##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AclI AcsI AflII AflIII AgeI
AhdI ApoI AscI AspEI AviII
BbrPI BcgI BclI BfrI BglII
BsaBI BsiEI BsiWI BsmI Bsp1407I
BspHI BspLU11I BsrDI BsrGI BssHII
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV FbaI FseI
FspI KspI MamI MfeI MluI
MunI NdeI NheI NotI PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacII
SalI SnaBI SspBI SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 3967 GGTACC G^GTACC
AccIII 2503 TCCGGA T^CCGGA
Alw44I 213 GTGCAC G^TGCAC
Aor51HI 1636 AGCGCT AGC^GCT
ApaLI 213 GTGCAC G^TGCAC
Asp700I 3226 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3967 GGTACC G^GTACC
BbeI 1107 GGCGCC GGCGC^C
BseAI 2503 TCCGGA T^CCGGA
BsmBI 3808 GAGACG CGTCTC(1/5)
BspDI 3999 ATCGAT AT^CGAT
BspEI 2503 TCCGGA T^CCGGA
BsrBI 3834 GAGCGG CCGCTC(-3/-3)
BssSI 4030 CTCGTG CACGAG(-5/-1)
Bst1107I 1593 GTATAC GTA^TAC
BstBI 141 TTCGAA TT^CGAA
BstZ17I 1593 GTATAC GTA^TAC
ClaI 3999 ATCGAT AT^CGAT
DrdI 4982 GACAAGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1636 AGCGCT AGC^GCT
EcoNI 5035 CCTAGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1026 ATGCAT ATGCA^T
HgaI 5152 GCGTC GACGC(5/10)
HincII 3153 GTTAAC GT[CT]^[GA]AC
HindII 3153 GTTAAC GT[CT]^[GA]AC
HindIII 2711 AAGCTT A^AGCTT
HpaI 3153 GTTAAC GTT^AAC
KasI 1107 GGCGCC G^GCGCC
KpnI 3967 GGTACC GGTAC^C
MroI 2503 TCCGGA T^CCGGA
NarI 1107 GGCGCC GG^CGCC
NruI 12 TCGCGA TCG^CGA
NsiI 1026 ATGCAT ATGCA^T
NspV 141 TTCGAA TT^CGAA
PshAI 1989 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 4460 CCTGCAGG CCTGCA^GG
ScaI 3268 AGTACT AGT^ACT
SfiI 3792 GGCCAGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1107 GGCGCC GGC^GCC
SfuI 141 TTCGAA TT^CGAA
SgrAI 26 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 4409 ACTAGT A^CTAGT
SphI 4715 GCATGC GCATG^C
Sse8387I 4460 CCTGCAGG CCTGCA^GG
XbaI 6 TCTAGA T^CTAGA
XmnI 3226 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 1630 GACGTC GACGT^C
3327 GACGTC
AocI 2392 CCTCAGG CC^T[ACGT]AGG
4727 CCTCAGG
AseI 4856 ATTAAT AT^TAAT
5191 ATTAAT
AsnI 4856 ATTAAT AT^TAAT
5191 ATTAAT
AspI 112 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4885 GACCTTGTC
AvrII 329 CCTAGG C^CTAGG
5035 CCTAGG
BamHI 2096 GGATCC G^GATCC
3200 GGATCC
BbsI 3540 GAAGAC GAAGAC(2/6)
4979 GAAGAC
BciVI 695 GGATAC GTATCC(6/5)
4820 GTATCC
BlnI 329 CCTAGG C^CTAGG
5035 CCTAGG
BpuAI 3540 GAAGAC GAAGAC(2/6)
4979 GAAGAC
BsaAI 4653 TACGTG [CT]AC^GT[GA]
4817 CACGTA
BsaI 183 GAGACC GGTCTC(1/5)
2463 GAGACC
BsaWI 2503 TCCGGA [AT]^CCGG[AT]
3499 TCCGGT
BstAPI 1554 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3680 GCACCGGCTGC
Bsu36I 2392 CCTCAGG CC^T[ACGT]AGG
4727 CCTCAGG
Eco81I 2392 CCTCAGG CC^T[ACGT]AGG
4727 CCTCAGG
HaeII 1107 GGCGCC [GA]GCGC^[CT]
1636 AGCGCT
NspI 3352 ACATGC [GA]CATG^[CT]
4715 GCATGC
PflFI 112 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4885 GACCTTGTC
PshBI 4856 ATTAAT AT^TAAT
5191 ATTAAT
SacI 3444 GAGCTC GAGCT^C
3983 GAGCTC
SapI 3615 GAAGAGC GCTCTTC(1/4)
1396 GCTCTTC
StuI 3313 AGGCCT AGG^CCT
4132 AGGCCT
TfiI 858 GAATC G^A[AT]TC
4941 GATTC
Tth111I 112 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4885 GACCTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI