##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AflII AgeI Aor51HI AseI
AsnI Asp718I AspI AvrII BbeI
BfrI BglII BlnI BlpI Bpu1102I
BsiWI Bsp1407I BspDI BspLU11I BsrGI
BstAPI BstBI CelII ClaI CpoI
EagI EclXI Eco47III Eco52I EcoRV
FseI HpaI KasI KpnI KspI
NarI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 5036 GACGTC GACGT^C
AccIII 3821 TCCGGA T^CCGGA
AclI 5185 AACGTT AA^CGTT
AflIII 2691 ACGCGT A^C[GA][CT]GT
AhdI 3997 GACTTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 492 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2339 GGGCCC GGGCC^C
AscI 1649 GGCGCGCC GG^CGCGCC
AspEI 3997 GACTTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2696 TGCGCA TGC^GCA
BamHI 3334 GGATCC G^GATCC
BanI 183 GGCACC G^G[CT][GA]CC
BbrPI 3365 CACGTG CAC^GTG
BciVI 4435 GTATCC GTATCC(6/5)
BclI 950 TGATCA T^GATCA
BsaAI 3365 CACGTG [CT]AC^GT[GA]
BseAI 3821 TCCGGA T^CCGGA
BsiEI 5244 CGATCG CG[GA][CT]^CG
BspEI 3821 TCCGGA T^CCGGA
BspHI 1294 TCATGA T^CATGA
BssHII 1650 GCGCGC G^CGCGC
Bst1107I 371 GTATAC GTA^TAC
BstEII 4017 GGTGACC G^GT[ACGT]ACC
BstPI 4017 GGTGACC G^GT[ACGT]ACC
BstXI 2242 CCACCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 371 GTATAC GTA^TAC
Eam1105I 3997 GACTTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1821 CCTGCCATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4017 GGTGACC G^GT[ACGT]ACC
EcoT22I 4307 ATGCAT ATGCA^T
FbaI 950 TGATCA T^GATCA
FspI 2696 TGCGCA TGC^GCA
MfeI 946 CAATTG C^AATTG
MluI 2691 ACGCGT A^CGCGT
MroI 3821 TCCGGA T^CCGGA
MspA1I 969 CAGCTG C[AC]G^C[GT]G
MunI 946 CAATTG C^AATTG
NsiI 4307 ATGCAT ATGCA^T
PmaCI 3365 CACGTG CAC^GTG
PmlI 3365 CACGTG CAC^GTG
PshAI 3001 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5185 AACGTT AA^CGTT
PvuI 5244 CGATCG CGAT^CG
PvuII 969 CAGCTG CAG^CTG
RcaI 1294 TCATGA T^CATGA
SapI 3370 GCTCTTC GCTCTTC(1/4)
ScaI 631 AGTACT AGT^ACT
SphI 2172 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 371 GTATAC GT^[AC][GT]AC
1494 GTCTAC
AcyI 2719 GACGCC G[GA]^CG[CT]C
5036 GACGTC
Alw44I 3127 GTGCAC G^TGCAC
4032 GTGCAC
ApaLI 3127 GTGCAC G^TGCAC
4032 GTGCAC
Asp700I 1873 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4269 GAAACGGTTC
BalI 1777 TGGCCA TGG^CCA
3467 TGGCCA
BbsI 866 GAAGAC GAAGAC(2/6)
1917 GAAGAC
BglI 1602 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1723 GCCCAGATGGC
BpuAI 866 GAAGAC GAAGAC(2/6)
1917 GAAGAC
BsaBI 896 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2940 GATTAGCATC
BsaHI 2719 GACGCC G[GA]^CG[CT]C
5036 GACGTC
BsmI 4305 GAATGC GAATGC(1/-1)
1942 GCATTC
BsrBI 3971 CCGCTC CCGCTC(-3/-3)
1661 GAGCGG
DrdI 654 GACATCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3689 GACAAGGAAGTC
Hin1I 2719 GACGCC G[GA]^CG[CT]C
5036 GACGTC
MamI 896 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2940 GATTAGCATC
MluNI 1777 TGGCCA TGG^CCA
3467 TGGCCA
MscI 1777 TGGCCA TGG^CCA
3467 TGGCCA
NaeI 1624 GCCGGC GCC^GGC
1654 GCCGGC
NcoI 2248 CCATGG C^CATGG
3992 CCATGG
NdeI 871 CATATG CA^TATG
1984 CATATG
NgoMIV 1624 GCCGGC G^CCGGC
1654 GCCGGC
PstI 1413 CTGCAG CTGCA^G
4383 CTGCAG
SacI 189 GAGCTC GAGCT^C
3560 GAGCTC
SspI 781 AATATT AAT^ATT
5076 AATATT
StuI 1600 AGGCCT AGG^CCT
2091 AGGCCT
XcmI 2297 CCACTGAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4134 CCAACAGGTGCATGG
XmnI 1873 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4269 GAAACGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AocI ApoI AspHI AvaI
AvaII BanII BbvCI BbvI BcgI
BcnI BfaI BmyI BpmI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmaCI XmaI