##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       Acc65I      AccIII      AgeI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AviII       BamHI       BbeI        BbrPI       BclI        
BsaAI       BsaBI       BsaWI       BseAI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DraIII      DrdI        
EcoRI       FbaI        FspI        HpaI        KasI        
KpnI        MamI        MfeI        MluI        MroI        
MunI        NarI        NdeI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       PvuI        
RcaI        RsrII       SalI        SbfI        SexAI       
SfoI        SfuI        SgrAI       SmaI        SnaBI       
Sse8387I    SspBI       Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AccI         2295  GTAGAC               GT^[AC][GT]AC
AclI         3829  AACGTT               AA^CGTT
AcyI         3741  GACGCC               G[GA]^CG[CT]C
Alw44I       1566  GTGCAC               G^TGCAC
Aor51HI      5642  AGCGCT               AGC^GCT
ApaLI        1566  GTGCAC               G^TGCAC
BanI         4618  GGTGCC               G^G[CT][GA]CC
BciVI        4243  GTATCC               GTATCC(6/5)
BsaHI        3741  GACGCC               G[GA]^CG[CT]C
BstEII       2040  GGTCACC              G^GT[ACGT]ACC
BstPI        2040  GGTCACC              G^GT[ACGT]ACC
DraI         1928  TTTAAA               TTT^AAA
Eco47III     5642  AGCGCT               AGC^GCT
EcoO65I      2040  GGTCACC              G^GT[ACGT]ACC
EcoRV        4993  GATATC               GAT^ATC
EcoT22I      1462  ATGCAT               ATGCA^T
FseI         2660  GGCCGGCC             GGCCGG^CC
HaeII        5642  AGCGCT               [GA]GCGC^[CT]
Hin1I        3741  GACGCC               G[GA]^CG[CT]C
HincII        464  GTCAAC               GT[CT]^[GA]AC
HindII        464  GTCAAC               GT[CT]^[GA]AC
HindIII      5205  AAGCTT               A^AGCTT
KspI           23  CCGCGG               CCGC^GG
NsiI         1462  ATGCAT               ATGCA^T
PshAI        1626  GACCAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     3829  AACGTT               AA^CGTT
SacI         1775  GAGCTC               GAGCT^C
SacII          23  CCGCGG               CCGC^GG
ScaI         2611  AGTACT               AGT^ACT
SpeI         1843  ACTAGT               A^CTAGT
SspI         3907  AATATT               AAT^ATT
SwaI         1927  ATTTAAAT             ATTT^AAAT


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AflII        2385  CTTAAG               C^TTAAG
             4442  CTTAAG              
AocI          350  CCTGAGG              CC^T[ACGT]AGG
             5665  CCTGAGG             
AvrII        2127  CCTAGG               C^CTAGG
             3633  CCTAGG              
BbsI         4785  GAAGAC               GAAGAC(2/6)
             2782  GTCTTC              
BcgI         5699  CGATTGCGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              269  GCAGGAACCTCG        
BfrI         2385  CTTAAG               C^TTAAG
             4442  CTTAAG              
BlnI         2127  CCTAGG               C^CTAGG
             3633  CCTAGG              
BlpI         4139  GCTGAGC              GC^T[ACGT]AGC
             5082  GCTCAGC             
Bpu1102I     4139  GCTGAGC              GC^T[ACGT]AGC
             5082  GCTCAGC             
BpuAI        4785  GAAGAC               GAAGAC(2/6)
             2782  GTCTTC              
BsaI          695  GAGACC               GGTCTC(1/5)
             1666  GGTCTC              
BsiEI         304  CGGCCG               CG[GA][CT]^CG
             2663  CGGCCG              
BsmI         1460  GAATGC               GAATGC(1/-1)
             4061  GCATTC              
BspLU11I     1562  ACATGT               A^CATGT
             2495  ACATGT              
BsrFI         302  GCCGGC               [GA]^CCGG[CT]
             2661  GCCGGC              
Bsu36I        350  CCTGAGG              CC^T[ACGT]AGG
             5665  CCTGAGG             
CelII        4139  GCTGAGC              GC^T[ACGT]AGC
             5082  GCTCAGC             
Cfr10I        302  GCCGGC               [GA]^CCGG[CT]
             2661  GCCGGC              
EagI          304  CGGCCG               C^GGCCG
             2663  CGGCCG              
EclXI         304  CGGCCG               C^GGCCG
             2663  CGGCCG              
Eco52I        304  CGGCCG               C^GGCCG
             2663  CGGCCG              
Eco81I        350  CCTGAGG              CC^T[ACGT]AGG
             5665  CCTGAGG             
NaeI          302  GCCGGC               GCC^GGC
             2661  GCCGGC              
NcoI           81  CCATGG               C^CATGG
             5377  CCATGG              
NgoMIV        302  GCCGGC               G^CCGGC
             2661  GCCGGC              
NheI         4377  GCTAGC               G^CTAGC
             5190  GCTAGC              
SapI          815  GAAGAGC              GCTCTTC(1/4)
             3038  GCTCTTC             
SfiI         2132  GGCCAAGGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             4679  GGCCAGCCAGGCC       
SphI         1762  GCATGC               GCATG^C
             5612  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AhdI        AluI        AlwI        AlwNI       ApaI        
ApoI        AspEI       AspHI       AvaI        AvaII       
BalI        BanII       BbvCI       BbvI        BcnI        
BfaI        BglI        BglII       BmyI        BpmI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrDI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        Eam1105I    
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII