##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII Aor51HI AscI AseI AsnI
Asp718I BamHI BcgI BfrI BglII
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eco47III EcoRV EcoT22I HincII HindII
HpaI KpnI KspI MluI MroI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SexAI SfuI SnaBI SpeI
SspBI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AgeI 4508 ACCGGT A^CCGGT
Alw44I 548 GTGCAC G^TGCAC
ApaLI 548 GTGCAC G^TGCAC
AviII 2663 TGCGCA TGC^GCA
BbrPI 3874 CACGTG CAC^GTG
BciVI 734 GGATAC GTATCC(6/5)
BclI 49 TGATCA T^GATCA
BsaAI 3874 CACGTG [CT]AC^GT[GA]
BsaBI 3449 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4508 ACCGGT [AT]^CCGG[AT]
BsrDI 3831 CATTGC GCAATG(2/0)
BssSI 4520 CACGAG CACGAG(-5/-1)
BstEII 1447 GGTGACC G^GT[ACGT]ACC
BstPI 1447 GGTGACC G^GT[ACGT]ACC
EcoO65I 1447 GGTGACC G^GT[ACGT]ACC
EcoRI 5406 GAATTC G^AATTC
FbaI 49 TGATCA T^GATCA
FseI 2805 GGCCGGCC GGCCGG^CC
FspI 2663 TGCGCA TGC^GCA
MamI 3449 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5222 CAATTG C^AATTG
MunI 5222 CAATTG C^AATTG
PinAI 4508 ACCGGT A^CCGGT
PmaCI 3874 CACGTG CAC^GTG
PmlI 3874 CACGTG CAC^GTG
SapI 2551 GCTCTTC GCTCTTC(1/4)
SbfI 8 CCTGCAGG CCTGCA^GG
ScaI 1268 AGTACT AGT^ACT
SgrAI 4507 CACCGGTG C[GA]^CCGG[CT]G
Sse8387I 8 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 4278 GAATTT [GA]^AATT[CT]
5406 GAATTC
AhdI 1795 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3743 GACCCTGTGTC
ApoI 4278 GAATTT [GA]^AATT[CT]
5406 GAATTC
Asp700I 1266 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2370 GAACTGTTTC
AspEI 1795 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3743 GACCCTGTGTC
AspI 1222 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4198 GACATAGTC
AvrII 3491 CCTAGG C^CTAGG
5345 CCTAGG
BlnI 3491 CCTAGG C^CTAGG
5345 CCTAGG
BsmI 2814 GCATTC GAATGC(1/-1)
5374 GCATTC
DraIII 948 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1153 CACCGCGTG
DrdI 1107 GACGCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1432 GACCCGGCTGTC
Eam1105I 1795 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3743 GACCCTGTGTC
PflFI 1222 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4198 GACATAGTC
PshAI 913 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5449 GACTCTTGTC
SacI 2856 GAGCTC GAGCT^C
4848 GAGCTC
SfiI 1164 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2524 GGCCAACCCGGCC
Tth111I 1222 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4198 GACATAGTC
XmnI 1266 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2370 GAACTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI