##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AatII       AflII       AgeI        AhdI        AscI        
AseI        AsnI        AspEI       BamHI       BbrPI       
BcgI        BciVI       BfrI        BglII       BsiWI       
BsmI        Bsp1407I    BspDI       BspHI       BsrGI       
BssHII      BstBI       ClaI        CpoI        DrdI        
EagI        Eam1105I    EclXI       Eco52I      EcoRV       
EcoT22I     FseI        HpaI        MfeI        MluI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       PvuI        
RcaI        RsrII       SalI        SapI        SbfI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

Acc65I       5240  GGTACC               G^GTACC
AccI         4389  GTATAC               GT^[AC][GT]AC
AclI         4563  AACGTT               AA^CGTT
Asp718I      5240  GGTACC               G^GTACC
AvrII        2713  CCTAGG               C^CTAGG
BbeI          114  GGCGCC               GGCGC^C
BbvCI        5435  CCTCAGC              CCTCAGC(-5/-2)
BclI         2842  TGATCA               T^GATCA
BlnI         2713  CCTAGG               C^CTAGG
BlpI          974  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      974  GCTCAGC              GC^T[ACGT]AGC
BsaAI         376  TACGTG               [CT]AC^GT[GA]
BsgI          394  GTGCAG               GTGCAG(16/14)
BspLU11I     4417  ACATGT               A^CATGT
Bst1107I     4389  GTATAC               GTA^TAC
BstZ17I      4389  GTATAC               GTA^TAC
CelII         974  GCTCAGC              GC^T[ACGT]AGC
DraIII       4245  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRI        3298  GAATTC               G^AATTC
FbaI         2842  TGATCA               T^GATCA
HincII       4745  GTTGAC               GT[CT]^[GA]AC
HindII       4745  GTTGAC               GT[CT]^[GA]AC
KasI          114  GGCGCC               G^GCGCC
KpnI         5240  GGTACC               GGTAC^C
NarI          114  GGCGCC               GG^CGCC
Psp1406I     4563  AACGTT               AA^CGTT
ScaI         2045  AGTACT               AGT^ACT
SfoI          114  GGCGCC               GGC^GCC


###################################
# TWO CUT ENZYMES (   43 enzymes) #
###################################

AccIII        170  TCCGGA               T^CCGGA
             1628  TCCGGA              
AcyI          114  GGCGCC               G[GA]^CG[CT]C
             2028  GACGCC              
AflIII       2569  ACGTGT               A^C[GA][CT]GT
             4417  ACATGT              
Alw44I       2128  GTGCAC               G^TGCAC
             5055  GTGCAC              
ApaI         2120  GGGCCC               GGGCC^C
             5277  GGGCCC              
ApaLI        2128  GTGCAC               G^TGCAC
             5055  GTGCAC              
Asp700I       837  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1656  GAAGGACTTC          
AspI          444  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1971  GACCCGGTC           
AviII        1526  TGCGCA               TGC^GCA
             1748  TGCGCA              
BbsI         1317  GTCTTC               GAAGAC(2/6)
             1735  GTCTTC              
BpuAI        1317  GTCTTC               GAAGAC(2/6)
             1735  GTCTTC              
BsaBI        1410  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4861  GATGTTGATC          
BsaHI         114  GGCGCC               G[GA]^CG[CT]C
             2028  GACGCC              
BsaWI         170  TCCGGA               [AT]^CCGG[AT]
             1628  TCCGGA              
BseAI         170  TCCGGA               T^CCGGA
             1628  TCCGGA              
BsmBI        1372  GAGACG               CGTCTC(1/5)
             2509  GAGACG              
BspEI         170  TCCGGA               T^CCGGA
             1628  TCCGGA              
BsrDI        4804  CATTGC               GCAATG(2/0)
             1091  GCAATG              
BssSI        1780  CTCGTG               CACGAG(-5/-1)
             3638  CTCGTG              
BstAPI        396  GCAGGGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              687  GCAGCCCCTGC         
BstEII        913  GGTGACC              G^GT[ACGT]ACC
             3328  GGTGACC             
BstPI         913  GGTGACC              G^GT[ACGT]ACC
             3328  GGTGACC             
BstXI         903  CCAACAGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1466  CCACCTTCATGG        
BstYI        1415  AGATCC               [GA]^GATC[CT]
             2410  GGATCT              
DraI         4583  TTTAAA               TTT^AAA
             5707  TTTAAA              
EarI         4132  GAAGAG               CTCTTC(1/4)
             4840  GAAGAG              
EcoO65I       913  GGTGACC              G^GT[ACGT]ACC
             3328  GGTGACC             
FspI         1526  TGCGCA               TGC^GCA
             1748  TGCGCA              
Hin1I         114  GGCGCC               G[GA]^CG[CT]C
             2028  GACGCC              
HindIII      3352  AAGCTT               A^AGCTT
             4220  AAGCTT              
Ksp632I      4132  GAAGAG               CTCTTC(1/4)
             4840  GAAGAG              
MamI         1410  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4861  GATGTTGATC          
MflI         1415  AGATCC               [GA]^GATC[CT]
             2410  GGATCT              
MroI          170  TCCGGA               T^CCGGA
             1628  TCCGGA              
NaeI         1880  GCCGGC               GCC^GGC
             5560  GCCGGC              
NgoMIV       1880  GCCGGC               G^CCGGC
             5560  GCCGGC              
PflFI         444  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1971  GACCCGGTC           
SexAI         389  ACCAGGT              A^CC[AT]GGT
             3074  ACCTGGT             
SfiI         3084  GGCCCCAGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             3557  GGCCGGGCAGGCC       
Tth111I       444  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1971  GACCCGGTC           
XbaI         2904  TCTAGA               T^CTAGA
             3145  TCTAGA              
XhoII        1415  AGATCC               [GA]^GATC[CT]
             2410  GGATCT              
XmnI          837  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1656  GAAGGACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       AocI        Aor51HI     ApoI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaI        BsaJI       BseRI       
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        Eco47III    Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
KspI        MaeI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PaeR7I      PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SphI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoI