################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AccIII AflII AgeI AocI AscI AseI AsnI BciVI BfrI BglII BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrGI BssHII BstBI BstEII BstPI Bsu36I ClaI CpoI DraI Eco81I EcoO65I EcoRV FseI HincII HindII HindIII HpaI KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AatII 1048 GACGTC GACGT^C AccI 731 GTATAC GT^[AC][GT]AC AclI 2731 AACGTT AA^CGTT AflIII 2435 ACATGT A^C[GA][CT]GT AhdI 2238 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 2238 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 5562 CCTAGG C^CTAGG BamHI 807 GGATCC G^GATCC BbeI 5046 GGCGCC GGCGC^C BcgI 1561 CGAAAGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 5562 CCTAGG C^CTAGG BsaWI 31 ACCGGA [AT]^CCGG[AT] BsmI 2346 GCATTC GAATGC(1/-1) BspLU11I 2435 ACATGT A^CATGT BsrFI 1447 GCCGGT [GA]^CCGG[CT] BssSI 410 CTCGTG CACGAG(-5/-1) Bst1107I 731 GTATAC GTA^TAC BstZ17I 731 GTATAC GTA^TAC Cfr10I 1447 GCCGGT [GA]^CCGG[CT] DrdI 1549 GACAGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 278 CGGCCG C^GGCCG Eam1105I 2238 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 278 CGGCCG C^GGCCG Eco52I 278 CGGCCG C^GGCCG EcoRI 1531 GAATTC G^AATTC EcoT22I 757 ATGCAT ATGCA^T KasI 5046 GGCGCC G^GCGCC NarI 5046 GGCGCC GG^CGCC NheI 3697 GCTAGC G^CTAGC NsiI 757 ATGCAT ATGCA^T NspI 2435 ACATGT [GA]CATG^[CT] PshAI 4411 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2731 AACGTT AA^CGTT SacI 1216 GAGCTC GAGCT^C SfoI 5046 GGCGCC GGC^GCC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Acc65I 864 GGTACC G^GTACC 3660 GGTACC Aor51HI 1203 AGCGCT AGC^GCT 1287 AGCGCT Asp700I 1201 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1285 GAAGCGCTTC Asp718I 864 GGTACC G^GTACC 3660 GGTACC AviII 288 TGCGCA TGC^GCA 4180 TGCGCA BglI 4387 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5516 GCCAGGCTGGC BsiEI 278 CGGCCG CG[GA][CT]^CG 1129 CGACCG BsmBI 158 GAGACG CGTCTC(1/5) 1952 GAGACG BstAPI 1272 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1652 GCACTGGGTGC DraIII 1273 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG 1653 CACTGGGTG Eco47III 1203 AGCGCT AGC^GCT 1287 AGCGCT FspI 288 TGCGCA TGC^GCA 4180 TGCGCA KpnI 864 GGTACC GGTAC^C 3660 GGTACC MslI 1331 CACACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3931 CACTCACGTG NcoI 994 CCATGG C^CATGG 1590 CCATGG SmaI 231 CCCGGG CCC^GGG 2332 CCCGGG TaqI 562 TCGA T^CGA 3152 TCGA TthHB8I 562 TCGA T^CGA 3152 TCGA XbaI 3625 TCTAGA T^CTAGA 5400 TCTAGA XmaCI 231 CCCGGG C^CCGGG 2332 CCCGGG XmaI 231 CCCGGG C^CCGGG 2332 CCCGGG XmnI 1201 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1285 GAAGCGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI ApaI ApaLI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvCI BbvI BclI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflFI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I Van91I XcmI XhoII