeI 428 TGGCCA [CT]^GGCC[GA]
Eam1105I 4709 GACTGTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 1672 GCGGG CCCGC(4/6)
HpaI 3450 GTTAAC GTT^AAC
KpnI 3882 GGTACC GGTAC^C
MluNI 428 TGGCCA TGG^CCA
MscI 428 TGGCCA TGG^CCA
NcoI 1471 CCATGG C^CATGG
NdeI 1829 CATATG CA^TATG
PacI 1019 TTAATTAA TTAAT^TAA
PaeR7I 4016 CTCGAG C^TCGAG
PmeI 2985 GTTTAAAC GTTT^AAAC
SnaBI 2217 TACGTA TAC^GTA
SpeI 4972 ACTAGT A^CTAGT
XhoI 4016 CTCGAG C^TCGAG
XmnI 1634 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 375 CGCG CG^CG
2937 CGCG
AocI 242 CCTAAGG CC^T[ACGT]AGG
610 CCTGAGG
BbsI 2482 GAAGAC GAAGAC(2/6)
4936 GAAGAC
BclI 2340 TGATCA T^GATCA
2960 TGATCA
BlpI 139 GCTGAGC GC^T[ACGT]AGC
1090 GCTAAGC
Bpu1102I 139 GCTGAGC GC^T[ACGT]AGC
1090 GCTAAGC
BpuAI 2482 GAAGAC GAAGAC(2/6)
4936 GAAGAC
BspHI 2555 TCATGA T^CATGA
2957 TCATGA
BspMI 810 ACCTGC ACCTGC(4/8)
3391 ACCTGC
BstAPI 455 GCAATATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
886 GCAGACCTTGC
BstUI 375 CGCG CG^CG
2937 CGCG
Bsu36I 242 CCTAAGG CC^T[ACGT]AGG
610 CCTGAGG
CelII 139 GCTGAGC GC^T[ACGT]AGC
1090 GCTAAGC
DraIII 566 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3252 CACACAGTG
Eco81I 242 CCTAAGG CC^T[ACGT]AGG
610 CCTGAGG
EcoNI 4638 CCTGTAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5482 CCTAATACAGG
EcoRI 2559 GAATTC G^AATTC
5317 GAATTC
FbaI 2340 TGATCA T^GATCA
2960 TGATCA
HaeII 1355 AGCGCC [GA]GCGC^[CT]
4104 AGCGCC
HindIII 8 AAGCTT A^AGCTT
123 AAGCTT
MaeII 901 ACGT A^CGT
2218 ACGT
MvnI 375 CGCG CG^CG
2937 CGCG
NheI 3052 GCTAGC G^CTAGC
4487 GCTAGC
PflMI 3251 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4048 CCAGACCCTGG
PpuMI 1885 AGGACCC [GA]G^G[AT]CC[CT]
3388 AGGACCT
PstI 884 CTGCAG CTGCA^G
1608 CTGCAG
RcaI 2555 TCATGA T^CATGA
2957 TCATGA
Van91I 3251 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4048 CCAGACCCTGG
XbaI 1459 TCTAGA T^CTAGA
4958 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI AseI
AsnI AspHI AvaII BanII BbvI
BfaI BfrI BmyI BpmI BsaBI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspLU11I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII EcoRV EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII