##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       Acc65I      AclI        AhdI        Alw44I      
AocI        ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AvrII       BamHI       
BanII       BcgI        BglI        BlnI        BlpI        
Bpu1102I    BsiEI       BsiWI       BsmBI       BssHII      
BstBI       Bsu36I      CelII       CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoRI       EcoRV       FseI        KpnI        
KspI        MfeI        MluI        MunI        NotI        
NruI        NspV        PacI        PaeR7I      PmeI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SfiI        SfuI        SgrAI       SnaBI       Sse8387I    
StuI        XhoI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI         3679  GTATAC               GT^[AC][GT]AC
AflII         824  CTTAAG               C^TTAAG
AgeI         2436  ACCGGT               A^CCGGT
Aor51HI      2049  AGCGCT               AGC^GCT
BbeI           22  GGCGCC               GGCGC^C
BbrPI        4525  CACGTG               CAC^GTG
BbvCI        1633  CCTCAGC              CCTCAGC(-5/-2)
BfrI          824  CTTAAG               C^TTAAG
BsaAI        4525  CACGTG               [CT]AC^GT[GA]
BsaBI        3008  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BssSI        6010  CTCGTG               CACGAG(-5/-1)
Bst1107I     3679  GTATAC               GTA^TAC
BstEII       5419  GGTTACC              G^GT[ACGT]ACC
BstPI        5419  GGTTACC              G^GT[ACGT]ACC
BstXI        1212  CCAAAAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      3679  GTATAC               GTA^TAC
Eco47III     2049  AGCGCT               AGC^GCT
EcoO65I      5419  GGTTACC              G^GT[ACGT]ACC
HpaI         5686  GTTAAC               GTT^AAC
KasI           22  GGCGCC               G^GCGCC
MamI         3008  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI           25  GCCGGC               GCC^GGC
NarI           22  GGCGCC               GG^CGCC
NgoMIV         25  GCCGGC               G^CCGGC
NheI         5226  GCTAGC               G^CTAGC
PflMI        3256  CCACCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        2436  ACCGGT               A^CCGGT
PmaCI        4525  CACGTG               CAC^GTG
PmlI         4525  CACGTG               CAC^GTG
ScaI         6076  AGTACT               AGT^ACT
SfoI           22  GGCGCC               GGC^GCC
SpeI         2944  ACTAGT               A^CTAGT
SphI         4059  GCATGC               GCATG^C
SwaI         4426  ATTTAAAT             ATTT^AAAT
Van91I       3256  CCACCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2254  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AspHI        1010  GAGCAC               G[AT]GC[AT]^C
             1673  GTGCTC              
AspI          382  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4457  GACTGAGTC           
AviII        2613  TGCGCA               TGC^GCA
             5505  TGCGCA              
BalI         2550  TGGCCA               TGG^CCA
             2766  TGGCCA              
BanI           22  GGCGCC               G^G[CT][GA]CC
             3975  GGCACC              
BglII         115  AGATCT               A^GATCT
              700  AGATCT              
BsgI         1998  CTGCAC               GTGCAG(16/14)
             4004  CTGCAC              
BsiHKAI      1010  GAGCAC               G[AT]GC[AT]^C
             1673  GTGCTC              
BspDI        3051  ATCGAT               AT^CGAT
             5931  ATCGAT              
BspHI         454  TCATGA               T^CATGA
             4453  TCATGA              
BspLU11I     4292  ACATGT               A^CATGT
             4472  ACATGT              
BsrBI        1005  GAGCGG               CCGCTC(-3/-3)
             3266  GAGCGG              
BsrDI        2863  CATTGC               GCAATG(2/0)
             5785  GCAATG              
ClaI         3051  ATCGAT               AT^CGAT
             5931  ATCGAT              
EcoNI        2452  CCTCAAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5324  CCTTTATGAGG         
FspI         2613  TGCGCA               TGC^GCA
             5505  TGCGCA              
HaeII          22  GGCGCC               [GA]GCGC^[CT]
             2049  AGCGCT              
MluNI        2550  TGGCCA               TGG^CCA
             2766  TGGCCA              
MscI         2550  TGGCCA               TGG^CCA
             2766  TGGCCA              
NcoI          546  CCATGG               C^CATGG
             3530  CCATGG              
NdeI         2525  CATATG               CA^TATG
             3118  CATATG              
PflFI         382  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4457  GACTGAGTC           
PstI          725  CTGCAG               CTGCA^G
             3205  CTGCAG              
RcaI          454  TCATGA               T^CATGA
             4453  TCATGA              
SexAI         654  ACCAGGT              A^CC[AT]GGT
             5117  ACCAGGT             
SmaI           49  CCCGGG               CCC^GGG
               96  CCCGGG              
Tth111I       382  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4457  GACTGAGTC           
XmaCI          49  CCCGGG               C^CCGGG
               96  CCCGGG              
XmaI           49  CCCGGG               C^CCGGG
               96  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

AccII       AccIII      AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
ApoI        Asp700I     AvaI        AvaII       BbsI        
BbvI        BciVI       BclI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspEI       BspMI       BsrFI       
BsrGI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MroI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NsiI        NspI        PleI        PpuMI       PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspBI       SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
XmnI