##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
AscI Asp718I AviII BbeI BsiEI
BsiWI BspDI BsrFI BssHII BssSI
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI DraIII EagI EclXI Eco47III
Eco52I FseI FspI HaeII KasI
KpnI KspI MluI NaeI NarI
NgoMIV NotI NspV PacI PaeR7I
PinAI PmeI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 4111 GTAGAC GT^[AC][GT]AC
AccII 5637 CGCG CG^CG
AccIII 3371 TCCGGA T^CCGGA
AcyI 5312 GGCGTC G[GA]^CG[CT]C
AhdI 4161 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2297 GGGCCC GGGCC^C
AspEI 4161 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2813 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3832 CCCGGG C^[CT]CG[GA]G
AvrII 4340 CCTAGG C^CTAGG
BamHI 3374 GGATCC G^GATCC
BbrPI 1160 CACGTG CAC^GTG
BcgI 838 CGAACAGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 4936 GCCTGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4340 CCTAGG C^CTAGG
BsaHI 5312 GGCGTC G[GA]^CG[CT]C
BsaWI 3371 TCCGGA [AT]^CCGG[AT]
BseAI 3371 TCCGGA T^CCGGA
BsmBI 3739 CGTCTC CGTCTC(1/5)
BsoBI 3832 CCCGGG C^[CT]CG[GA]G
BspEI 3371 TCCGGA T^CCGGA
BspHI 272 TCATGA T^CATGA
BsrBI 49 CCGCTC CCGCTC(-3/-3)
BstAPI 3840 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 5637 CGCG CG^CG
CfoI 1689 GCGC GCG^C
DsaI 748 CCATGG C^C[GA][CT]GG
Eam1105I 4161 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 709 CCTTTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 5444 GATATC GAT^ATC
HhaI 1689 GCGC GCG^C
Hin1I 5312 GGCGTC G[GA]^CG[CT]C
HinP1I 1689 GCGC G^CGC
MfeI 3408 CAATTG C^AATTG
MroI 3371 TCCGGA T^CCGGA
MunI 3408 CAATTG C^AATTG
MvnI 5637 CGCG CG^CG
NcoI 748 CCATGG C^CATGG
NheI 1365 GCTAGC G^CTAGC
NruI 5636 TCGCGA TCG^CGA
PflFI 2813 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1073 CCACACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1160 CACGTG CAC^GTG
PmlI 1160 CACGTG CAC^GTG
PshAI 4114 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 272 TCATGA T^CATGA
SapI 295 GAAGAGC GCTCTTC(1/4)
SmaI 3832 CCCGGG CCC^GGG
SnaBI 314 TACGTA TAC^GTA
TaqI 3707 TCGA T^CGA
Tth111I 2813 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 3707 TCGA T^CGA
Van91I 1073 CCACACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 3832 CCCGGG C^CCGGG
XmaI 3832 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 1157 GTGCAC G^TGCAC
3777 GTGCAC
ApaLI 1157 GTGCAC G^TGCAC
3777 GTGCAC
AseI 69 ATTAAT AT^TAAT
2756 ATTAAT
AsnI 69 ATTAAT AT^TAAT
2756 ATTAAT
BciVI 311 GGATAC GTATCC(6/5)
2636 GGATAC
BglII 540 AGATCT A^GATCT
1149 AGATCT
BsaAI 314 TACGTA [CT]AC^GT[GA]
1160 CACGTG
BsaBI 770 GATAGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
897 GATGGGAATC
Bsp1407I 1395 TGTACA T^GTACA
1999 TGTACA
BsrGI 1395 TGTACA T^GTACA
1999 TGTACA
BstEII 2850 GGTGACC G^GT[ACGT]ACC
4120 GGTCACC
BstPI 2850 GGTGACC G^GT[ACGT]ACC
4120 GGTCACC
DrdI 954 GACATTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4535 GACCTCCCAGTC
EcoO65I 2850 GGTGACC G^GT[ACGT]ACC
4120 GGTCACC
EcoRI 821 GAATTC G^AATTC
3150 GAATTC
FauI 48 CCCGC CCCGC(4/6)
4230 GCGGG
HgaI 4095 GACGC GACGC(5/10)
5313 GCGTC
HindIII 1010 AAGCTT A^AGCTT
4075 AAGCTT
MamI 770 GATAGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
897 GATGGGAATC
NdeI 660 CATATG CA^TATG
1664 CATATG
PshBI 69 ATTAAT AT^TAAT
2756 ATTAAT
PvuII 679 CAGCTG CAG^CTG
4208 CAGCTG
SphI 27 GCATGC GCATG^C
4898 GCATGC
SspBI 1395 TGTACA T^GTACA
1999 TGTACA
XbaI 640 TCTAGA T^CTAGA
5630 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BfrI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspLU11I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII Cfr13I DdeI DpnII DraI
DraII EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I EcoT22I FbaI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SpeI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII XmnI