##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AccIII AcsI AflII AgeI
Aor51HI ApoI AscI Asp718I BamHI
BbrPI BciVI BfrI BglII BsaBI
BseAI BsiWI BspDI BspEI BspLU11I
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI Eco47III EcoO65I
EcoRI EcoRV FseI HindIII KpnI
MamI MluI MroI NdeI NotI
NruI NspV PaeR7I PinAI PmaCI
PmeI PmlI PvuI RsrII SfiI
SfuI SgrAI SnaBI SphI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 242 GTCGAC GT^[AC][GT]AC
AclI 1822 AACGTT AA^CGTT
AhdI 956 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4884 GTGCAC G^TGCAC
AocI 559 CCTAAGG CC^T[ACGT]AGG
ApaLI 4884 GTGCAC G^TGCAC
AspEI 956 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1050 TGCGCA TGC^GCA
AvrII 2473 CCTAGG C^CTAGG
BcgI 5082 CGACCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1185 TGATCA T^GATCA
BlnI 2473 CCTAGG C^CTAGG
BsiEI 3395 CGGCCG CG[GA][CT]^CG
BsmI 2065 GAATGC GAATGC(1/-1)
BsrBI 907 GAGCGG CCGCTC(-3/-3)
BsrDI 2035 CATTGC GCAATG(2/0)
BssHII 56 GCGCGC G^CGCGC
Bsu36I 559 CCTAAGG CC^T[ACGT]AGG
DraI 4996 TTTAAA TTT^AAA
DrdI 5190 GACAAAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3395 CGGCCG C^GGCCG
Eam1105I 956 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3395 CGGCCG C^GGCCG
Eco52I 3395 CGGCCG C^GGCCG
Eco81I 559 CCTAAGG CC^T[ACGT]AGG
EcoT22I 4780 ATGCAT ATGCA^T
FbaI 1185 TGATCA T^GATCA
FspI 1050 TGCGCA TGC^GCA
HpaI 2627 GTTAAC GTT^AAC
MfeI 1243 CAATTG C^AATTG
MunI 1243 CAATTG C^AATTG
NheI 2551 GCTAGC G^CTAGC
NsiI 4780 ATGCAT ATGCA^T
NspI 4404 ACATGC [GA]CATG^[CT]
PshAI 934 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1822 AACGTT AA^CGTT
SalI 242 GTCGAC G^TCGAC
SapI 4656 GCTCTTC GCTCTTC(1/4)
SbfI 1972 CCTGCAGG CCTGCA^GG
SmaI 2847 CCCGGG CCC^GGG
SpeI 4378 ACTAGT A^CTAGT
Sse8387I 1972 CCTGCAGG CCTGCA^GG
SspI 5242 AATATT AAT^ATT
XbaI 1396 TCTAGA T^CTAGA
XmaCI 2847 CCCGGG C^CCGGG
XmaI 2847 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
ApaI 116 GGGCCC GGGCC^C
219 GGGCCC
AspI 872 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4473 GACTTTGTC
BlpI 503 GCTGAGC GC^T[ACGT]AGC
2467 GCTCAGC
Bpu1102I 503 GCTGAGC GC^T[ACGT]AGC
2467 GCTCAGC
BsaAI 1670 TACGTG [CT]AC^GT[GA]
2233 CACGTA
BseRI 3374 CTCCTC GAGGAG(10/8)
4764 GAGGAG
BsmBI 2999 GAGACG CGTCTC(1/5)
3200 GAGACG
Bsp1407I 622 TGTACA T^GTACA
5162 TGTACA
BspHI 1188 TCATGA T^CATGA
3021 TCATGA
BsrGI 622 TGTACA T^GTACA
5162 TGTACA
CelII 503 GCTGAGC GC^T[ACGT]AGC
2467 GCTCAGC
KspI 2976 CCGCGG CCGC^GG
3319 CCGCGG
NcoI 1416 CCATGG C^CATGG
3936 CCATGG
PacI 4785 TTAATTAA TTAAT^TAA
4801 TTAATTAA
PflFI 872 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4473 GACTTTGTC
PflMI 786 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4273 CCAAATGCTGG
RcaI 1188 TCATGA T^CATGA
3021 TCATGA
SacII 2976 CCGCGG CCGC^GG
3319 CCGCGG
ScaI 2049 AGTACT AGT^ACT
3036 AGTACT
SmlI 1691 CTCAAG C^T[CT][GA]AG
3405 CTCAAG
SspBI 622 TGTACA T^GTACA
5162 TGTACA
TfiI 2670 GATTC G^A[AT]TC
5230 GATTC
Tth111I 872 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4473 GACTTTGTC
Van91I 786 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4273 CCAAATGCTGG
XcmI 2739 CCAACCTGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3169 CCAGTGCGAGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AseI
AsnI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PshBI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfoI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI