################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII Acc65I AclI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvrII BamHI BanII BcgI BglI BlnI BlpI Bpu1102I BsiEI BsiWI BsmBI BssHII BstBI Bsu36I CelII CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoRI EcoRV FseI KpnI KspI MfeI MluI MunI NotI NruI NspV PacI PaeR7I PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfuI SgrAI SnaBI Sse8387I StuI XhoI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 3679 GTATAC GT^[AC][GT]AC AflII 824 CTTAAG C^TTAAG AgeI 2436 ACCGGT A^CCGGT Aor51HI 2049 AGCGCT AGC^GCT BbeI 22 GGCGCC GGCGC^C BbrPI 4525 CACGTG CAC^GTG BbvCI 1633 CCTCAGC CCTCAGC(-5/-2) BfrI 824 CTTAAG C^TTAAG BsaAI 4525 CACGTG [CT]AC^GT[GA] BsaBI 3008 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BssSI 6010 CTCGTG CACGAG(-5/-1) Bst1107I 3679 GTATAC GTA^TAC BstEII 5419 GGTTACC G^GT[ACGT]ACC BstPI 5419 GGTTACC G^GT[ACGT]ACC BstXI 1212 CCAAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3679 GTATAC GTA^TAC Eco47III 2049 AGCGCT AGC^GCT EcoO65I 5419 GGTTACC G^GT[ACGT]ACC HpaI 5686 GTTAAC GTT^AAC KasI 22 GGCGCC G^GCGCC MamI 3008 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 25 GCCGGC GCC^GGC NarI 22 GGCGCC GG^CGCC NgoMIV 25 GCCGGC G^CCGGC NheI 5226 GCTAGC G^CTAGC PflMI 3256 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2436 ACCGGT A^CCGGT PmaCI 4525 CACGTG CAC^GTG PmlI 4525 CACGTG CAC^GTG ScaI 6076 AGTACT AGT^ACT SfoI 22 GGCGCC GGC^GCC SpeI 2944 ACTAGT A^CTAGT SphI 4059 GCATGC GCATG^C SwaI 4426 ATTTAAAT ATTT^AAAT Van91I 3256 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2254 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AspHI 1010 GAGCAC G[AT]GC[AT]^C 1673 GTGCTC AspI 382 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4457 GACTGAGTC AviII 2613 TGCGCA TGC^GCA 5505 TGCGCA BalI 2550 TGGCCA TGG^CCA 2766 TGGCCA BanI 22 GGCGCC G^G[CT][GA]CC 3975 GGCACC BglII 115 AGATCT A^GATCT 700 AGATCT BsgI 1998 CTGCAC GTGCAG(16/14) 4004 CTGCAC BsiHKAI 1010 GAGCAC G[AT]GC[AT]^C 1673 GTGCTC BspDI 3051 ATCGAT AT^CGAT 5931 ATCGAT BspHI 454 TCATGA T^CATGA 4453 TCATGA BspLU11I 4292 ACATGT A^CATGT 4472 ACATGT BsrBI 1005 GAGCGG CCGCTC(-3/-3) 3266 GAGCGG BsrDI 2863 CATTGC GCAATG(2/0) 5785 GCAATG ClaI 3051 ATCGAT AT^CGAT 5931 ATCGAT EcoNI 2452 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5324 CCTTTATGAGG FspI 2613 TGCGCA TGC^GCA 5505 TGCGCA HaeII 22 GGCGCC [GA]GCGC^[CT] 2049 AGCGCT MluNI 2550 TGGCCA TGG^CCA 2766 TGGCCA MscI 2550 TGGCCA TGG^CCA 2766 TGGCCA NcoI 546 CCATGG C^CATGG 3530 CCATGG NdeI 2525 CATATG CA^TATG 3118 CATATG PflFI 382 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4457 GACTGAGTC PstI 725 CTGCAG CTGCA^G 3205 CTGCAG RcaI 454 TCATGA T^CATGA 4453 TCATGA SexAI 654 ACCAGGT A^CC[AT]GGT 5117 ACCAGGT SmaI 49 CCCGGG CCC^GGG 96 CCCGGG Tth111I 382 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4457 GACTGAGTC XmaCI 49 CCCGGG C^CCGGG 96 CCCGGG XmaI 49 CCCGGG C^CCGGG 96 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccII AccIII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AvaI AvaII BbsI BbvI BciVI BclI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspEI BspMI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI