##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Aor51HI ApaI AscI Asp700I AspEI
AviII BbeI BcgI BglI BlpI
Bpu1102I BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I BsrBI BsrDI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FseI
FspI HaeII KasI KspI MluI
MroI NarI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SnaBI SpeI Sse8387I
StuI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 4393 GGTACC G^GTACC
AccI 3664 GTCTAC GT^[AC][GT]AC
AcyI 4528 GACGCC G[GA]^CG[CT]C
AflII 4354 CTTAAG C^TTAAG
AflIII 2794 ACACGT A^C[GA][CT]GT
Alw44I 1745 GTGCAC G^TGCAC
ApaLI 1745 GTGCAC G^TGCAC
AseI 5296 ATTAAT AT^TAAT
AsnI 5296 ATTAAT AT^TAAT
Asp718I 4393 GGTACC G^GTACC
AspI 4137 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 792 CCCGGG C^[CT]CG[GA]G
AvrII 3119 CCTAGG C^CTAGG
BanII 731 GGGCTC G[GA]GC[CT]^C
BbrPI 3582 CACGTG CAC^GTG
BbsI 3404 GAAGAC GAAGAC(2/6)
BciVI 2712 GGATAC GTATCC(6/5)
BfrI 4354 CTTAAG C^TTAAG
BlnI 3119 CCTAGG C^CTAGG
BpuAI 3404 GAAGAC GAAGAC(2/6)
BsaAI 3582 CACGTG [CT]AC^GT[GA]
BsaBI 2118 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4528 GACGCC G[GA]^CG[CT]C
BsoBI 792 CCCGGG C^[CT]CG[GA]G
CfoI 1753 GCGC GCG^C
EcoRI 325 GAATTC G^AATTC
HgaI 4528 GACGC GACGC(5/10)
HhaI 1753 GCGC GCG^C
Hin1I 4528 GACGCC G[GA]^CG[CT]C
HinP1I 1753 GCGC G^CGC
HindIII 5221 AAGCTT A^AGCTT
HpaI 5129 GTTAAC GTT^AAC
KpnI 4393 GGTACC GGTAC^C
MamI 2118 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2820 CAATTG C^AATTG
MunI 2820 CAATTG C^AATTG
NaeI 288 GCCGGC GCC^GGC
NdeI 3171 CATATG CA^TATG
NgoMIV 288 GCCGGC G^CCGGC
NheI 1020 GCTAGC G^CTAGC
PflFI 4137 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3582 CACGTG CAC^GTG
PmlI 3582 CACGTG CAC^GTG
PshAI 1851 GACACGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5296 ATTAAT AT^TAAT
SexAI 3161 ACCAGGT A^CC[AT]GGT
SgrAI 603 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 792 CCCGGG CCC^GGG
SspI 5341 AATATT AAT^ATT
Tth111I 4137 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 792 CCCGGG C^CCGGG
XmaI 792 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 80 CGCG CG^CG
1752 CGCG
AocI 3594 CCTGAGG CC^T[ACGT]AGG
4972 CCTTAGG
BalI 528 TGGCCA TGG^CCA
4552 TGGCCA
BamHI 681 GGATCC G^GATCC
5183 GGATCC
BsmI 634 GAATGC GAATGC(1/-1)
1180 GCATTC
Bsp1407I 1002 TGTACA T^GTACA
4461 TGTACA
BsrGI 1002 TGTACA T^GTACA
4461 TGTACA
BssSI 1853 CACGAG CACGAG(-5/-1)
278 CTCGTG
BstUI 80 CGCG CG^CG
1752 CGCG
BstXI 549 CCAAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
728 CCAGGGCTCTGG
Bsu36I 3594 CCTGAGG CC^T[ACGT]AGG
4972 CCTTAGG
DraI 3021 TTTAAA TTT^AAA
3215 TTTAAA
Eco81I 3594 CCTGAGG CC^T[ACGT]AGG
4972 CCTTAGG
HincII 449 GTCAAC GT[CT]^[GA]AC
5129 GTTAAC
HindII 449 GTCAAC GT[CT]^[GA]AC
5129 GTTAAC
MluNI 528 TGGCCA TGG^CCA
4552 TGGCCA
MscI 528 TGGCCA TGG^CCA
4552 TGGCCA
MvnI 80 CGCG CG^CG
1752 CGCG
SspBI 1002 TGTACA T^GTACA
4461 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BbvCI BbvI BclI BcnI BfaI
BglII BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspHI
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoII