################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI Aor51HI AscI Asp718I AspI AvrII BbeI BbrPI BbvCI BcgI BclI BglII BlnI BsaHI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspHI BsrBI BssHII BssSI BstEII BstPI ClaI CpoI DraIII DrdI Eco47III EcoO65I EcoRV FbaI HgaI Hin1I KasI KpnI KspI MluI MroI NarI NdeI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccII 944 CGCG CG^CG AhdI 4878 GACACTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1298 GTGCAC G^TGCAC ApaLI 1298 GTGCAC G^TGCAC AseI 3882 ATTAAT AT^TAAT AsnI 3882 ATTAAT AT^TAAT Asp700I 3666 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 4878 GACACTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 35 TGCGCA TGC^GCA BamHI 737 GGATCC G^GATCC BciVI 1427 GGATAC GTATCC(6/5) Bst1107I 1174 GTATAC GTA^TAC BstBI 367 TTCGAA TT^CGAA BstUI 944 CGCG CG^CG BstZ17I 1174 GTATAC GTA^TAC Eam1105I 4878 GACACTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 5056 CCTTAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FseI 44 GGCCGGCC GGCCGG^CC FspI 35 TGCGCA TGC^GCA HaeII 52 GGCGCT [GA]GCGC^[CT] HindIII 5044 AAGCTT A^AGCTT HpaI 3564 GTTAAC GTT^AAC MvnI 944 CGCG CG^CG NcoI 189 CCATGG C^CATGG NheI 208 GCTAGC G^CTAGC NspV 367 TTCGAA TT^CGAA PflMI 2184 CCATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1305 AGGTCCC [GA]G^G[AT]CC[CT] PshBI 3882 ATTAAT AT^TAAT SacI 936 GAGCTC GAGCT^C SfuI 367 TTCGAA TT^CGAA SphI 1261 GCATGC GCATG^C StuI 846 AGGCCT AGG^CCT Van91I 2184 CCATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 3666 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 1174 GTATAC GT^[AC][GT]AC 5233 GTCTAC AflII 4464 CTTAAG C^TTAAG 4771 CTTAAG AocI 849 CCTCAGG CC^T[ACGT]AGG 3147 CCTTAGG AvaI 1404 CCCGAG C^[CT]CG[GA]G 5175 CCCGAG BalI 297 TGGCCA TGG^CCA 4986 TGGCCA BbsI 1399 GTCTTC GAAGAC(2/6) 1664 GTCTTC BfrI 4464 CTTAAG C^TTAAG 4771 CTTAAG BglI 1343 GCCAGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4684 GCCCTGTTGGC BpuAI 1399 GTCTTC GAAGAC(2/6) 1664 GTCTTC BsaAI 1177 TACGTA [CT]AC^GT[GA] 2103 TACGTA BsaBI 503 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1737 GATGCCCATC BsaI 3005 GAGACC GGTCTC(1/5) 854 GGTCTC BsiEI 43 CGGCCG CG[GA][CT]^CG 47 CGGCCG BsoBI 1404 CCCGAG C^[CT]CG[GA]G 5175 CCCGAG Bsp1407I 2737 TGTACA T^GTACA 5325 TGTACA BsrGI 2737 TGTACA T^GTACA 5325 TGTACA BstAPI 127 GCAACAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2986 GCATTGCCTGC BstXI 229 CCACTCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2334 CCATTAAGGTGG Bsu36I 849 CCTCAGG CC^T[ACGT]AGG 3147 CCTTAGG DsaI 189 CCATGG C^C[GA][CT]GG 3170 CCGTGG EagI 43 CGGCCG C^GGCCG 47 CGGCCG EclXI 43 CGGCCG C^GGCCG 47 CGGCCG Eco52I 43 CGGCCG C^GGCCG 47 CGGCCG Eco81I 849 CCTCAGG CC^T[ACGT]AGG 3147 CCTTAGG MaeII 1178 ACGT A^CGT 2104 ACGT MamI 503 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1737 GATGCCCATC MluNI 297 TGGCCA TGG^CCA 4986 TGGCCA MscI 297 TGGCCA TGG^CCA 4986 TGGCCA PvuII 4733 CAGCTG CAG^CTG 4938 CAGCTG SexAI 1302 ACCAGGT A^CC[AT]GGT 4034 ACCAGGT SnaBI 1177 TACGTA TAC^GTA 2103 TACGTA SpeI 4236 ACTAGT A^CTAGT 5114 ACTAGT SspBI 2737 TGTACA T^GTACA 5325 TGTACA XbaI 412 TCTAGA T^CTAGA 5487 TCTAGA XcmI 1384 CCAATCCTACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3858 CCATAAAGGATTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaII BanI BanII BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspLU11I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRI EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NsiI NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII