################################## # NO CUT ENZYMES ( 47 enzymes) # ################################## AatII AclI AgeI AhdI Aor51HI AscI AspEI AspI BclI BsiWI BsmBI BspDI BssHII BssSI BstBI ClaI CpoI DsaI Eam1105I Eco47III FbaI FseI HpaI KspI MluI NcoI NotI NruI NspV PacI PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SapI SbfI SfiI SfuI SgrAI SpeI Sse8387I Tth111I XbaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 5894 GGTACC G^GTACC AccII 2028 CGCG CG^CG AccIII 2009 TCCGGA T^CCGGA AflII 4332 CTTAAG C^TTAAG Asp718I 5894 GGTACC G^GTACC AviII 290 TGCGCA TGC^GCA AvrII 4457 CCTAGG C^CTAGG BbeI 1706 GGCGCC GGCGC^C BbsI 3445 GAAGAC GAAGAC(2/6) BfrI 4332 CTTAAG C^TTAAG BlnI 4457 CCTAGG C^CTAGG BpuAI 3445 GAAGAC GAAGAC(2/6) BsaBI 4886 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2009 TCCGGA [AT]^CCGG[AT] BseAI 2009 TCCGGA T^CCGGA BsiEI 952 CGGCCG CG[GA][CT]^CG BsmI 2937 GCATTC GAATGC(1/-1) BspEI 2009 TCCGGA T^CCGGA BspLU11I 3700 ACATGT A^CATGT BstAPI 4474 GCATCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1233 GGTGACC G^GT[ACGT]ACC BstPI 1233 GGTGACC G^GT[ACGT]ACC BstUI 2028 CGCG CG^CG DrdI 4073 GACAAATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 952 CGGCCG C^GGCCG EclXI 952 CGGCCG C^GGCCG Eco52I 952 CGGCCG C^GGCCG EcoNI 474 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1233 GGTGACC G^GT[ACGT]ACC EcoRV 3249 GATATC GAT^ATC FspI 290 TGCGCA TGC^GCA KasI 1706 GGCGCC G^GCGCC KpnI 5894 GGTACC GGTAC^C MamI 4886 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2009 TCCGGA T^CCGGA MvnI 2028 CGCG CG^CG NarI 1706 GGCGCC GG^CGCC NdeI 5710 CATATG CA^TATG SacI 199 GAGCTC GAGCT^C SalI 643 GTCGAC G^TCGAC ScaI 3878 AGTACT AGT^ACT SfoI 1706 GGCGCC GGC^GCC SmaI 1142 CCCGGG CCC^GGG SnaBI 4554 TACGTA TAC^GTA StuI 596 AGGCCT AGG^CCT XmaCI 1142 CCCGGG C^CCGGG XmaI 1142 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AflIII 2382 ACGTGT A^C[GA][CT]GT 3700 ACATGT Alw44I 1857 GTGCAC G^TGCAC 2866 GTGCAC ApaI 1049 GGGCCC GGGCC^C 5628 GGGCCC ApaLI 1857 GTGCAC G^TGCAC 2866 GTGCAC AseI 1719 ATTAAT AT^TAAT 3662 ATTAAT AsnI 1719 ATTAAT AT^TAAT 3662 ATTAAT Asp700I 1275 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4435 GAAAAAGTTC BamHI 2006 GGATCC G^GATCC 4891 GGATCC BlpI 3034 GCTGAGC GC^T[ACGT]AGC 3483 GCTGAGC Bpu1102I 3034 GCTGAGC GC^T[ACGT]AGC 3483 GCTGAGC Bsp1407I 752 TGTACA T^GTACA 1493 TGTACA BspHI 3382 TCATGA T^CATGA 3845 TCATGA BsrBI 1367 GAGCGG CCGCTC(-3/-3) 2545 GAGCGG BsrGI 752 TGTACA T^GTACA 1493 TGTACA Bst1107I 4908 GTATAC GTA^TAC 6080 GTATAC BstXI 62 CCAGCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1586 CCATATTCATGG BstZ17I 4908 GTATAC GTA^TAC 6080 GTATAC CelII 3034 GCTGAGC GC^T[ACGT]AGC 3483 GCTGAGC DraIII 487 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG 5387 CACTATGTG EcoRI 766 GAATTC G^AATTC 3101 GAATTC EcoT22I 4545 ATGCAT ATGCA^T 6118 ATGCAT HindIII 2375 AAGCTT A^AGCTT 3224 AAGCTT MfeI 2811 CAATTG C^AATTG 3684 CAATTG MunI 2811 CAATTG C^AATTG 3684 CAATTG NaeI 98 GCCGGC GCC^GGC 954 GCCGGC NgoMIV 98 GCCGGC G^CCGGC 954 GCCGGC NheI 1311 GCTAGC G^CTAGC 2508 GCTAGC NsiI 4545 ATGCAT ATGCA^T 6118 ATGCAT PaeR7I 196 CTCGAG C^TCGAG 2068 CTCGAG PshBI 1719 ATTAAT AT^TAAT 3662 ATTAAT RcaI 3382 TCATGA T^CATGA 3845 TCATGA SspBI 752 TGTACA T^GTACA 1493 TGTACA SwaI 5029 ATTTAAAT ATTT^AAAT 5343 ATTTAAAT XhoI 196 CTCGAG C^TCGAG 2068 CTCGAG XmnI 1275 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4435 GAAAAAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BanI BanII BbrPI BbvCI BbvI BcgI BciVI BcnI BfaI BglI BglII BmyI BpmI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SphI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII