################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## Acc65I AccIII AflII AgeI ApaI AscI AseI AsnI Asp718I BcgI BciVI BfrI BglI BseAI BsiEI BsiWI BspDI BspEI BsrBI BsrFI BssHII Bst1107I BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraI EagI EclXI Eco52I EcoO65I FseI KpnI KspI MluI MroI NaeI NcoI NgoMIV NheI NotI NruI PacI PinAI PmeI PpuMI PshAI PshBI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SgrAI SmaI SpeI Sse8387I SwaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 2956 GTCTAC GT^[AC][GT]AC AccII 808 CGCG CG^CG AhdI 743 GACTCTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1274 AGCGCT AGC^GCT AspEI 743 GACTCTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 309 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 840 TGCGCA TGC^GCA BamHI 1229 GGATCC G^GATCC BbeI 5337 GGCGCC GGCGC^C BclI 2650 TGATCA T^GATCA BcnI 5276 CCGGG CC^[GC]GG BsaBI 186 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1153 ACCGGA [AT]^CCGG[AT] BsmBI 3661 CGTCTC CGTCTC(1/5) BstAPI 1941 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 808 CGCG CG^CG DraIII 4448 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 9 GACCAGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 5190 CCGTGG C^C[GA][CT]GG Eam1105I 743 GACTCTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1274 AGCGCT AGC^GCT EcoT22I 5730 ATGCAT ATGCA^T FbaI 2650 TGATCA T^GATCA FspI 840 TGCGCA TGC^GCA HpaI 5776 GTTAAC GTT^AAC KasI 5337 GGCGCC G^GCGCC MamI 186 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 5565 CAATTG C^AATTG MunI 5565 CAATTG C^AATTG MvnI 808 CGCG CG^CG NarI 5337 GGCGCC GG^CGCC NciI 5276 CCGGG CC^[GC]GG NdeI 2588 CATATG CA^TATG NsiI 5730 ATGCAT ATGCA^T PaeR7I 1038 CTCGAG C^TCGAG PflFI 309 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC SfoI 5337 GGCGCC GGC^GCC Tth111I 309 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1038 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AatII 312 GACGTC GACGT^C 1287 GACGTC AclI 1714 AACGTT AA^CGTT 3881 AACGTT Alw44I 501 GTGCAC G^TGCAC 2907 GTGCAC AlwNI 409 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG 438 CAGGGGCTG ApaLI 501 GTGCAC G^TGCAC 2907 GTGCAC AvaII 621 GGACC G^G[AT]CC 3432 GGTCC AvrII 2194 CCTAGG C^CTAGG 4245 CCTAGG BalI 693 TGGCCA TGG^CCA 5055 TGGCCA BbvCI 1991 CCTCAGC CCTCAGC(-5/-2) 5367 GCTGAGG BglII 3688 AGATCT A^GATCT 5600 AGATCT BlnI 2194 CCTAGG C^CTAGG 4245 CCTAGG BlpI 1978 GCTCAGC GC^T[ACGT]AGC 2987 GCTCAGC Bpu1102I 1978 GCTCAGC GC^T[ACGT]AGC 2987 GCTCAGC Bsp1407I 3807 TGTACA T^GTACA 5742 TGTACA BspHI 3617 TCATGA T^CATGA 4483 TCATGA BsrGI 3807 TGTACA T^GTACA 5742 TGTACA BssSI 367 CACGAG CACGAG(-5/-1) 2232 CTCGTG BstBI 1504 TTCGAA TT^CGAA 3494 TTCGAA CelII 1978 GCTCAGC GC^T[ACGT]AGC 2987 GCTCAGC EcoNI 174 CCTGATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1025 CCTTCATAAGG EcoRV 589 GATATC GAT^ATC 3187 GATATC EcoT14I 2194 CCTAGG C^C[AT][AT]GG 4245 CCTAGG FauI 1837 CCCGC CCCGC(4/6) 5234 GCGGG HaeII 1274 AGCGCT [GA]GCGC^[CT] 5337 GGCGCC HapII 1154 CCGG C^CGG 5276 CCGG HgaI 2313 GCGTC GACGC(5/10) 4028 GCGTC HpaII 1154 CCGG C^CGG 5276 CCGG MluNI 693 TGGCCA TGG^CCA 5055 TGGCCA MscI 693 TGGCCA TGG^CCA 5055 TGGCCA MspI 1154 CCGG C^CGG 5276 CCGG NspV 1504 TTCGAA TT^CGAA 3494 TTCGAA Psp1406I 1714 AACGTT AA^CGTT 3881 AACGTT PstI 2365 CTGCAG CTGCA^G 3448 CTGCAG RcaI 3617 TCATGA T^CATGA 4483 TCATGA SfuI 1504 TTCGAA TT^CGAA 3494 TTCGAA SnaBI 1252 TACGTA TAC^GTA 3093 TACGTA SphI 645 GCATGC GCATG^C 5666 GCATGC SspBI 3807 TGTACA T^GTACA 5742 TGTACA StyI 2194 CCTAGG C^C[AT][AT]GG 4245 CCTAGG XbaI 5128 TCTAGA T^CTAGA 5800 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI AocI ApoI Asp700I AspHI AvaI BanI BanII BbrPI BbsI BbvI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoO109I EcoRI EcoRII Fnu4HI FokI HaeIII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StuI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI