##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccIII AflII AgeI ApaI
AscI AseI AsnI Asp718I BcgI
BciVI BfrI BglI BseAI BsiEI
BsiWI BspDI BspEI BsrBI BsrFI
BssHII Bst1107I BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraI EagI
EclXI Eco52I EcoO65I FseI KpnI
KspI MluI MroI NaeI NcoI
NgoMIV NheI NotI NruI PacI
PinAI PmeI PpuMI PshAI PshBI
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SgrAI
SmaI SpeI Sse8387I SwaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 2956 GTCTAC GT^[AC][GT]AC
AccII 808 CGCG CG^CG
AhdI 743 GACTCTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1274 AGCGCT AGC^GCT
AspEI 743 GACTCTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 309 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 840 TGCGCA TGC^GCA
BamHI 1229 GGATCC G^GATCC
BbeI 5337 GGCGCC GGCGC^C
BclI 2650 TGATCA T^GATCA
BcnI 5276 CCGGG CC^[GC]GG
BsaBI 186 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1153 ACCGGA [AT]^CCGG[AT]
BsmBI 3661 CGTCTC CGTCTC(1/5)
BstAPI 1941 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 808 CGCG CG^CG
DraIII 4448 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 9 GACCAGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 5190 CCGTGG C^C[GA][CT]GG
Eam1105I 743 GACTCTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1274 AGCGCT AGC^GCT
EcoT22I 5730 ATGCAT ATGCA^T
FbaI 2650 TGATCA T^GATCA
FspI 840 TGCGCA TGC^GCA
HpaI 5776 GTTAAC GTT^AAC
KasI 5337 GGCGCC G^GCGCC
MamI 186 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5565 CAATTG C^AATTG
MunI 5565 CAATTG C^AATTG
MvnI 808 CGCG CG^CG
NarI 5337 GGCGCC GG^CGCC
NciI 5276 CCGGG CC^[GC]GG
NdeI 2588 CATATG CA^TATG
NsiI 5730 ATGCAT ATGCA^T
PaeR7I 1038 CTCGAG C^TCGAG
PflFI 309 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfoI 5337 GGCGCC GGC^GCC
Tth111I 309 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1038 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AatII 312 GACGTC GACGT^C
1287 GACGTC
AclI 1714 AACGTT AA^CGTT
3881 AACGTT
Alw44I 501 GTGCAC G^TGCAC
2907 GTGCAC
AlwNI 409 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
438 CAGGGGCTG
ApaLI 501 GTGCAC G^TGCAC
2907 GTGCAC
AvaII 621 GGACC G^G[AT]CC
3432 GGTCC
AvrII 2194 CCTAGG C^CTAGG
4245 CCTAGG
BalI 693 TGGCCA TGG^CCA
5055 TGGCCA
BbvCI 1991 CCTCAGC CCTCAGC(-5/-2)
5367 GCTGAGG
BglII 3688 AGATCT A^GATCT
5600 AGATCT
BlnI 2194 CCTAGG C^CTAGG
4245 CCTAGG
BlpI 1978 GCTCAGC GC^T[ACGT]AGC
2987 GCTCAGC
Bpu1102I 1978 GCTCAGC GC^T[ACGT]AGC
2987 GCTCAGC
Bsp1407I 3807 TGTACA T^GTACA
5742 TGTACA
BspHI 3617 TCATGA T^CATGA
4483 TCATGA
BsrGI 3807 TGTACA T^GTACA
5742 TGTACA
BssSI 367 CACGAG CACGAG(-5/-1)
2232 CTCGTG
BstBI 1504 TTCGAA TT^CGAA
3494 TTCGAA
CelII 1978 GCTCAGC GC^T[ACGT]AGC
2987 GCTCAGC
EcoNI 174 CCTGATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1025 CCTTCATAAGG
EcoRV 589 GATATC GAT^ATC
3187 GATATC
EcoT14I 2194 CCTAGG C^C[AT][AT]GG
4245 CCTAGG
FauI 1837 CCCGC CCCGC(4/6)
5234 GCGGG
HaeII 1274 AGCGCT [GA]GCGC^[CT]
5337 GGCGCC
HapII 1154 CCGG C^CGG
5276 CCGG
HgaI 2313 GCGTC GACGC(5/10)
4028 GCGTC
HpaII 1154 CCGG C^CGG
5276 CCGG
MluNI 693 TGGCCA TGG^CCA
5055 TGGCCA
MscI 693 TGGCCA TGG^CCA
5055 TGGCCA
MspI 1154 CCGG C^CGG
5276 CCGG
NspV 1504 TTCGAA TT^CGAA
3494 TTCGAA
Psp1406I 1714 AACGTT AA^CGTT
3881 AACGTT
PstI 2365 CTGCAG CTGCA^G
3448 CTGCAG
RcaI 3617 TCATGA T^CATGA
4483 TCATGA
SfuI 1504 TTCGAA TT^CGAA
3494 TTCGAA
SnaBI 1252 TACGTA TAC^GTA
3093 TACGTA
SphI 645 GCATGC GCATG^C
5666 GCATGC
SspBI 3807 TGTACA T^GTACA
5742 TGTACA
StyI 2194 CCTAGG C^C[AT][AT]GG
4245 CCTAGG
XbaI 5128 TCTAGA T^CTAGA
5800 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AocI ApoI Asp700I
AspHI AvaI BanI BanII BbrPI
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI Eco81I EcoO109I
EcoRI EcoRII Fnu4HI FokI HaeIII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI