##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AspI AviII AvrII BbeI
BbrPI BglI BlnI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstXI BstZ17I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRV FauI FseI FspI HaeII
KasI KspI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 397 GGTACC G^GTACC
AccI 1242 GTCTAC GT^[AC][GT]AC
AccII 4 CGCG CG^CG
AflII 1159 CTTAAG C^TTAAG
AflIII 2327 ACATGT A^C[GA][CT]GT
Asp700I 281 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 397 GGTACC G^GTACC
AvaI 324 CTCGAG C^[CT]CG[GA]G
AvaII 4373 GGACC G^G[AT]CC
BamHI 1515 GGATCC G^GATCC
BbvCI 3185 GCTGAGG CCTCAGC(-5/-2)
BcgI 2419 CGAAGGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1532 GGATAC GTATCC(6/5)
BcnI 3572 CCCGG CC^[GC]GG
BfrI 1159 CTTAAG C^TTAAG
BlpI 2545 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2545 GCTCAGC GC^T[ACGT]AGC
BsaAI 3256 CACGTA [CT]AC^GT[GA]
BsiEI 12 CGACCG CG[GA][CT]^CG
BsmFI 3440 GTCCC GGGAC(10/14)
BsoBI 324 CTCGAG C^[CT]CG[GA]G
BspLU11I 2327 ACATGT A^CATGT
BssSI 2321 CACGAG CACGAG(-5/-1)
BstAPI 3645 GCACATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 4 CGCG CG^CG
CelII 2545 GCTCAGC GC^T[ACGT]AGC
CfoI 3 GCGC GCG^C
DraII 4114 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 4114 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 2127 ATGCAT ATGCA^T
HhaI 3 GCGC GCG^C
HinP1I 3 GCGC G^CGC
HpaI 119 GTTAAC GTT^AAC
KpnI 397 GGTACC GGTAC^C
MvnI 4 CGCG CG^CG
NciI 3572 CCCGG CC^[GC]GG
NcoI 18 CCATGG C^CATGG
NsiI 2127 ATGCAT ATGCA^T
PaeR7I 324 CTCGAG C^TCGAG
SacI 3149 GAGCTC GAGCT^C
SpeI 2729 ACTAGT A^CTAGT
XcmI 2922 CCAATAAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 324 CTCGAG C^TCGAG
XmnI 281 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 1981 GACGCC G[GA]^CG[CT]C
2179 GACGCC
BalI 1066 TGGCCA TGG^CCA
2958 TGGCCA
BanII 2226 GAGCCC G[GA]GC[CT]^C
3149 GAGCTC
BclI 672 TGATCA T^GATCA
1080 TGATCA
BpmI 207 CTGGAG CTGGAG(16/14)
1260 CTGGAG
BsaBI 1502 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2255 GATGCAAATC
BsaHI 1981 GACGCC G[GA]^CG[CT]C
2179 GACGCC
BsaI 3170 GAGACC GGTCTC(1/5)
3394 GAGACC
BspHI 2722 TCATGA T^CATGA
3069 TCATGA
BstEII 1565 GGTCACC G^GT[ACGT]ACC
1700 GGTCACC
BstPI 1565 GGTCACC G^GT[ACGT]ACC
1700 GGTCACC
DraI 3335 TTTAAA TTT^AAA
4379 TTTAAA
DsaI 18 CCATGG C^C[GA][CT]GG
964 CCACGG
EaeI 1066 TGGCCA [CT]^GGCC[GA]
2958 TGGCCA
EcoO65I 1565 GGTCACC G^GT[ACGT]ACC
1700 GGTCACC
EcoRI 3342 GAATTC G^AATTC
3354 GAATTC
FbaI 672 TGATCA T^GATCA
1080 TGATCA
HapII 2176 CCGG C^CGG
3573 CCGG
HgaI 1981 GACGC GACGC(5/10)
2179 GACGC
Hin1I 1981 GACGCC G[GA]^CG[CT]C
2179 GACGCC
HincII 119 GTTAAC GT[CT]^[GA]AC
241 GTTGAC
HindII 119 GTTAAC GT[CT]^[GA]AC
241 GTTGAC
HpaII 2176 CCGG C^CGG
3573 CCGG
MamI 1502 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2255 GATGCAAATC
MluNI 1066 TGGCCA TGG^CCA
2958 TGGCCA
MscI 1066 TGGCCA TGG^CCA
2958 TGGCCA
MspI 2176 CCGG C^CGG
3573 CCGG
RcaI 2722 TCATGA T^CATGA
3069 TCATGA
ScaI 3126 AGTACT AGT^ACT
3986 AGTACT
SspI 100 AATATT AAT^ATT
512 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AseI AsnI
AspHI BanI BbsI BbvI BfaI
BglII BmyI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII EarI Eco81I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII