################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII AccI AccII AccIII AcyI AgeI AhdI Alw44I Aor51HI ApaLI AscI AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BcnI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI KspI MluI MroI MspA1I MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AclI 2216 AACGTT AA^CGTT AocI 2921 CCTTAGG CC^T[ACGT]AGG ApaI 582 GGGCCC GGGCC^C AseI 4104 ATTAAT AT^TAAT AsnI 4104 ATTAAT AT^TAAT AspHI 3486 GAGCAC G[AT]GC[AT]^C BalI 1759 TGGCCA TGG^CCA BbsI 2367 GTCTTC GAAGAC(2/6) BbvCI 4188 GCTGAGG CCTCAGC(-5/-2) BpuAI 2367 GTCTTC GAAGAC(2/6) BsaBI 494 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 887 GAGACC GGTCTC(1/5) BsgI 608 CTGCAC GTGCAG(16/14) BsiHKAI 3486 GAGCAC G[AT]GC[AT]^C Bsp1407I 1195 TGTACA T^GTACA BsrGI 1195 TGTACA T^GTACA BstAPI 898 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 2921 CCTTAGG CC^T[ACGT]AGG EaeI 1759 TGGCCA [CT]^GGCC[GA] Eco81I 2921 CCTTAGG CC^T[ACGT]AGG EcoNI 2921 CCTTAGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2529 GAATTC G^AATTC EcoT14I 4503 CCTTGG C^C[AT][AT]GG FauI 2761 CCCGC CCCGC(4/6) HindIII 4212 AAGCTT A^AGCTT HpaI 221 GTTAAC GTT^AAC MamI 494 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1759 TGGCCA TGG^CCA MscI 1759 TGGCCA TGG^CCA PshAI 855 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4104 ATTAAT AT^TAAT Psp1406I 2216 AACGTT AA^CGTT SbfI 630 CCTGCAGG CCTGCA^GG SpeI 5380 ACTAGT A^CTAGT Sse8387I 630 CCTGCAGG CCTGCA^GG SspBI 1195 TGTACA T^GTACA StyI 4503 CCTTGG C^C[AT][AT]GG XcmI 952 CCAGCCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Acc65I 1029 GGTACC G^GTACC 3630 GGTACC AflIII 1192 ACATGT A^C[GA][CT]GT 1633 ACATGT Asp718I 1029 GGTACC G^GTACC 3630 GGTACC BglI 617 GCCTACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3351 GCCAGTTGGGC BsmI 274 GAATGC GAATGC(1/-1) 4920 GAATGC BspLU11I 1192 ACATGT A^CATGT 1633 ACATGT BspMI 278 GCAGGT ACCTGC(4/8) 1954 GCAGGT BstXI 1 CCAATTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2504 CCAATGAAATGG DraIII 242 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 927 CACTAAGTG EcoRV 498 GATATC GAT^ATC 1626 GATATC EcoT22I 2586 ATGCAT ATGCA^T 4922 ATGCAT HapII 1232 CCGG C^CGG 1243 CCGG HincII 221 GTTAAC GT[CT]^[GA]AC 4254 GTTGAC HindII 221 GTTAAC GT[CT]^[GA]AC 4254 GTTGAC HpaII 1232 CCGG C^CGG 1243 CCGG KpnI 1029 GGTACC GGTAC^C 3630 GGTACC MfeI 857 CAATTG C^AATTG 3936 CAATTG MspI 1232 CCGG C^CGG 1243 CCGG MunI 857 CAATTG C^AATTG 3936 CAATTG NsiI 2586 ATGCAT ATGCA^T 4922 ATGCAT PpuMI 648 AGGTCCC [GA]G^G[AT]CC[CT] 3692 GGGTCCT PstI 631 CTGCAG CTGCA^G 2619 CTGCAG SphI 907 GCATGC GCATG^C 3196 GCATGC StuI 615 AGGCCT AGG^CCT 2330 AGGCCT TaqI 1702 TCGA T^CGA 2566 TCGA TthHB8I 1702 TCGA T^CGA 2566 TCGA XbaI 2717 TCTAGA T^CTAGA 2868 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AcsI AfaI AflII AluI AlwI AlwNI ApoI Asp700I AvaII BanI BanII BbvI BclI BfaI BfrI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII FbaI Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII XmnI