##################################
# NO CUT ENZYMES (   47 enzymes) #
##################################

AatII       AclI        AgeI        AhdI        Aor51HI     
AscI        AspEI       AspI        BclI        BsiWI       
BsmBI       BspDI       BssHII      BssSI       BstBI       
ClaI        CpoI        DsaI        Eam1105I    Eco47III    
FbaI        FseI        HpaI        KspI        MluI        
NcoI        NotI        NruI        NspV        PacI        
PflFI       PinAI       PmeI        PshAI       Psp1406I    
PvuI        RsrII       SacII       SapI        SbfI        
SfiI        SfuI        SgrAI       SpeI        Sse8387I    
Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I       5894  GGTACC               G^GTACC
AccII        2028  CGCG                 CG^CG
AccIII       2009  TCCGGA               T^CCGGA
AflII        4332  CTTAAG               C^TTAAG
Asp718I      5894  GGTACC               G^GTACC
AviII         290  TGCGCA               TGC^GCA
AvrII        4457  CCTAGG               C^CTAGG
BbeI         1706  GGCGCC               GGCGC^C
BbsI         3445  GAAGAC               GAAGAC(2/6)
BfrI         4332  CTTAAG               C^TTAAG
BlnI         4457  CCTAGG               C^CTAGG
BpuAI        3445  GAAGAC               GAAGAC(2/6)
BsaBI        4886  GATCGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2009  TCCGGA               [AT]^CCGG[AT]
BseAI        2009  TCCGGA               T^CCGGA
BsiEI         952  CGGCCG               CG[GA][CT]^CG
BsmI         2937  GCATTC               GAATGC(1/-1)
BspEI        2009  TCCGGA               T^CCGGA
BspLU11I     3700  ACATGT               A^CATGT
BstAPI       4474  GCATCTTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1233  GGTGACC              G^GT[ACGT]ACC
BstPI        1233  GGTGACC              G^GT[ACGT]ACC
BstUI        2028  CGCG                 CG^CG
DrdI         4073  GACAAATCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          952  CGGCCG               C^GGCCG
EclXI         952  CGGCCG               C^GGCCG
Eco52I        952  CGGCCG               C^GGCCG
EcoNI         474  CCTGAAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1233  GGTGACC              G^GT[ACGT]ACC
EcoRV        3249  GATATC               GAT^ATC
FspI          290  TGCGCA               TGC^GCA
KasI         1706  GGCGCC               G^GCGCC
KpnI         5894  GGTACC               GGTAC^C
MamI         4886  GATCGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2009  TCCGGA               T^CCGGA
MvnI         2028  CGCG                 CG^CG
NarI         1706  GGCGCC               GG^CGCC
NdeI         5710  CATATG               CA^TATG
SacI          199  GAGCTC               GAGCT^C
SalI          643  GTCGAC               G^TCGAC
ScaI         3878  AGTACT               AGT^ACT
SfoI         1706  GGCGCC               GGC^GCC
SmaI         1142  CCCGGG               CCC^GGG
SnaBI        4554  TACGTA               TAC^GTA
StuI          596  AGGCCT               AGG^CCT
XmaCI        1142  CCCGGG               C^CCGGG
XmaI         1142  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AflIII       2382  ACGTGT               A^C[GA][CT]GT
             3700  ACATGT              
Alw44I       1857  GTGCAC               G^TGCAC
             2866  GTGCAC              
ApaI         1049  GGGCCC               GGGCC^C
             5628  GGGCCC              
ApaLI        1857  GTGCAC               G^TGCAC
             2866  GTGCAC              
AseI         1719  ATTAAT               AT^TAAT
             3662  ATTAAT              
AsnI         1719  ATTAAT               AT^TAAT
             3662  ATTAAT              
Asp700I      1275  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4435  GAAAAAGTTC          
BamHI        2006  GGATCC               G^GATCC
             4891  GGATCC              
BlpI         3034  GCTGAGC              GC^T[ACGT]AGC
             3483  GCTGAGC             
Bpu1102I     3034  GCTGAGC              GC^T[ACGT]AGC
             3483  GCTGAGC             
Bsp1407I      752  TGTACA               T^GTACA
             1493  TGTACA              
BspHI        3382  TCATGA               T^CATGA
             3845  TCATGA              
BsrBI        1367  GAGCGG               CCGCTC(-3/-3)
             2545  GAGCGG              
BsrGI         752  TGTACA               T^GTACA
             1493  TGTACA              
Bst1107I     4908  GTATAC               GTA^TAC
             6080  GTATAC              
BstXI          62  CCAGCATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1586  CCATATTCATGG        
BstZ17I      4908  GTATAC               GTA^TAC
             6080  GTATAC              
CelII        3034  GCTGAGC              GC^T[ACGT]AGC
             3483  GCTGAGC             
DraIII        487  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             5387  CACTATGTG           
EcoRI         766  GAATTC               G^AATTC
             3101  GAATTC              
EcoT22I      4545  ATGCAT               ATGCA^T
             6118  ATGCAT              
HindIII      2375  AAGCTT               A^AGCTT
             3224  AAGCTT              
MfeI         2811  CAATTG               C^AATTG
             3684  CAATTG              
MunI         2811  CAATTG               C^AATTG
             3684  CAATTG              
NaeI           98  GCCGGC               GCC^GGC
              954  GCCGGC              
NgoMIV         98  GCCGGC               G^CCGGC
              954  GCCGGC              
NheI         1311  GCTAGC               G^CTAGC
             2508  GCTAGC              
NsiI         4545  ATGCAT               ATGCA^T
             6118  ATGCAT              
PaeR7I        196  CTCGAG               C^TCGAG
             2068  CTCGAG              
PshBI        1719  ATTAAT               AT^TAAT
             3662  ATTAAT              
RcaI         3382  TCATGA               T^CATGA
             3845  TCATGA              
SspBI         752  TGTACA               T^GTACA
             1493  TGTACA              
SwaI         5029  ATTTAAAT             ATTT^AAAT
             5343  ATTTAAAT            
XhoI          196  CTCGAG               C^TCGAG
             2068  CTCGAG              
XmnI         1275  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4435  GAAAAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  129 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApoI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbrPI       BbvCI       BbvI        BcgI        
BciVI       BcnI        BfaI        BglI        BglII       
BmyI        BpmI        BsaAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrDI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraI        DraII       EaeI        EarI        Eco81I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PmaCI       PmlI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SmlI        
SphI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII