##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
ApaLI AscI Asp700I AviII BbrPI
BcgI BseAI BsiWI BspDI BspEI
BssHII ClaI CpoI EagI EclXI
Eco52I EcoNI EcoRI EcoRV EcoT22I
FseI FspI HpaI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SalI
SbfI SfiI SnaBI SpeI Sse8387I
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 1114 GGTACC G^GTACC
AhdI 2088 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4378 AGCGCT AGC^GCT
ApaI 3388 GGGCCC GGGCC^C
Asp718I 1114 GGTACC G^GTACC
AspEI 2088 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4637 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3928 CCTAGG C^CTAGG
BbeI 1009 GGCGCC GGCGC^C
BbsI 719 GAAGAC GAAGAC(2/6)
BglI 4758 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3928 CCTAGG C^CTAGG
BpuAI 719 GAAGAC GAAGAC(2/6)
BsiEI 908 CGACCG CG[GA][CT]^CG
Bsp1407I 1315 TGTACA T^GTACA
BsrGI 1315 TGTACA T^GTACA
BssSI 3870 CACGAG CACGAG(-5/-1)
BstBI 5036 TTCGAA TT^CGAA
BstEII 18 GGTGACC G^GT[ACGT]ACC
BstPI 18 GGTGACC G^GT[ACGT]ACC
DraIII 591 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1574 GACCCCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2088 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4378 AGCGCT AGC^GCT
EcoO65I 18 GGTGACC G^GT[ACGT]ACC
HincII 4097 GTTGAC GT[CT]^[GA]AC
HindII 4097 GTTGAC GT[CT]^[GA]AC
HindIII 1557 AAGCTT A^AGCTT
KasI 1009 GGCGCC G^GCGCC
KpnI 1114 GGTACC GGTAC^C
NarI 1009 GGCGCC GG^CGCC
NspV 5036 TTCGAA TT^CGAA
PacI 6018 TTAATTAA TTAAT^TAA
PaeR7I 1547 CTCGAG C^TCGAG
PflFI 4637 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1404 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 5208 GAGCTC GAGCT^C
SapI 3613 GAAGAGC GCTCTTC(1/4)
ScaI 1829 AGTACT AGT^ACT
SexAI 1219 ACCAGGT A^CC[AT]GGT
SfoI 1009 GGCGCC GGC^GCC
SfuI 5036 TTCGAA TT^CGAA
SspBI 1315 TGTACA T^GTACA
SspI 5059 AATATT AAT^ATT
SwaI 2806 ATTTAAAT ATTT^AAAT
Tth111I 4637 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1547 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflII 3134 CTTAAG C^TTAAG
4021 CTTAAG
BfrI 3134 CTTAAG C^TTAAG
4021 CTTAAG
BglII 5136 AGATCT A^GATCT
5297 AGATCT
BsaAI 151 TACGTG [CT]AC^GT[GA]
1963 CACGTA
BsaBI 2894 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3463 GATCATTATC
BsaWI 193 ACCGGA [AT]^CCGG[AT]
1438 ACCGGA
BspHI 1902 TCATGA T^CATGA
1998 TCATGA
BsrDI 824 CATTGC GCAATG(2/0)
3729 CATTGC
Bst1107I 3586 GTATAC GTA^TAC
6068 GTATAC
BstAPI 4060 GCACATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5266 GCATGAGCTGC
BstZ17I 3586 GTATAC GTA^TAC
6068 GTATAC
DraI 2807 TTTAAA TTT^AAA
6202 TTTAAA
KspI 310 CCGCGG CCGC^GG
564 CCGCGG
MamI 2894 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3463 GATCATTATC
RcaI 1902 TCATGA T^CATGA
1998 TCATGA
SacII 310 CCGCGG CCGC^GG
564 CCGCGG
SgrAI 609 CGCCGGCG C[GA]^CCGG[CT]G
1005 CACCGGCG
XbaI 2663 TCTAGA T^CTAGA
5024 TCTAGA
XcmI 433 CCAGCATGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1091 CCAGCCCGGCGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI AseI AsnI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbvCI BbvI BciVI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI