##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AocI Aor51HI ApaI AscI Asp718I
AviII AvrII BbeI BbrPI BglI
BlnI BsaAI BsaHI BseAI BsiEI
BsiWI BspDI BspEI BspHI BsrBI
BsrFI BssHII BssSI BstAPI BstBI
BstEII BstPI Bsu36I Cfr10I ClaI
CpoI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I FseI FspI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PvuI RcaI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I StuI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccII 3133 CGCG CG^CG
AclI 1005 AACGTT AA^CGTT
AflII 2986 CTTAAG C^TTAAG
AflIII 1039 ACATGT A^C[GA][CT]GT
AhdI 1760 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1760 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 5694 CTCGGG C^[CT]CG[GA]G
BalI 5846 TGGCCA TGG^CCA
BanI 3065 GGTGCC G^G[CT][GA]CC
BciVI 5330 GGATAC GTATCC(6/5)
BcnI 3118 CCGGG CC^[GC]GG
BfrI 2986 CTTAAG C^TTAAG
BsaWI 3046 ACCGGA [AT]^CCGG[AT]
BsmBI 17 GAGACG CGTCTC(1/5)
BsmI 3876 GAATGC GAATGC(1/-1)
BsoBI 5694 CTCGGG C^[CT]CG[GA]G
BspLU11I 1039 ACATGT A^CATGT
BstUI 3133 CGCG CG^CG
BstXI 5818 CCAGTAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 4452 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4311 GACATTATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4293 CCATGG C^C[GA][CT]GG
EaeI 5846 TGGCCA [CT]^GGCC[GA]
Eam1105I 1760 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2298 GATATC GAT^ATC
EcoT22I 4254 ATGCAT ATGCA^T
FauI 118 GCGGG CCCGC(4/6)
MluNI 5846 TGGCCA TGG^CCA
MscI 5846 TGGCCA TGG^CCA
MvnI 3133 CGCG CG^CG
NciI 3118 CCGGG CC^[GC]GG
NcoI 4293 CCATGG C^CATGG
NheI 3799 GCTAGC G^CTAGC
NsiI 4254 ATGCAT ATGCA^T
Psp1406I 1005 AACGTT AA^CGTT
SapI 3180 GCTCTTC GCTCTTC(1/4)
ScaI 3622 AGTACT AGT^ACT
SexAI 4048 ACCTGGT A^CC[AT]GGT
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 4828 GTGCAC G^TGCAC
5487 GTGCAC
AlwNI 918 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
5968 CAGCTGCTG
ApaLI 4828 GTGCAC G^TGCAC
5487 GTGCAC
BamHI 1016 GGATCC G^GATCC
3505 GGATCC
BbvCI 915 CCTCAGC CCTCAGC(-5/-2)
3500 GCTGAGG
BcgI 2297 CGATATCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3899 GCAAATAAATCG
BglII 962 AGATCT A^GATCT
1441 AGATCT
BlpI 859 GCTTAGC GC^T[ACGT]AGC
2884 GCTTAGC
Bpu1102I 859 GCTTAGC GC^T[ACGT]AGC
2884 GCTTAGC
BsaBI 1071 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1547 GATATGAATC
BsgI 532 CTGCAC GTGCAG(16/14)
4642 CTGCAC
BsrDI 5168 CATTGC GCAATG(2/0)
1881 GCAATG
Bst1107I 453 GTATAC GTA^TAC
1324 GTATAC
BstZ17I 453 GTATAC GTA^TAC
1324 GTATAC
CelII 859 GCTTAGC GC^T[ACGT]AGC
2884 GCTTAGC
DraI 4520 TTTAAA TTT^AAA
5128 TTTAAA
EcoNI 1402 CCTAGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3724 CCTGGTTCAGG
EcoRI 1257 GAATTC G^AATTC
2070 GAATTC
MamI 1071 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1547 GATATGAATC
NspI 148 ACATGC [GA]CATG^[CT]
1039 ACATGT
PflMI 865 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3056 CCAGTATTTGG
Van91I 865 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3056 CCAGTATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AspI AvaII BanII BbsI
BbvI BclI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeI
NdeII NlaIII NlaIV PflFI PleI
PpuMI PshBI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SpeI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XbaI XcmI XhoII XmnI