##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AflII       
AgeI        AscI        AseI        AsnI        AviII       
AvrII       BbeI        BfrI        BlnI        BseAI       
BsiWI       BspDI       BspEI       BspLU11I    BsrFI       
BssHII      BstUI       Cfr10I      ClaI        CpoI        
EcoRI       FseI        FspI        HincII      HindII      
HpaI        KasI        KspI        MluI        MroI        
MvnI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        PacI        PinAI       PmeI        
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SbfI        SfiI        SfoI        
SgrAI       SnaBI       SphI        Sse8387I    SwaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I       3492  GGTACC               G^GTACC
AcyI         6830  GACGCC               G[GA]^CG[CT]C
AflIII       4435  ACACGT               A^C[GA][CT]GT
AhdI         3785  GACTAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         3447  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      2742  AGCGCT               AGC^GCT
Asp718I      3492  GGTACC               G^GTACC
AspEI        3785  GACTAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         4984  TGGCCA               TGG^CCA
BbrPI        2735  CACGTG               CAC^GTG
BbvCI         452  GCTGAGG              CCTCAGC(-5/-2)
BsaAI        2735  CACGTG               [CT]AC^GT[GA]
BsaHI        6830  GACGCC               G[GA]^CG[CT]C
BsaWI        6200  TCCGGT               [AT]^CCGG[AT]
BsiEI        4215  CGGCCG               CG[GA][CT]^CG
BssSI        2113  CTCGTG               CACGAG(-5/-1)
Bst1107I      122  GTATAC               GTA^TAC
BstEII       4878  GGTTACC              G^GT[ACGT]ACC
BstPI        4878  GGTTACC              G^GT[ACGT]ACC
BstXI        4786  CCACGCACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       122  GTATAC               GTA^TAC
Bsu36I       3447  CCTGAGG              CC^T[ACGT]AGG
DraIII       7063  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         4215  CGGCCG               C^GGCCG
Eam1105I     3785  GACTAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        4215  CGGCCG               C^GGCCG
Eco47III     2742  AGCGCT               AGC^GCT
Eco52I       4215  CGGCCG               C^GGCCG
Eco81I       3447  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      4878  GGTTACC              G^GT[ACGT]ACC
EcoRV        5615  GATATC               GAT^ATC
HaeII        2742  AGCGCT               [GA]GCGC^[CT]
Hin1I        6830  GACGCC               G[GA]^CG[CT]C
KpnI         3492  GGTACC               GGTAC^C
MluNI        4984  TGGCCA               TGG^CCA
MscI         4984  TGGCCA               TGG^CCA
NspI         6939  GCATGT               [GA]CATG^[CT]
PaeR7I       2320  CTCGAG               C^TCGAG
PmaCI        2735  CACGTG               CAC^GTG
PmlI         2735  CACGTG               CAC^GTG
PshAI        3672  GACAGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI         6918  AGTACT               AGT^ACT
SmaI         1750  CCCGGG               CCC^GGG
SpeI          303  ACTAGT               A^CTAGT
XbaI         6480  TCTAGA               T^CTAGA
XhoI         2320  CTCGAG               C^TCGAG
XmaCI        1750  CCCGGG               C^CCGGG
XmaI         1750  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI          122  GTATAC               GT^[AC][GT]AC
             4625  GTCTAC              
Alw44I        432  GTGCAC               G^TGCAC
             4531  GTGCAC              
ApaI         3466  GGGCCC               GGGCC^C
             5495  GGGCCC              
ApaLI         432  GTGCAC               G^TGCAC
             4531  GTGCAC              
BcgI          230  GCAACAGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1553  GCAAATTGCTCG        
BciVI        2913  GTATCC               GTATCC(6/5)
             4675  GTATCC              
BglII        1381  AGATCT               A^GATCT
             6436  AGATCT              
BsmBI        2837  CGTCTC               CGTCTC(1/5)
             6158  CGTCTC              
Bsp1407I     6800  TGTACA               T^GTACA
             7672  TGTACA              
BsrGI        6800  TGTACA               T^GTACA
             7672  TGTACA              
BstBI         238  TTCGAA               TT^CGAA
             1090  TTCGAA              
EcoT22I      2587  ATGCAT               ATGCA^T
             2991  ATGCAT              
HgaI         6830  GACGC                GACGC(5/10)
             4973  GCGTC               
MfeI         1990  CAATTG               C^AATTG
             2938  CAATTG              
MunI         1990  CAATTG               C^AATTG
             2938  CAATTG              
NcoI         5113  CCATGG               C^CATGG
             6343  CCATGG              
NdeI         3754  CATATG               CA^TATG
             4102  CATATG              
NsiI         2587  ATGCAT               ATGCA^T
             2991  ATGCAT              
NspV          238  TTCGAA               TT^CGAA
             1090  TTCGAA              
SapI         1857  GAAGAGC              GCTCTTC(1/4)
             2118  GAAGAGC             
SfuI          238  TTCGAA               TT^CGAA
             1090  TTCGAA              
SspBI        6800  TGTACA               T^GTACA
             7672  TGTACA              
SspI         3762  AATATT               AAT^ATT
             7770  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (  127 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AspI        
AvaI        AvaII       BamHI       BanI        BanII       
BbsI        BbvI        BclI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaBI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspHI       
BspMI       BsrBI       BsrDI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DrdI        DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflFI       PflMI       PleI        
PpuMI       PstI        PvuII       RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SexAI       SfaNI       
SfcI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoII       XmnI