################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII AccII AccIII AclI AflII AgeI AscI AseI AsnI AviII AvrII BbeI BfrI BlnI BseAI BsiWI BspDI BspEI BspLU11I BsrFI BssHII BstUI Cfr10I ClaI CpoI EcoRI FseI FspI HincII HindII HpaI KasI KspI MluI MroI MvnI NaeI NarI NgoMIV NheI NotI NruI PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SgrAI SnaBI SphI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 3492 GGTACC G^GTACC AcyI 6830 GACGCC G[GA]^CG[CT]C AflIII 4435 ACACGT A^C[GA][CT]GT AhdI 3785 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 3447 CCTGAGG CC^T[ACGT]AGG Aor51HI 2742 AGCGCT AGC^GCT Asp718I 3492 GGTACC G^GTACC AspEI 3785 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 4984 TGGCCA TGG^CCA BbrPI 2735 CACGTG CAC^GTG BbvCI 452 GCTGAGG CCTCAGC(-5/-2) BsaAI 2735 CACGTG [CT]AC^GT[GA] BsaHI 6830 GACGCC G[GA]^CG[CT]C BsaWI 6200 TCCGGT [AT]^CCGG[AT] BsiEI 4215 CGGCCG CG[GA][CT]^CG BssSI 2113 CTCGTG CACGAG(-5/-1) Bst1107I 122 GTATAC GTA^TAC BstEII 4878 GGTTACC G^GT[ACGT]ACC BstPI 4878 GGTTACC G^GT[ACGT]ACC BstXI 4786 CCACGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 122 GTATAC GTA^TAC Bsu36I 3447 CCTGAGG CC^T[ACGT]AGG DraIII 7063 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 4215 CGGCCG C^GGCCG Eam1105I 3785 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 4215 CGGCCG C^GGCCG Eco47III 2742 AGCGCT AGC^GCT Eco52I 4215 CGGCCG C^GGCCG Eco81I 3447 CCTGAGG CC^T[ACGT]AGG EcoO65I 4878 GGTTACC G^GT[ACGT]ACC EcoRV 5615 GATATC GAT^ATC HaeII 2742 AGCGCT [GA]GCGC^[CT] Hin1I 6830 GACGCC G[GA]^CG[CT]C KpnI 3492 GGTACC GGTAC^C MluNI 4984 TGGCCA TGG^CCA MscI 4984 TGGCCA TGG^CCA NspI 6939 GCATGT [GA]CATG^[CT] PaeR7I 2320 CTCGAG C^TCGAG PmaCI 2735 CACGTG CAC^GTG PmlI 2735 CACGTG CAC^GTG PshAI 3672 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 6918 AGTACT AGT^ACT SmaI 1750 CCCGGG CCC^GGG SpeI 303 ACTAGT A^CTAGT XbaI 6480 TCTAGA T^CTAGA XhoI 2320 CTCGAG C^TCGAG XmaCI 1750 CCCGGG C^CCGGG XmaI 1750 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 122 GTATAC GT^[AC][GT]AC 4625 GTCTAC Alw44I 432 GTGCAC G^TGCAC 4531 GTGCAC ApaI 3466 GGGCCC GGGCC^C 5495 GGGCCC ApaLI 432 GTGCAC G^TGCAC 4531 GTGCAC BcgI 230 GCAACAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1553 GCAAATTGCTCG BciVI 2913 GTATCC GTATCC(6/5) 4675 GTATCC BglII 1381 AGATCT A^GATCT 6436 AGATCT BsmBI 2837 CGTCTC CGTCTC(1/5) 6158 CGTCTC Bsp1407I 6800 TGTACA T^GTACA 7672 TGTACA BsrGI 6800 TGTACA T^GTACA 7672 TGTACA BstBI 238 TTCGAA TT^CGAA 1090 TTCGAA EcoT22I 2587 ATGCAT ATGCA^T 2991 ATGCAT HgaI 6830 GACGC GACGC(5/10) 4973 GCGTC MfeI 1990 CAATTG C^AATTG 2938 CAATTG MunI 1990 CAATTG C^AATTG 2938 CAATTG NcoI 5113 CCATGG C^CATGG 6343 CCATGG NdeI 3754 CATATG CA^TATG 4102 CATATG NsiI 2587 ATGCAT ATGCA^T 2991 ATGCAT NspV 238 TTCGAA TT^CGAA 1090 TTCGAA SapI 1857 GAAGAGC GCTCTTC(1/4) 2118 GAAGAGC SfuI 238 TTCGAA TT^CGAA 1090 TTCGAA SspBI 6800 TGTACA T^GTACA 7672 TGTACA SspI 3762 AATATT AAT^ATT 7770 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AspI AvaI AvaII BamHI BanI BanII BbsI BbvI BclI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DrdI DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflFI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmnI