##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AcyI        
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
AscI        AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BcgI        
BciVI       BcnI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsoBI       
BspDI       BspEI       BspHI       BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstZ17I     CelII       CfoI        
Cfr10I      ClaI        CpoI        DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      KasI        KspI        
MluI        MroI        MspA1I      MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SwaI        Tth111I     XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AclI         2216  AACGTT               AA^CGTT
AocI         2921  CCTTAGG              CC^T[ACGT]AGG
ApaI          582  GGGCCC               GGGCC^C
AseI         4104  ATTAAT               AT^TAAT
AsnI         4104  ATTAAT               AT^TAAT
AspHI        3486  GAGCAC               G[AT]GC[AT]^C
BalI         1759  TGGCCA               TGG^CCA
BbsI         2367  GTCTTC               GAAGAC(2/6)
BbvCI        4188  GCTGAGG              CCTCAGC(-5/-2)
BpuAI        2367  GTCTTC               GAAGAC(2/6)
BsaBI         494  GATTGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          887  GAGACC               GGTCTC(1/5)
BsgI          608  CTGCAC               GTGCAG(16/14)
BsiHKAI      3486  GAGCAC               G[AT]GC[AT]^C
Bsp1407I     1195  TGTACA               T^GTACA
BsrGI        1195  TGTACA               T^GTACA
BstAPI        898  GCAGCTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       2921  CCTTAGG              CC^T[ACGT]AGG
EaeI         1759  TGGCCA               [CT]^GGCC[GA]
Eco81I       2921  CCTTAGG              CC^T[ACGT]AGG
EcoNI        2921  CCTTAGGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2529  GAATTC               G^AATTC
EcoT14I      4503  CCTTGG               C^C[AT][AT]GG
FauI         2761  CCCGC                CCCGC(4/6)
HindIII      4212  AAGCTT               A^AGCTT
HpaI          221  GTTAAC               GTT^AAC
MamI          494  GATTGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1759  TGGCCA               TGG^CCA
MscI         1759  TGGCCA               TGG^CCA
PshAI         855  GACAATTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        4104  ATTAAT               AT^TAAT
Psp1406I     2216  AACGTT               AA^CGTT
SbfI          630  CCTGCAGG             CCTGCA^GG
SpeI         5380  ACTAGT               A^CTAGT
Sse8387I      630  CCTGCAGG             CCTGCA^GG
SspBI        1195  TGTACA               T^GTACA
StyI         4503  CCTTGG               C^C[AT][AT]GG
XcmI          952  CCAGCCATCTTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

Acc65I       1029  GGTACC               G^GTACC
             3630  GGTACC              
AflIII       1192  ACATGT               A^C[GA][CT]GT
             1633  ACATGT              
Asp718I      1029  GGTACC               G^GTACC
             3630  GGTACC              
BglI          617  GCCTACCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3351  GCCAGTTGGGC         
BsmI          274  GAATGC               GAATGC(1/-1)
             4920  GAATGC              
BspLU11I     1192  ACATGT               A^CATGT
             1633  ACATGT              
BspMI         278  GCAGGT               ACCTGC(4/8)
             1954  GCAGGT              
BstXI           1  CCAATTAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2504  CCAATGAAATGG        
DraIII        242  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              927  CACTAAGTG           
EcoRV         498  GATATC               GAT^ATC
             1626  GATATC              
EcoT22I      2586  ATGCAT               ATGCA^T
             4922  ATGCAT              
HapII        1232  CCGG                 C^CGG
             1243  CCGG                
HincII        221  GTTAAC               GT[CT]^[GA]AC
             4254  GTTGAC              
HindII        221  GTTAAC               GT[CT]^[GA]AC
             4254  GTTGAC              
HpaII        1232  CCGG                 C^CGG
             1243  CCGG                
KpnI         1029  GGTACC               GGTAC^C
             3630  GGTACC              
MfeI          857  CAATTG               C^AATTG
             3936  CAATTG              
MspI         1232  CCGG                 C^CGG
             1243  CCGG                
MunI          857  CAATTG               C^AATTG
             3936  CAATTG              
NsiI         2586  ATGCAT               ATGCA^T
             4922  ATGCAT              
PpuMI         648  AGGTCCC              [GA]G^G[AT]CC[CT]
             3692  GGGTCCT             
PstI          631  CTGCAG               CTGCA^G
             2619  CTGCAG              
SphI          907  GCATGC               GCATG^C
             3196  GCATGC              
StuI          615  AGGCCT               AGG^CCT
             2330  AGGCCT              
TaqI         1702  TCGA                 T^CGA
             2566  TCGA                
TthHB8I      1702  TCGA                 T^CGA
             2566  TCGA                
XbaI         2717  TCTAGA               T^CTAGA
             2868  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AciI        AcsI        AfaI        AflII       AluI        
AlwI        AlwNI       ApoI        Asp700I     AvaII       
BanI        BanII       BbvI        BclI        BfaI        
BfrI        BmyI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
DraII       EarI        EcoO109I    EcoRII      FbaI        
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        RsaI        
Sau3AI      Sau96I      ScaI        ScrFI       SfaNI       
SfcI        SmlI        SspI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Van91I      
XhoII       XmnI