################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII Acc65I AccIII AgeI AscI AseI AsnI Asp700I Asp718I AspI AviII BamHI BbeI BbrPI BclI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraIII DrdI EcoRI FbaI FspI HpaI KasI KpnI MamI MfeI MluI MroI MunI NarI NdeI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI PvuI RcaI RsrII SalI SbfI SexAI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccI 2295 GTAGAC GT^[AC][GT]AC AclI 3829 AACGTT AA^CGTT AcyI 3741 GACGCC G[GA]^CG[CT]C Alw44I 1566 GTGCAC G^TGCAC Aor51HI 5642 AGCGCT AGC^GCT ApaLI 1566 GTGCAC G^TGCAC BanI 4618 GGTGCC G^G[CT][GA]CC BciVI 4243 GTATCC GTATCC(6/5) BsaHI 3741 GACGCC G[GA]^CG[CT]C BstEII 2040 GGTCACC G^GT[ACGT]ACC BstPI 2040 GGTCACC G^GT[ACGT]ACC DraI 1928 TTTAAA TTT^AAA Eco47III 5642 AGCGCT AGC^GCT EcoO65I 2040 GGTCACC G^GT[ACGT]ACC EcoRV 4993 GATATC GAT^ATC EcoT22I 1462 ATGCAT ATGCA^T FseI 2660 GGCCGGCC GGCCGG^CC HaeII 5642 AGCGCT [GA]GCGC^[CT] Hin1I 3741 GACGCC G[GA]^CG[CT]C HincII 464 GTCAAC GT[CT]^[GA]AC HindII 464 GTCAAC GT[CT]^[GA]AC HindIII 5205 AAGCTT A^AGCTT KspI 23 CCGCGG CCGC^GG NsiI 1462 ATGCAT ATGCA^T PshAI 1626 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 3829 AACGTT AA^CGTT SacI 1775 GAGCTC GAGCT^C SacII 23 CCGCGG CCGC^GG ScaI 2611 AGTACT AGT^ACT SpeI 1843 ACTAGT A^CTAGT SspI 3907 AATATT AAT^ATT SwaI 1927 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AflII 2385 CTTAAG C^TTAAG 4442 CTTAAG AocI 350 CCTGAGG CC^T[ACGT]AGG 5665 CCTGAGG AvrII 2127 CCTAGG C^CTAGG 3633 CCTAGG BbsI 4785 GAAGAC GAAGAC(2/6) 2782 GTCTTC BcgI 5699 CGATTGCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 269 GCAGGAACCTCG BfrI 2385 CTTAAG C^TTAAG 4442 CTTAAG BlnI 2127 CCTAGG C^CTAGG 3633 CCTAGG BlpI 4139 GCTGAGC GC^T[ACGT]AGC 5082 GCTCAGC Bpu1102I 4139 GCTGAGC GC^T[ACGT]AGC 5082 GCTCAGC BpuAI 4785 GAAGAC GAAGAC(2/6) 2782 GTCTTC BsaI 695 GAGACC GGTCTC(1/5) 1666 GGTCTC BsiEI 304 CGGCCG CG[GA][CT]^CG 2663 CGGCCG BsmI 1460 GAATGC GAATGC(1/-1) 4061 GCATTC BspLU11I 1562 ACATGT A^CATGT 2495 ACATGT BsrFI 302 GCCGGC [GA]^CCGG[CT] 2661 GCCGGC Bsu36I 350 CCTGAGG CC^T[ACGT]AGG 5665 CCTGAGG CelII 4139 GCTGAGC GC^T[ACGT]AGC 5082 GCTCAGC Cfr10I 302 GCCGGC [GA]^CCGG[CT] 2661 GCCGGC EagI 304 CGGCCG C^GGCCG 2663 CGGCCG EclXI 304 CGGCCG C^GGCCG 2663 CGGCCG Eco52I 304 CGGCCG C^GGCCG 2663 CGGCCG Eco81I 350 CCTGAGG CC^T[ACGT]AGG 5665 CCTGAGG NaeI 302 GCCGGC GCC^GGC 2661 GCCGGC NcoI 81 CCATGG C^CATGG 5377 CCATGG NgoMIV 302 GCCGGC G^CCGGC 2661 GCCGGC NheI 4377 GCTAGC G^CTAGC 5190 GCTAGC SapI 815 GAAGAGC GCTCTTC(1/4) 3038 GCTCTTC SfiI 2132 GGCCAAGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 4679 GGCCAGCCAGGCC SphI 1762 GCATGC GCATG^C 5612 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AhdI AluI AlwI AlwNI ApaI ApoI AspEI AspHI AvaI AvaII BalI BanII BbvCI BbvI BcnI BfaI BglI BglII BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII