##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

Acc65I      AccIII      AflII       AgeI        ApaI        
AscI        AseI        AsnI        Asp718I     BcgI        
BciVI       BfrI        BglI        BseAI       BsiEI       
BsiWI       BspDI       BspEI       BsrBI       BsrFI       
BssHII      Bst1107I    BstEII      BstPI       BstZ17I     
Cfr10I      ClaI        CpoI        DraI        EagI        
EclXI       Eco52I      EcoO65I     FseI        KpnI        
KspI        MluI        MroI        NaeI        NcoI        
NgoMIV      NheI        NotI        NruI        PacI        
PinAI       PmeI        PpuMI       PshAI       PshBI       
PvuI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SgrAI       
SmaI        SpeI        Sse8387I    SwaI        XcmI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI         2956  GTCTAC               GT^[AC][GT]AC
AccII         808  CGCG                 CG^CG
AhdI          743  GACTCTACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      1274  AGCGCT               AGC^GCT
AspEI         743  GACTCTACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          309  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         840  TGCGCA               TGC^GCA
BamHI        1229  GGATCC               G^GATCC
BbeI         5337  GGCGCC               GGCGC^C
BclI         2650  TGATCA               T^GATCA
BcnI         5276  CCGGG                CC^[GC]GG
BsaBI         186  GATCCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1153  ACCGGA               [AT]^CCGG[AT]
BsmBI        3661  CGTCTC               CGTCTC(1/5)
BstAPI       1941  GCAGTGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         808  CGCG                 CG^CG
DraIII       4448  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI            9  GACCAGTCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         5190  CCGTGG               C^C[GA][CT]GG
Eam1105I      743  GACTCTACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1274  AGCGCT               AGC^GCT
EcoT22I      5730  ATGCAT               ATGCA^T
FbaI         2650  TGATCA               T^GATCA
FspI          840  TGCGCA               TGC^GCA
HpaI         5776  GTTAAC               GTT^AAC
KasI         5337  GGCGCC               G^GCGCC
MamI          186  GATCCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         5565  CAATTG               C^AATTG
MunI         5565  CAATTG               C^AATTG
MvnI          808  CGCG                 CG^CG
NarI         5337  GGCGCC               GG^CGCC
NciI         5276  CCGGG                CC^[GC]GG
NdeI         2588  CATATG               CA^TATG
NsiI         5730  ATGCAT               ATGCA^T
PaeR7I       1038  CTCGAG               C^TCGAG
PflFI         309  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SfoI         5337  GGCGCC               GGC^GCC
Tth111I       309  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1038  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   40 enzymes) #
###################################

AatII         312  GACGTC               GACGT^C
             1287  GACGTC              
AclI         1714  AACGTT               AA^CGTT
             3881  AACGTT              
Alw44I        501  GTGCAC               G^TGCAC
             2907  GTGCAC              
AlwNI         409  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              438  CAGGGGCTG           
ApaLI         501  GTGCAC               G^TGCAC
             2907  GTGCAC              
AvaII         621  GGACC                G^G[AT]CC
             3432  GGTCC               
AvrII        2194  CCTAGG               C^CTAGG
             4245  CCTAGG              
BalI          693  TGGCCA               TGG^CCA
             5055  TGGCCA              
BbvCI        1991  CCTCAGC              CCTCAGC(-5/-2)
             5367  GCTGAGG             
BglII        3688  AGATCT               A^GATCT
             5600  AGATCT              
BlnI         2194  CCTAGG               C^CTAGG
             4245  CCTAGG              
BlpI         1978  GCTCAGC              GC^T[ACGT]AGC
             2987  GCTCAGC             
Bpu1102I     1978  GCTCAGC              GC^T[ACGT]AGC
             2987  GCTCAGC             
Bsp1407I     3807  TGTACA               T^GTACA
             5742  TGTACA              
BspHI        3617  TCATGA               T^CATGA
             4483  TCATGA              
BsrGI        3807  TGTACA               T^GTACA
             5742  TGTACA              
BssSI         367  CACGAG               CACGAG(-5/-1)
             2232  CTCGTG              
BstBI        1504  TTCGAA               TT^CGAA
             3494  TTCGAA              
CelII        1978  GCTCAGC              GC^T[ACGT]AGC
             2987  GCTCAGC             
EcoNI         174  CCTGATTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1025  CCTTCATAAGG         
EcoRV         589  GATATC               GAT^ATC
             3187  GATATC              
EcoT14I      2194  CCTAGG               C^C[AT][AT]GG
             4245  CCTAGG              
FauI         1837  CCCGC                CCCGC(4/6)
             5234  GCGGG               
HaeII        1274  AGCGCT               [GA]GCGC^[CT]
             5337  GGCGCC              
HapII        1154  CCGG                 C^CGG
             5276  CCGG                
HgaI         2313  GCGTC                GACGC(5/10)
             4028  GCGTC               
HpaII        1154  CCGG                 C^CGG
             5276  CCGG                
MluNI         693  TGGCCA               TGG^CCA
             5055  TGGCCA              
MscI          693  TGGCCA               TGG^CCA
             5055  TGGCCA              
MspI         1154  CCGG                 C^CGG
             5276  CCGG                
NspV         1504  TTCGAA               TT^CGAA
             3494  TTCGAA              
Psp1406I     1714  AACGTT               AA^CGTT
             3881  AACGTT              
PstI         2365  CTGCAG               CTGCA^G
             3448  CTGCAG              
RcaI         3617  TCATGA               T^CATGA
             4483  TCATGA              
SfuI         1504  TTCGAA               TT^CGAA
             3494  TTCGAA              
SnaBI        1252  TACGTA               TAC^GTA
             3093  TACGTA              
SphI          645  GCATGC               GCATG^C
             5666  GCATGC              
SspBI        3807  TGTACA               T^GTACA
             5742  TGTACA              
StyI         2194  CCTAGG               C^C[AT][AT]GG
             4245  CCTAGG              
XbaI         5128  TCTAGA               T^CTAGA
             5800  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AflIII      
AluI        AlwI        AocI        ApoI        Asp700I     
AspHI       AvaI        BanI        BanII       BbrPI       
BbsI        BbvI        BfaI        BmyI        BpmI        
BpuAI       BsaAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspLU11I    BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        Eco81I      EcoO109I    
EcoRI       EcoRII      Fnu4HI      FokI        HaeIII      
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PmaCI       PmlI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspI        
StuI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       XmnI