##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AgeI        Alw44I      Aor51HI     ApaLI       AscI        
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BcnI        
BglI        BlnI        BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BsrBI       BsrFI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Cfr10I      ClaI        CpoI        EagI        EclXI       
Eco47III    Eco52I      EcoO65I     EcoT22I     FseI        
FspI        HapII       HgaI        Hin1I       HpaII       
KasI        KspI        MfeI        MluI        MroI        
MspI        MunI        NaeI        NarI        NciI        
NgoMIV      NotI        NruI        NsiI        NspV        
PflFI       PinAI       PmaCI       PmlI        PshAI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SphI        Sse8387I    
SspBI       StuI        SwaI        Tth111I     XcmI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

Acc65I       3882  GGTACC               G^GTACC
AhdI         4709  GACTGTTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI          438  GGGCCC               GGGCC^C
Asp700I      1634  GAACACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      3882  GGTACC               G^GTACC
AspEI        4709  GACTGTTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         4016  CTCGAG               C^[CT]CG[GA]G
BalI          428  TGGCCA               TGG^CCA
BanI         3882  GGTACC               G^G[CT][GA]CC
BglII        1293  AGATCT               A^GATCT
BsaAI        2217  TACGTA               [CT]AC^GT[GA]
BseRI        4289  GAGGAG               GAGGAG(10/8)
BsoBI        4016  CTCGAG               C^[CT]CG[GA]G
BssSI        4163  CACGAG               CACGAG(-5/-1)
BstXI        4537  CCATTTTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI         4223  GACAGCTTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1471  CCATGG               C^C[GA][CT]GG
EaeI          428  TGGCCA               [CT]^GGCC[GA]
Eam1105I     4709  GACTGTTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI         1672  GCGGG                CCCGC(4/6)
HpaI         3450  GTTAAC               GTT^AAC
KpnI         3882  GGTACC               GGTAC^C
MluNI         428  TGGCCA               TGG^CCA
MscI          428  TGGCCA               TGG^CCA
NcoI         1471  CCATGG               C^CATGG
NdeI         1829  CATATG               CA^TATG
PacI         1019  TTAATTAA             TTAAT^TAA
PaeR7I       4016  CTCGAG               C^TCGAG
PmeI         2985  GTTTAAAC             GTTT^AAAC
SnaBI        2217  TACGTA               TAC^GTA
SpeI         4972  ACTAGT               A^CTAGT
XhoI         4016  CTCGAG               C^TCGAG
XmnI         1634  GAACACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII         375  CGCG                 CG^CG
             2937  CGCG                
AocI          242  CCTAAGG              CC^T[ACGT]AGG
              610  CCTGAGG             
BbsI         2482  GAAGAC               GAAGAC(2/6)
             4936  GAAGAC              
BclI         2340  TGATCA               T^GATCA
             2960  TGATCA              
BlpI          139  GCTGAGC              GC^T[ACGT]AGC
             1090  GCTAAGC             
Bpu1102I      139  GCTGAGC              GC^T[ACGT]AGC
             1090  GCTAAGC             
BpuAI        2482  GAAGAC               GAAGAC(2/6)
             4936  GAAGAC              
BspHI        2555  TCATGA               T^CATGA
             2957  TCATGA              
BspMI         810  ACCTGC               ACCTGC(4/8)
             3391  ACCTGC              
BstAPI        455  GCAATATATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              886  GCAGACCTTGC         
BstUI         375  CGCG                 CG^CG
             2937  CGCG                
Bsu36I        242  CCTAAGG              CC^T[ACGT]AGG
              610  CCTGAGG             
CelII         139  GCTGAGC              GC^T[ACGT]AGC
             1090  GCTAAGC             
DraIII        566  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3252  CACACAGTG           
Eco81I        242  CCTAAGG              CC^T[ACGT]AGG
              610  CCTGAGG             
EcoNI        4638  CCTGTAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5482  CCTAATACAGG         
EcoRI        2559  GAATTC               G^AATTC
             5317  GAATTC              
FbaI         2340  TGATCA               T^GATCA
             2960  TGATCA              
HaeII        1355  AGCGCC               [GA]GCGC^[CT]
             4104  AGCGCC              
HindIII         8  AAGCTT               A^AGCTT
              123  AAGCTT              
MaeII         901  ACGT                 A^CGT
             2218  ACGT                
MvnI          375  CGCG                 CG^CG
             2937  CGCG                
NheI         3052  GCTAGC               G^CTAGC
             4487  GCTAGC              
PflMI        3251  CCACACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4048  CCAGACCCTGG         
PpuMI        1885  AGGACCC              [GA]G^G[AT]CC[CT]
             3388  AGGACCT             
PstI          884  CTGCAG               CTGCA^G
             1608  CTGCAG              
RcaI         2555  TCATGA               T^CATGA
             2957  TCATGA              
Van91I       3251  CCACACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4048  CCAGACCCTGG         
XbaI         1459  TCTAGA               T^CTAGA
             4958  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AciI        AcsI        AfaI        AflII       AflIII      
AluI        AlwI        AlwNI       ApoI        AseI        
AsnI        AspHI       AvaII       BanII       BbvI        
BfaI        BfrI        BmyI        BpmI        BsaBI       
BsaI        BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BspLU11I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       EarI        
EcoO109I    EcoRII      EcoRV       EcoT14I     Fnu4HI      
FokI        HaeIII      HhaI        HinP1I      HincII      
HindII      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MamI        MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PshBI       PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII