##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII AcyI AflII AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AspEI AspI AvaII AvrII BalI
BbeI BbrPI BcgI BciVI BclI
BfrI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsiEI BsiWI
BsmFI Bsp1407I BspDI BspHI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I CelII ClaI CpoI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
EcoT14I FbaI FseI HgaI Hin1I
HindIII HpaI KasI KspI MamI
MfeI MluI MluNI MscI MunI
NarI NcoI NdeI NheI NotI
NruI NspV PaeR7I PflFI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI RcaI RsrII SacI SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI StyI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 415 GGTACC G^GTACC
AccII 94 CGCG CG^CG
AccIII 116 TCCGGA T^CCGGA
AclI 42 AACGTT AA^CGTT
AflIII 2111 ACATGT A^C[GA][CT]GT
AgeI 1134 ACCGGT A^CCGGT
ApaI 288 GGGCCC GGGCC^C
Asp718I 415 GGTACC G^GTACC
AviII 409 TGCGCA TGC^GCA
BbvCI 267 GCTGAGG CCTCAGC(-5/-2)
BglII 889 AGATCT A^GATCT
BseAI 116 TCCGGA T^CCGGA
BspEI 116 TCCGGA T^CCGGA
BspLU11I 2111 ACATGT A^CATGT
BspMI 412 GCAGGT ACCTGC(4/8)
BsrDI 1505 CATTGC GCAATG(2/0)
BstEII 243 GGTGACC G^GT[ACGT]ACC
BstPI 243 GGTGACC G^GT[ACGT]ACC
BstUI 94 CGCG CG^CG
DraI 1910 TTTAAA TTT^AAA
DraII 287 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 384 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 48 CCGTGG C^C[GA][CT]GG
EcoO109I 287 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 243 GGTGACC G^GT[ACGT]ACC
EcoT22I 1371 ATGCAT ATGCA^T
FspI 409 TGCGCA TGC^GCA
KpnI 415 GGTACC GGTAC^C
MaeII 43 ACGT A^CGT
MroI 116 TCCGGA T^CCGGA
MslI 1973 CATTGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 856 CAGCTG C[AC]G^C[GT]G
MvnI 94 CGCG CG^CG
NaeI 263 GCCGGC GCC^GGC
NgoMIV 263 GCCGGC G^CCGGC
NsiI 1371 ATGCAT ATGCA^T
PacI 2066 TTAATTAA TTAAT^TAA
PinAI 1134 ACCGGT A^CCGGT
Psp1406I 42 AACGTT AA^CGTT
PvuII 856 CAGCTG CAG^CTG
SapI 1589 GCTCTTC GCTCTTC(1/4)
StuI 2421 AGGCCT AGG^CCT
XcmI 1720 CCAAGAAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 690 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
842 CAGGCACTG
Asp700I 1101 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1644 GAAGATGTTC
BamHI 1523 GGATCC G^GATCC
2046 GGATCC
BanI 237 GGTGCC G^G[CT][GA]CC
415 GGTACC
BbsI 903 GAAGAC GAAGAC(2/6)
1849 GTCTTC
BglI 30 GCCGCTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
281 GCCCGAGGGGC
BpuAI 903 GAAGAC GAAGAC(2/6)
1849 GTCTTC
BsaI 1187 GAGACC GGTCTC(1/5)
1609 GAGACC
BsaWI 116 TCCGGA [AT]^CCGG[AT]
1134 ACCGGT
BsmBI 1689 CGTCTC CGTCTC(1/5)
1358 GAGACG
BsrBI 210 GAGCGG CCGCTC(-3/-3)
304 GAGCGG
FauI 95 GCGGG CCCGC(4/6)
372 GCGGG
HincII 1440 GTCAAC GT[CT]^[GA]AC
2027 GTCAAC
HindII 1440 GTCAAC GT[CT]^[GA]AC
2027 GTCAAC
NspI 1469 ACATGC [GA]CATG^[CT]
2111 ACATGT
PflMI 248 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
689 CCAGCATCTGG
PstI 1159 CTGCAG CTGCA^G
1711 CTGCAG
ScaI 1579 AGTACT AGT^ACT
2199 AGTACT
SexAI 385 ACCTGGT A^CC[AT]GGT
2242 ACCTGGT
SmaI 151 CCCGGG CCC^GGG
233 CCCGGG
SspI 1930 AATATT AAT^ATT
2307 AATATT
Van91I 248 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
689 CCAGCATCTGG
XmaCI 151 CCCGGG C^CCGGG
233 CCCGGG
XmaI 151 CCCGGG C^CCGGG
233 CCCGGG
XmnI 1101 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1644 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AocI ApoI AspHI AvaI
BanII BbvI BcnI BfaI BmyI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII EarI Eco81I
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII