##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AgeI        Aor51HI     ApaI        AscI        Asp718I     
AviII       AvrII       BamHI       BbeI        BbrPI       
BcgI        BglI        BlnI        BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       BstBI       
BstUI       Cfr10I      ClaI        CpoI        DraIII      
DrdI        DsaI        EagI        EclXI       Eco47III    
Eco52I      EcoRV       FseI        FspI        HaeII       
Hin1I       KasI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshAI       
PstI        PvuI        RcaI        RsrII       SacII       
SapI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AclI         3552  AACGTT               AA^CGTT
AflII        3785  CTTAAG               C^TTAAG
AhdI          428  GACCAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        660  GTGCAC               G^TGCAC
ApaLI         660  GTGCAC               G^TGCAC
AspEI         428  GACCAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         4662  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI         1216  GGTGCC               G^G[CT][GA]CC
BbsI         1331  GTCTTC               GAAGAC(2/6)
BciVI        1930  GTATCC               GTATCC(6/5)
BfrI         3785  CTTAAG               C^TTAAG
BlpI         2757  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2757  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1331  GTCTTC               GAAGAC(2/6)
BsiEI        4571  CGGTCG               CG[GA][CT]^CG
BsrBI        4568  GAGCGG               CCGCTC(-3/-3)
BstAPI       2762  GCAAAGTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        723  GGTTACC              G^GT[ACGT]ACC
BstPI         723  GGTTACC              G^GT[ACGT]ACC
CelII        2757  GCTGAGC              GC^T[ACGT]AGC
CfoI         2411  GCGC                 GCG^C
DraII          87  AGGACCC              [GA]G^G[ACGT]CC[CT]
Eam1105I      428  GACCAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I       87  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       723  GGTTACC              G^GT[ACGT]ACC
EcoRI         795  GAATTC               G^AATTC
HgaI          138  GCGTC                GACGC(5/10)
HhaI         2411  GCGC                 GCG^C
HinP1I       2411  GCGC                 G^CGC
HpaI         1420  GTTAAC               GTT^AAC
MslI          786  CACGGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I       1046  CTCGAG               C^TCGAG
PflFI        4662  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI          87  AGGACCC              [GA]G^G[AT]CC[CT]
Psp1406I     3552  AACGTT               AA^CGTT
PvuII        1085  CAGCTG               CAG^CTG
SalI          140  GTCGAC               G^TCGAC
SexAI         429  ACCAGGT              A^CC[AT]GGT
SphI         4515  GCATGC               GCATG^C
StuI         2029  AGGCCT               AGG^CCT
SwaI         1283  ATTTAAAT             ATTT^AAAT
Tth111I      4662  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1101  CCAGGAGATGTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1046  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AflIII       1472  ACATGT               A^C[GA][CT]GT
             2439  ACATGT              
AlwNI         199  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1268  CAGATTCTG           
AseI         2082  ATTAAT               AT^TAAT
             3651  ATTAAT              
AsnI         2082  ATTAAT               AT^TAAT
             3651  ATTAAT              
BalI          887  TGGCCA               TGG^CCA
             1146  TGGCCA              
BbvCI         294  CCTCAGC              CCTCAGC(-5/-2)
             3238  GCTGAGG             
BclI         3664  TGATCA               T^GATCA
             3719  TGATCA              
BglII        2735  AGATCT               A^GATCT
             3603  AGATCT              
BpmI          955  CTCCAG               CTGGAG(16/14)
             1099  CTCCAG              
BsaI         1119  GAGACC               GGTCTC(1/5)
             3204  GGTCTC              
BseRI          49  GAGGAG               GAGGAG(10/8)
              508  GAGGAG              
BsgI          267  CTGCAC               GTGCAG(16/14)
             4552  GTGCAG              
BsmFI        2866  GGGAC                GGGAC(10/14)
             3302  GTCCC               
BsmI         3108  GAATGC               GAATGC(1/-1)
             2695  GCATTC              
BspLU11I     1472  ACATGT               A^CATGT
             2439  ACATGT              
BspMI         610  GCAGGT               ACCTGC(4/8)
             3278  GCAGGT              
Bst1107I     3636  GTATAC               GTA^TAC
             3792  GTATAC              
BstXI         878  CCATGAGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1221  CCAAGAGAATGG        
BstZ17I      3636  GTATAC               GTA^TAC
             3792  GTATAC              
EaeI          887  TGGCCA               [CT]^GGCC[GA]
             1146  TGGCCA              
EcoT22I      2416  ATGCAT               ATGCA^T
             4513  ATGCAT              
FauI         3063  CCCGC                CCCGC(4/6)
             3353  CCCGC               
FbaI         3664  TGATCA               T^GATCA
             3719  TGATCA              
HindIII      2612  AAGCTT               A^AGCTT
             4392  AAGCTT              
MaeII        2786  ACGT                 A^CGT
             3553  ACGT                
MluNI         887  TGGCCA               TGG^CCA
             1146  TGGCCA              
MscI          887  TGGCCA               TGG^CCA
             1146  TGGCCA              
MspA1I        187  CAGCGG               C[AC]G^C[GT]G
             1085  CAGCTG              
NsiI         2416  ATGCAT               ATGCA^T
             4513  ATGCAT              
PshBI        2082  ATTAAT               AT^TAAT
             3651  ATTAAT              
SacI           63  GAGCTC               GAGCT^C
             4152  GAGCTC              
ScaI         4384  AGTACT               AGT^ACT
             4430  AGTACT              
SmaI          463  CCCGGG               CCC^GGG
             2496  CCCGGG              
SspI          696  AATATT               AAT^ATT
             2219  AATATT              
XmaCI         463  CCCGGG               C^CCGGG
             2496  CCCGGG              
XmaI          463  CCCGGG               C^CCGGG
             2496  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AocI        ApoI        Asp700I     AspHI       
AvaI        AvaII       BanII       BbvI        BcnI        
BfaI        BmyI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Bsu36I      
Cac8I       Cfr13I      DdeI        DpnII       DraI        
EarI        Eco81I      EcoNI       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XbaI        XhoII       XmnI