##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AscI AseI AsnI
Asp718I AspI AvrII BbrPI BciVI
BfrI BlnI BsaBI BseAI BspDI
BspEI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI DraI DraIII
DrdI EcoO65I EcoRV FseI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MseI
MunI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI SacII SalI SfiI
SfuI SnaBI SpeI SspI SwaI
Tru9I Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 4625 CGCG CG^CG
AhdI 3732 GACGGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1510 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3732 GACGGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2286 TGCGCA TGC^GCA
BamHI 4949 GGATCC G^GATCC
BcgI 1633 GCAGCTGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3379 TGATCA T^GATCA
BglII 198 AGATCT A^GATCT
BsaAI 3521 TACGTG [CT]AC^GT[GA]
BsiWI 3900 CGTACG C^GTACG
Bsp1407I 4760 TGTACA T^GTACA
BspLU11I 1649 ACATGT A^CATGT
BsrGI 4760 TGTACA T^GTACA
BstUI 4625 CGCG CG^CG
CpoI 2514 CGGACCG CG^G[AT]CCG
EagI 2 CGGCCG C^GGCCG
Eam1105I 3732 GACGGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2 CGGCCG C^GGCCG
Eco52I 2 CGGCCG C^GGCCG
EcoRI 2876 GAATTC G^AATTC
EcoT22I 908 ATGCAT ATGCA^T
FbaI 3379 TGATCA T^GATCA
FspI 2286 TGCGCA TGC^GCA
MvnI 4625 CGCG CG^CG
NaeI 1079 GCCGGC GCC^GGC
NdeI 4321 CATATG CA^TATG
NgoMIV 1079 GCCGGC G^CCGGC
NheI 4309 GCTAGC G^CTAGC
NotI 1 GCGGCCGC GC^GGCCGC
NsiI 908 ATGCAT ATGCA^T
PaeR7I 1968 CTCGAG C^TCGAG
PshAI 64 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 2514 CGGACCG CG^G[AT]CCG
SacI 1451 GAGCTC GAGCT^C
SapI 297 GCTCTTC GCTCTTC(1/4)
SbfI 3682 CCTGCAGG CCTGCA^GG
ScaI 1908 AGTACT AGT^ACT
SgrAI 1706 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 3682 CCTGCAGG CCTGCA^GG
SspBI 4760 TGTACA T^GTACA
XhoI 1968 CTCGAG C^TCGAG
XmnI 1510 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 393 ACACGT A^C[GA][CT]GT
1649 ACATGT
Alw44I 4185 GTGCAC G^TGCAC
4605 GTGCAC
Aor51HI 1545 AGCGCT AGC^GCT
4409 AGCGCT
ApaLI 4185 GTGCAC G^TGCAC
4605 GTGCAC
BsaWI 2073 ACCGGA [AT]^CCGG[AT]
4081 ACCGGA
BsiEI 2 CGGCCG CG[GA][CT]^CG
4206 CGACCG
BsmI 1086 GCATTC GAATGC(1/-1)
4980 GCATTC
BspHI 459 TCATGA T^CATGA
3211 TCATGA
BsrBI 5 CCGCTC CCGCTC(-3/-3)
3564 CCGCTC
BsrDI 4056 CATTGC GCAATG(2/0)
791 GCAATG
Eco47III 1545 AGCGCT AGC^GCT
4409 AGCGCT
RcaI 459 TCATGA T^CATGA
3211 TCATGA
SexAI 1017 ACCTGGT A^CC[AT]GGT
4180 ACCTGGT
SmaI 229 CCCGGG CCC^GGG
1572 CCCGGG
SphI 48 GCATGC GCATG^C
3971 GCATGC
XmaCI 229 CCCGGG C^CCGGG
1572 CCCGGG
XmaI 229 CCCGGG C^CCGGG
1572 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII