##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AgeI        Aor51HI     AscI        Asp718I     AspI        
AvrII       BbeI        BbrPI       BbvCI       BcgI        
BclI        BglII       BlnI        BsaHI       BsaWI       
BseAI       BsiWI       BsmBI       BspDI       BspEI       
BspHI       BsrBI       BssHII      BssSI       BstEII      
BstPI       ClaI        CpoI        DraIII      DrdI        
Eco47III    EcoO65I     EcoRV       FbaI        HgaI        
Hin1I       KasI        KpnI        KspI        MluI        
MroI        NarI        NdeI        NotI        NruI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SgrAI       
SmaI        Sse8387I    SwaI        Tth111I     XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccII         944  CGCG                 CG^CG
AhdI         4878  GACACTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1298  GTGCAC               G^TGCAC
ApaLI        1298  GTGCAC               G^TGCAC
AseI         3882  ATTAAT               AT^TAAT
AsnI         3882  ATTAAT               AT^TAAT
Asp700I      3666  GAAAGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        4878  GACACTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII          35  TGCGCA               TGC^GCA
BamHI         737  GGATCC               G^GATCC
BciVI        1427  GGATAC               GTATCC(6/5)
Bst1107I     1174  GTATAC               GTA^TAC
BstBI         367  TTCGAA               TT^CGAA
BstUI         944  CGCG                 CG^CG
BstZ17I      1174  GTATAC               GTA^TAC
Eam1105I     4878  GACACTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        5056  CCTTAGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI           44  GGCCGGCC             GGCCGG^CC
FspI           35  TGCGCA               TGC^GCA
HaeII          52  GGCGCT               [GA]GCGC^[CT]
HindIII      5044  AAGCTT               A^AGCTT
HpaI         3564  GTTAAC               GTT^AAC
MvnI          944  CGCG                 CG^CG
NcoI          189  CCATGG               C^CATGG
NheI          208  GCTAGC               G^CTAGC
NspV          367  TTCGAA               TT^CGAA
PflMI        2184  CCATTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1305  AGGTCCC              [GA]G^G[AT]CC[CT]
PshBI        3882  ATTAAT               AT^TAAT
SacI          936  GAGCTC               GAGCT^C
SfuI          367  TTCGAA               TT^CGAA
SphI         1261  GCATGC               GCATG^C
StuI          846  AGGCCT               AGG^CCT
Van91I       2184  CCATTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         3666  GAAAGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccI         1174  GTATAC               GT^[AC][GT]AC
             5233  GTCTAC              
AflII        4464  CTTAAG               C^TTAAG
             4771  CTTAAG              
AocI          849  CCTCAGG              CC^T[ACGT]AGG
             3147  CCTTAGG             
AvaI         1404  CCCGAG               C^[CT]CG[GA]G
             5175  CCCGAG              
BalI          297  TGGCCA               TGG^CCA
             4986  TGGCCA              
BbsI         1399  GTCTTC               GAAGAC(2/6)
             1664  GTCTTC              
BfrI         4464  CTTAAG               C^TTAAG
             4771  CTTAAG              
BglI         1343  GCCAGCGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4684  GCCCTGTTGGC         
BpuAI        1399  GTCTTC               GAAGAC(2/6)
             1664  GTCTTC              
BsaAI        1177  TACGTA               [CT]AC^GT[GA]
             2103  TACGTA              
BsaBI         503  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1737  GATGCCCATC          
BsaI         3005  GAGACC               GGTCTC(1/5)
              854  GGTCTC              
BsiEI          43  CGGCCG               CG[GA][CT]^CG
               47  CGGCCG              
BsoBI        1404  CCCGAG               C^[CT]CG[GA]G
             5175  CCCGAG              
Bsp1407I     2737  TGTACA               T^GTACA
             5325  TGTACA              
BsrGI        2737  TGTACA               T^GTACA
             5325  TGTACA              
BstAPI        127  GCAACAGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2986  GCATTGCCTGC         
BstXI         229  CCACTCTCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2334  CCATTAAGGTGG        
Bsu36I        849  CCTCAGG              CC^T[ACGT]AGG
             3147  CCTTAGG             
DsaI          189  CCATGG               C^C[GA][CT]GG
             3170  CCGTGG              
EagI           43  CGGCCG               C^GGCCG
               47  CGGCCG              
EclXI          43  CGGCCG               C^GGCCG
               47  CGGCCG              
Eco52I         43  CGGCCG               C^GGCCG
               47  CGGCCG              
Eco81I        849  CCTCAGG              CC^T[ACGT]AGG
             3147  CCTTAGG             
MaeII        1178  ACGT                 A^CGT
             2104  ACGT                
MamI          503  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1737  GATGCCCATC          
MluNI         297  TGGCCA               TGG^CCA
             4986  TGGCCA              
MscI          297  TGGCCA               TGG^CCA
             4986  TGGCCA              
PvuII        4733  CAGCTG               CAG^CTG
             4938  CAGCTG              
SexAI        1302  ACCAGGT              A^CC[AT]GGT
             4034  ACCAGGT             
SnaBI        1177  TACGTA               TAC^GTA
             2103  TACGTA              
SpeI         4236  ACTAGT               A^CTAGT
             5114  ACTAGT              
SspBI        2737  TGTACA               T^GTACA
             5325  TGTACA              
XbaI          412  TCTAGA               T^CTAGA
             5487  TCTAGA              
XcmI         1384  CCAATCCTACATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3858  CCATAAAGGATTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApaI        ApoI        AspHI       
AvaII       BanI        BanII       BbvI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BspLU11I    BspMI       BsrDI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraI        DraII       EaeI        EarI        
EcoO109I    EcoRI       EcoRII      EcoT14I     EcoT22I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MfeI        MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MunI        MvaI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       NsiI        
NspI        PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII