################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII AflII AgeI AhdI AscI AseI AsnI AspEI BamHI BbrPI BcgI BciVI BfrI BglII BsiWI BsmI Bsp1407I BspDI BspHI BsrGI BssHII BstBI ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FseI HpaI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SalI SapI SbfI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### Acc65I 5240 GGTACC G^GTACC AccI 4389 GTATAC GT^[AC][GT]AC AclI 4563 AACGTT AA^CGTT Asp718I 5240 GGTACC G^GTACC AvrII 2713 CCTAGG C^CTAGG BbeI 114 GGCGCC GGCGC^C BbvCI 5435 CCTCAGC CCTCAGC(-5/-2) BclI 2842 TGATCA T^GATCA BlnI 2713 CCTAGG C^CTAGG BlpI 974 GCTCAGC GC^T[ACGT]AGC Bpu1102I 974 GCTCAGC GC^T[ACGT]AGC BsaAI 376 TACGTG [CT]AC^GT[GA] BsgI 394 GTGCAG GTGCAG(16/14) BspLU11I 4417 ACATGT A^CATGT Bst1107I 4389 GTATAC GTA^TAC BstZ17I 4389 GTATAC GTA^TAC CelII 974 GCTCAGC GC^T[ACGT]AGC DraIII 4245 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 3298 GAATTC G^AATTC FbaI 2842 TGATCA T^GATCA HincII 4745 GTTGAC GT[CT]^[GA]AC HindII 4745 GTTGAC GT[CT]^[GA]AC KasI 114 GGCGCC G^GCGCC KpnI 5240 GGTACC GGTAC^C NarI 114 GGCGCC GG^CGCC Psp1406I 4563 AACGTT AA^CGTT ScaI 2045 AGTACT AGT^ACT SfoI 114 GGCGCC GGC^GCC ################################### # TWO CUT ENZYMES ( 43 enzymes) # ################################### AccIII 170 TCCGGA T^CCGGA 1628 TCCGGA AcyI 114 GGCGCC G[GA]^CG[CT]C 2028 GACGCC AflIII 2569 ACGTGT A^C[GA][CT]GT 4417 ACATGT Alw44I 2128 GTGCAC G^TGCAC 5055 GTGCAC ApaI 2120 GGGCCC GGGCC^C 5277 GGGCCC ApaLI 2128 GTGCAC G^TGCAC 5055 GTGCAC Asp700I 837 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1656 GAAGGACTTC AspI 444 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1971 GACCCGGTC AviII 1526 TGCGCA TGC^GCA 1748 TGCGCA BbsI 1317 GTCTTC GAAGAC(2/6) 1735 GTCTTC BpuAI 1317 GTCTTC GAAGAC(2/6) 1735 GTCTTC BsaBI 1410 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4861 GATGTTGATC BsaHI 114 GGCGCC G[GA]^CG[CT]C 2028 GACGCC BsaWI 170 TCCGGA [AT]^CCGG[AT] 1628 TCCGGA BseAI 170 TCCGGA T^CCGGA 1628 TCCGGA BsmBI 1372 GAGACG CGTCTC(1/5) 2509 GAGACG BspEI 170 TCCGGA T^CCGGA 1628 TCCGGA BsrDI 4804 CATTGC GCAATG(2/0) 1091 GCAATG BssSI 1780 CTCGTG CACGAG(-5/-1) 3638 CTCGTG BstAPI 396 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 687 GCAGCCCCTGC BstEII 913 GGTGACC G^GT[ACGT]ACC 3328 GGTGACC BstPI 913 GGTGACC G^GT[ACGT]ACC 3328 GGTGACC BstXI 903 CCAACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1466 CCACCTTCATGG BstYI 1415 AGATCC [GA]^GATC[CT] 2410 GGATCT DraI 4583 TTTAAA TTT^AAA 5707 TTTAAA EarI 4132 GAAGAG CTCTTC(1/4) 4840 GAAGAG EcoO65I 913 GGTGACC G^GT[ACGT]ACC 3328 GGTGACC FspI 1526 TGCGCA TGC^GCA 1748 TGCGCA Hin1I 114 GGCGCC G[GA]^CG[CT]C 2028 GACGCC HindIII 3352 AAGCTT A^AGCTT 4220 AAGCTT Ksp632I 4132 GAAGAG CTCTTC(1/4) 4840 GAAGAG MamI 1410 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4861 GATGTTGATC MflI 1415 AGATCC [GA]^GATC[CT] 2410 GGATCT MroI 170 TCCGGA T^CCGGA 1628 TCCGGA NaeI 1880 GCCGGC GCC^GGC 5560 GCCGGC NgoMIV 1880 GCCGGC G^CCGGC 5560 GCCGGC PflFI 444 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1971 GACCCGGTC SexAI 389 ACCAGGT A^CC[AT]GGT 3074 ACCTGGT SfiI 3084 GGCCCCAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 3557 GGCCGGGCAGGCC Tth111I 444 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1971 GACCCGGTC XbaI 2904 TCTAGA T^CTAGA 3145 TCTAGA XhoII 1415 AGATCC [GA]^GATC[CT] 2410 GGATCT XmnI 837 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1656 GAAGGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI AocI Aor51HI ApoI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI KspI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PaeR7I PflMI PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoI