##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccI AccIII AgeI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AviII AvrII BbeI
BbrPI BcgI BclI BlnI BlpI
Bpu1102I BsaBI BseAI BsiWI BspEI
BssHII Bst1107I BstBI BstZ17I Bsu36I
CelII CpoI DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoT22I FbaI
FseI FspI HaeII KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflMI PinAI PmaCI PmlI
PshBI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Van91I XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 166 GACGTC GACGT^C
AflII 4889 CTTAAG C^TTAAG
AhdI 5486 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 211 GGGCCC GGGCC^C
AspEI 5486 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4834 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 864 TGGCCA TGG^CCA
BamHI 515 GGATCC G^GATCC
BfrI 4889 CTTAAG C^TTAAG
BglII 2802 AGATCT A^GATCT
BsaAI 5698 CACGTA [CT]AC^GT[GA]
BsaI 327 GAGACC GGTCTC(1/5)
BsmBI 363 GAGACG CGTCTC(1/5)
BspDI 3143 ATCGAT AT^CGAT
BspLU11I 1699 ACATGT A^CATGT
BsrFI 1976 GCCGGT [GA]^CCGG[CT]
BstEII 3912 GGTCACC G^GT[ACGT]ACC
BstPI 3912 GGTCACC G^GT[ACGT]ACC
Cfr10I 1976 GCCGGT [GA]^CCGG[CT]
ClaI 3143 ATCGAT AT^CGAT
DraIII 441 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 5486 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 5090 CCTCTTATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3912 GGTCACC G^GT[ACGT]ACC
EcoRV 160 GATATC GAT^ATC
FauI 214 CCCGC CCCGC(4/6)
HpaI 5070 GTTAAC GTT^AAC
MluNI 864 TGGCCA TGG^CCA
MscI 864 TGGCCA TGG^CCA
PaeR7I 3603 CTCGAG C^TCGAG
PflFI 4834 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 3033 GTTTAAAC GTTT^AAAC
PshAI 3322 GACTCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3141 CGATCG CGAT^CG
ScaI 774 AGTACT AGT^ACT
SphI 2754 GCATGC GCATG^C
StuI 2539 AGGCCT AGG^CCT
Tth111I 4834 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3603 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AclI 4001 AACGTT AA^CGTT
5178 AACGTT
AcyI 166 GACGTC G[GA]^CG[CT]C
365 GACGCC
AflIII 1699 ACATGT A^C[GA][CT]GT
5697 ACACGT
BanI 1334 GGCACC G^G[CT][GA]CC
3573 GGTGCC
BciVI 1721 GGATAC GTATCC(6/5)
459 GTATCC
BsaHI 166 GACGTC G[GA]^CG[CT]C
365 GACGCC
BsiEI 345 CGGTCG CG[GA][CT]^CG
3141 CGATCG
BspHI 3168 TCATGA T^CATGA
4591 TCATGA
BspMI 293 ACCTGC ACCTGC(4/8)
873 ACCTGC
BsrBI 781 CCGCTC CCGCTC(-3/-3)
263 GAGCGG
BssSI 569 CTCGTG CACGAG(-5/-1)
3324 CTCGTG
BstXI 1222 CCAAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5301 CCACTACCATGG
DsaI 2942 CCATGG C^C[GA][CT]GG
5307 CCATGG
EaeI 22 CGGCCA [CT]^GGCC[GA]
864 TGGCCA
HgaI 365 GACGC GACGC(5/10)
2790 GCGTC
Hin1I 166 GACGTC G[GA]^CG[CT]C
365 GACGCC
HincII 4582 GTTGAC GT[CT]^[GA]AC
5070 GTTAAC
HindII 4582 GTTGAC GT[CT]^[GA]AC
5070 GTTAAC
NcoI 2942 CCATGG C^CATGG
5307 CCATGG
PpuMI 373 GGGTCCC [GA]G^G[AT]CC[CT]
574 GGGTCCC
Psp1406I 4001 AACGTT AA^CGTT
5178 AACGTT
RcaI 3168 TCATGA T^CATGA
4591 TCATGA
SmaI 406 CCCGGG CCC^GGG
530 CCCGGG
XmaCI 406 CCCGGG C^CCGGG
530 CCCGGG
XmaI 406 CCCGGG C^CCGGG
530 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI