##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
AlwNI       Aor51HI     AscI        Asp718I     BbrPI       
BcgI        BciVI       BglII       BlpI        Bpu1102I    
BsaAI       BseAI       BsiWI       BsmI        BspEI       
BspLU11I    BssHII      Bst1107I    BstZ17I     CelII       
CpoI        DraIII      DrdI        Eco47III    EcoRI       
EcoRV       EcoT22I     FseI        HpaI        KpnI        
KspI        MfeI        MroI        MunI        NcoI        
NheI        NotI        NruI        NsiI        PacI        
PinAI       PmaCI       PmeI        PmlI        Psp1406I    
RsrII       SacII       SbfI        SfiI        SgrAI       
SnaBI       SpeI        Sse8387I    SspI        SwaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AflII        2479  CTTAAG               C^TTAAG
AflIII        591  ACGCGT               A^C[GA][CT]GT
ApaI         1237  GGGCCC               GGGCC^C
Asp700I      3121  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        1523  TGCGCA               TGC^GCA
AvrII        1072  CCTAGG               C^CTAGG
BbvCI        2513  CCTCAGC              CCTCAGC(-5/-2)
BfrI         2479  CTTAAG               C^TTAAG
BglI         1299  GCCTTATGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1072  CCTAGG               C^CTAGG
BsgI         5095  CTGCAC               GTGCAG(16/14)
BsmBI         573  CGTCTC               CGTCTC(1/5)
BspDI        3218  ATCGAT               AT^CGAT
BspHI        1418  TCATGA               T^CATGA
BstAPI       1942  GCACTGCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1844  TTCGAA               TT^CGAA
ClaI         3218  ATCGAT               AT^CGAT
EagI          663  CGGCCG               C^GGCCG
EclXI         663  CGGCCG               C^GGCCG
Eco52I        663  CGGCCG               C^GGCCG
FspI         1523  TGCGCA               TGC^GCA
HincII        486  GTCGAC               GT[CT]^[GA]AC
HindII        486  GTCGAC               GT[CT]^[GA]AC
MluI          591  ACGCGT               A^CGCGT
NaeI          650  GCCGGC               GCC^GGC
NgoMIV        650  GCCGGC               G^CCGGC
NspV         1844  TTCGAA               TT^CGAA
PaeR7I       2423  CTCGAG               C^TCGAG
PflMI         990  CCAGACTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI         3220  CGATCG               CGAT^CG
RcaI         1418  TCATGA               T^CATGA
SalI          486  GTCGAC               G^TCGAC
SapI         3778  GCTCTTC              GCTCTTC(1/4)
SexAI        1032  ACCTGGT              A^CC[AT]GGT
SfuI         1844  TTCGAA               TT^CGAA
SphI         1947  GCATGC               GCATG^C
StuI         4241  AGGCCT               AGG^CCT
Van91I        990  CCAGACTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          316  TCTAGA               T^CTAGA
XhoI         2423  CTCGAG               C^TCGAG
XmnI         3121  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   46 enzymes) #
###################################

AccI          486  GTCGAC               GT^[AC][GT]AC
             1220  GTAGAC              
AcyI          566  GGCGCC               G[GA]^CG[CT]C
             1412  GGCGCC              
AhdI          993  GACTTTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2553  GACGTATGGTC         
Alw44I       1735  GTGCAC               G^TGCAC
             4958  GTGCAC              
ApaLI        1735  GTGCAC               G^TGCAC
             4958  GTGCAC              
AseI         2700  ATTAAT               AT^TAAT
             4768  ATTAAT              
AsnI         2700  ATTAAT               AT^TAAT
             4768  ATTAAT              
AspEI         993  GACTTTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2553  GACGTATGGTC         
AspI         1031  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2782  GACACTGTC           
BalI          629  TGGCCA               TGG^CCA
             4057  TGGCCA              
BamHI         810  GGATCC               G^GATCC
             2376  GGATCC              
BbeI          566  GGCGCC               GGCGC^C
             1412  GGCGCC              
BclI         1967  TGATCA               T^GATCA
             2885  TGATCA              
BsaHI         566  GGCGCC               G[GA]^CG[CT]C
             1412  GGCGCC              
BsaWI        1882  ACCGGA               [AT]^CCGG[AT]
             3086  ACCGGA              
Bsp1407I     4174  TGTACA               T^GTACA
             4293  TGTACA              
BspMI        1631  ACCTGC               ACCTGC(4/8)
             4776  GCAGGT              
BsrFI         650  GCCGGC               [GA]^CCGG[CT]
              665  GCCGGT              
BsrGI        4174  TGTACA               T^GTACA
             4293  TGTACA              
BstEII        168  GGTAACC              G^GT[ACGT]ACC
             1164  GGTGACC             
BstPI         168  GGTAACC              G^GT[ACGT]ACC
             1164  GGTGACC             
BstXI         494  CCAGCATCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4887  CCATGCAACTGG        
Cfr10I        650  GCCGGC               [GA]^CCGG[CT]
              665  GCCGGT              
DraI         5376  TTTAAA               TTT^AAA
             5412  TTTAAA              
Eam1105I      993  GACTTTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2553  GACGTATGGTC         
EcoNI        3843  CCTGGCAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4151  CCTCTAGGAGG         
EcoO65I       168  GGTAACC              G^GT[ACGT]ACC
             1164  GGTGACC             
FbaI         1967  TGATCA               T^GATCA
             2885  TGATCA              
HgaI          593  GCGTC                GACGC(5/10)
             2583  GCGTC               
Hin1I         566  GGCGCC               G[GA]^CG[CT]C
             1412  GGCGCC              
KasI          566  GGCGCC               G^GCGCC
             1412  GGCGCC              
MluNI         629  TGGCCA               TGG^CCA
             4057  TGGCCA              
MscI          629  TGGCCA               TGG^CCA
             4057  TGGCCA              
NarI          566  GGCGCC               GG^CGCC
             1412  GGCGCC              
NdeI         3056  CATATG               CA^TATG
             4579  CATATG              
PflFI        1031  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2782  GACACTGTC           
PshAI         879  GACCACGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1402  GACCCTCGTC          
PshBI        2700  ATTAAT               AT^TAAT
             4768  ATTAAT              
PstI         3554  CTGCAG               CTGCA^G
             4414  CTGCAG              
ScaI         1698  AGTACT               AGT^ACT
             3067  AGTACT              
SfoI          566  GGCGCC               GGC^GCC
             1412  GGCGCC              
SmaI          545  CCCGGG               CCC^GGG
             1160  CCCGGG              
SspBI        4174  TGTACA               T^GTACA
             4293  TGTACA              
Tth111I      1031  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2782  GACACTGTC           
XmaCI         545  CCCGGG               C^CCGGG
             1160  CCCGGG              
XmaI          545  CCCGGG               C^CCGGG
             1160  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AocI        ApoI        AspHI       AvaI        
AvaII       BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaBI       BsaI        BsaJI       BseRI       BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrBI       BsrDI       BsrI        
BssKI       BssSI       BstF5I      BstNI       BstUI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII