##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I AspEI AspI AviII BbrPI
BciVI BclI BfrI BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
Eco47III EcoO65I EcoRV EcoT22I FbaI
FspI HindIII HpaI MamI MfeI
MluI MroI MunI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI ScaI SfuI SgrAI
SnaBI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 22 enzymes) #
###################################
AatII 5142 GACGTC GACGT^C
Acc65I 1574 GGTACC G^GTACC
AflIII 3457 ACATGT A^C[GA][CT]GT
Asp718I 1574 GGTACC G^GTACC
BsaAI 3275 TACGTG [CT]AC^GT[GA]
BsmBI 1421 GAGACG CGTCTC(1/5)
BspLU11I 3457 ACATGT A^CATGT
DraIII 3799 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 138 GAATTC G^AATTC
FseI 9 GGCCGGCC GGCCGG^CC
HincII 1319 GTTGAC GT[CT]^[GA]AC
HindII 1319 GTTGAC GT[CT]^[GA]AC
KpnI 1574 GGTACC GGTAC^C
KspI 419 CCGCGG CCGC^GG
MslI 1730 CACACCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 5197 CATATG CA^TATG
NspI 3457 ACATGT [GA]CATG^[CT]
PaeR7I 3401 CTCGAG C^TCGAG
SacII 419 CCGCGG CCGC^GG
SpeI 1879 ACTAGT A^CTAGT
TfiI 916 GATTC G^A[AT]TC
XhoI 3401 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Alw44I 2361 GTGCAC G^TGCAC
4455 GTGCAC
ApaLI 2361 GTGCAC G^TGCAC
4455 GTGCAC
BamHI 166 GGATCC G^GATCC
4189 GGATCC
BbvCI 296 CCTCAGC CCTCAGC(-5/-2)
4745 CCTCAGC
BsaI 1682 GGTCTC GGTCTC(1/5)
4980 GGTCTC
BsrFI 10 GCCGGC [GA]^CCGG[CT]
2962 GCCGGC
BssHII 1435 GCGCGC G^CGCGC
2557 GCGCGC
BstAPI 2581 GCAGGTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3335 GCAGGGGGTGC
Cfr10I 10 GCCGGC [GA]^CCGG[CT]
2962 GCCGGC
NaeI 10 GCCGGC GCC^GGC
2962 GCCGGC
NcoI 1515 CCATGG C^CATGG
3840 CCATGG
NgoMIV 10 GCCGGC G^CCGGC
2962 GCCGGC
SapI 1564 GAAGAGC GCTCTTC(1/4)
2651 GAAGAGC
SexAI 3562 ACCAGGT A^CC[AT]GGT
4976 ACCAGGT
SfiI 1982 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3046 GGCCGGGGTGGCC
SmaI 332 CCCGGG CCC^GGG
3177 CCCGGG
XmaCI 332 CCCGGG C^CCGGG
3177 CCCGGG
XmaI 332 CCCGGG C^CCGGG
3177 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII AvrII
BalI BanI BanII BbeI BbsI
BbvI BcgI BcnI BfaI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PshAI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII