##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AccIII AclI AgeI Alw44I ApaI
ApaLI AseI AsnI AspI AvrII
BbrPI BbvCI BcgI BlnI BsaAI
BsaBI BseAI BsiWI Bsp1407I BspEI
BsrGI Bst1107I BstZ17I CpoI DraI
DraIII EcoT22I KspI MamI MluI
MroI NdeI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SfiI SgrAI
SmaI SnaBI SspBI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 2898 GACGTC GACGT^C
AflII 4550 CTTAAG C^TTAAG
AflIII 1004 ACATGT A^C[GA][CT]GT
AhdI 4133 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2766 CCTGAGG CC^T[ACGT]AGG
AscI 501 GGCGCGCC GG^CGCGCC
AspEI 4133 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 156 TGCGCA TGC^GCA
BalI 1226 TGGCCA TGG^CCA
BamHI 1 GGATCC G^GATCC
BbeI 233 GGCGCC GGCGC^C
BciVI 4784 GGATAC GTATCC(6/5)
BfrI 4550 CTTAAG C^TTAAG
BglII 3570 AGATCT A^GATCT
BlpI 2149 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2149 GCTCAGC GC^T[ACGT]AGC
BsmI 4211 GAATGC GAATGC(1/-1)
BspDI 842 ATCGAT AT^CGAT
BspLU11I 1004 ACATGT A^CATGT
BsrFI 284 GCCGGC [GA]^CCGG[CT]
BssSI 2296 CACGAG CACGAG(-5/-1)
BstAPI 894 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2432 TTCGAA TT^CGAA
BstEII 597 GGTCACC G^GT[ACGT]ACC
BstPI 597 GGTCACC G^GT[ACGT]ACC
BstXI 2774 CCAAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2766 CCTGAGG CC^T[ACGT]AGG
CelII 2149 GCTCAGC GC^T[ACGT]AGC
Cfr10I 284 GCCGGC [GA]^CCGG[CT]
ClaI 842 ATCGAT AT^CGAT
DrdI 1003 GACATGTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4133 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2766 CCTGAGG CC^T[ACGT]AGG
EcoO65I 597 GGTCACC G^GT[ACGT]ACC
EcoRI 1818 GAATTC G^AATTC
EcoRV 4576 GATATC GAT^ATC
FseI 283 GGCCGGCC GGCCGG^CC
FspI 156 TGCGCA TGC^GCA
HpaI 5439 GTTAAC GTT^AAC
KasI 233 GGCGCC G^GCGCC
MfeI 5242 CAATTG C^AATTG
MluNI 1226 TGGCCA TGG^CCA
MscI 1226 TGGCCA TGG^CCA
MunI 5242 CAATTG C^AATTG
NaeI 284 GCCGGC GCC^GGC
NarI 233 GGCGCC GG^CGCC
NcoI 1119 CCATGG C^CATGG
NgoMIV 284 GCCGGC G^CCGGC
NheI 1408 GCTAGC G^CTAGC
NspV 2432 TTCGAA TT^CGAA
SalI 1263 GTCGAC G^TCGAC
ScaI 5205 AGTACT AGT^ACT
SexAI 2048 ACCAGGT A^CC[AT]GGT
SfoI 233 GGCGCC GGC^GCC
SfuI 2432 TTCGAA TT^CGAA
SpeI 3741 ACTAGT A^CTAGT
SphI 153 GCATGC GCATG^C
SspI 3191 AATATT AAT^ATT
XbaI 2640 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Asp700I 3898 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4925 GAACAGCTTC
BglI 447 GCCCGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2404 GCCTGGGAGGC
BsiEI 282 CGGCCG CG[GA][CT]^CG
286 CGGCCG
BsrBI 1768 CCGCTC CCGCTC(-3/-3)
2611 GAGCGG
BssHII 487 GCGCGC G^CGCGC
502 GCGCGC
DsaI 1119 CCATGG C^C[GA][CT]GG
1158 CCGTGG
EagI 282 CGGCCG C^GGCCG
286 CGGCCG
EclXI 282 CGGCCG C^GGCCG
286 CGGCCG
Eco52I 282 CGGCCG C^GGCCG
286 CGGCCG
PflMI 588 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2220 CCAGAGGGTGG
SbfI 1728 CCTGCAGG CCTGCA^GG
5053 CCTGCAGG
Sse8387I 1728 CCTGCAGG CCTGCA^GG
5053 CCTGCAGG
Van91I 588 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2220 CCAGAGGGTGG
XcmI 2103 CCAAAGCCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4284 CCACTGAGGTCATGG
XmnI 3898 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4925 GAACAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
Aor51HI ApoI Asp718I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI Eco47III EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII