##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccIII AgeI AhdI Aor51HI
ApaI AscI AspEI AspI AviII
AvrII BalI BbeI BbrPI BbvCI
BcgI BlnI BlpI Bpu1102I BsaWI
BseAI BsiWI BsmBI BspEI BsrBI
BsrFI BssHII BstAPI CelII Cfr10I
CpoI DraII DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
FseI FspI HaeII HpaI KasI
KspI MluI MluNI MroI MscI
NaeI NarI NgoMIV NheI NotI
NruI PaeR7I PflFI PinAI PmaCI
PmlI PpuMI PshAI PvuI RsrII
SacII SalI ScaI SexAI SfiI
SfoI SgrAI SmaI SpeI SphI
StuI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 4058 GTATAC GT^[AC][GT]AC
AclI 3081 AACGTT AA^CGTT
AflII 1893 CTTAAG C^TTAAG
AvaI 3714 CTCGGG C^[CT]CG[GA]G
BamHI 3134 GGATCC G^GATCC
BciVI 2108 GTATCC GTATCC(6/5)
BclI 5184 TGATCA T^GATCA
BfrI 1893 CTTAAG C^TTAAG
BglI 4511 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 208 CGACCG CG[GA][CT]^CG
BsoBI 3714 CTCGGG C^[CT]CG[GA]G
Bsp1407I 2993 TGTACA T^GTACA
BspDI 4451 ATCGAT AT^CGAT
BspLU11I 5682 ACATGT A^CATGT
BsrGI 2993 TGTACA T^GTACA
BssSI 1372 CACGAG CACGAG(-5/-1)
Bst1107I 4058 GTATAC GTA^TAC
BstBI 2762 TTCGAA TT^CGAA
BstEII 159 GGTAACC G^GT[ACGT]ACC
BstPI 159 GGTAACC G^GT[ACGT]ACC
BstZ17I 4058 GTATAC GTA^TAC
ClaI 4451 ATCGAT AT^CGAT
DrdI 401 GACCTGTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3669 CCTACTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 159 GGTAACC G^GT[ACGT]ACC
EcoRI 5107 GAATTC G^AATTC
FbaI 5184 TGATCA T^GATCA
NdeI 4839 CATATG CA^TATG
NspI 5682 ACATGT [GA]CATG^[CT]
NspV 2762 TTCGAA TT^CGAA
PflMI 3852 CCAACCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 3350 GTTTAAAC GTTT^AAAC
Psp1406I 3081 AACGTT AA^CGTT
SacI 3093 GAGCTC GAGCT^C
SapI 2089 GAAGAGC GCTCTTC(1/4)
SbfI 2058 CCTGCAGG CCTGCA^GG
SfuI 2762 TTCGAA TT^CGAA
Sse8387I 2058 CCTGCAGG CCTGCA^GG
SspBI 2993 TGTACA T^GTACA
SwaI 716 ATTTAAAT ATTT^AAAT
Van91I 3852 CCAACCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 768 CCAGTGGAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 3471 GGTACC G^GTACC
5601 GGTACC
AccII 201 CGCG CG^CG
3807 CGCG
AcyI 3508 GGCGTC G[GA]^CG[CT]C
3798 GACGCC
AflIII 2360 ACGTGT A^C[GA][CT]GT
5682 ACATGT
AlwNI 4250 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
4557 CAGAGTCTG
Asp718I 3471 GGTACC G^GTACC
5601 GGTACC
BanII 3093 GAGCTC G[GA]GC[CT]^C
3437 GAGCCC
BcnI 145 CCGGG CC^[GC]GG
3192 CCGGG
BglII 3405 AGATCT A^GATCT
5054 AGATCT
BsaAI 2611 TACGTA [CT]AC^GT[GA]
4055 TACGTA
BsaHI 3508 GGCGTC G[GA]^CG[CT]C
3798 GACGCC
BsaI 3275 GAGACC GGTCTC(1/5)
3679 GGTCTC
BsgI 1078 GTGCAG GTGCAG(16/14)
4147 GTGCAG
BsmFI 4662 GGGAC GGGAC(10/14)
5389 GGGAC
BspHI 2378 TCATGA T^CATGA
4381 TCATGA
BstUI 201 CGCG CG^CG
3807 CGCG
CfoI 3730 GCGC GCG^C
5273 GCGC
FauI 5 GCGGG CCCGC(4/6)
3690 GCGGG
HapII 145 CCGG C^CGG
3192 CCGG
HhaI 3730 GCGC GCG^C
5273 GCGC
Hin1I 3508 GGCGTC G[GA]^CG[CT]C
3798 GACGCC
HinP1I 3730 GCGC G^CGC
5273 GCGC
HincII 1949 GTTGAC GT[CT]^[GA]AC
5009 GTTGAC
HindII 1949 GTTGAC GT[CT]^[GA]AC
5009 GTTGAC
HpaII 145 CCGG C^CGG
3192 CCGG
KpnI 3471 GGTACC GGTAC^C
5601 GGTACC
MfeI 1612 CAATTG C^AATTG
4724 CAATTG
MspI 145 CCGG C^CGG
3192 CCGG
MunI 1612 CAATTG C^AATTG
4724 CAATTG
MvnI 201 CGCG CG^CG
3807 CGCG
NciI 145 CCGGG CC^[GC]GG
3192 CCGGG
PacI 2472 TTAATTAA TTAAT^TAA
5552 TTAATTAA
PvuII 2230 CAGCTG CAG^CTG
4845 CAGCTG
RcaI 2378 TCATGA T^CATGA
4381 TCATGA
SfaNI 4606 GCATC GCATC(5/9)
5139 GCATC
SmlI 247 CTCAAG C^T[CT][GA]AG
1893 CTTAAG
SnaBI 2611 TACGTA TAC^GTA
4055 TACGTA
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AocI ApaLI ApoI AseI
AsnI Asp700I AspHI AvaII BanI
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaBI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI DsaI
EarI Eco81I EcoRII EcoRV EcoT14I
EcoT22I Fnu4HI FokI HaeIII HgaI
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NcoI NdeII
NlaIII NlaIV NsiI PleI PshBI
PstI RsaI Sau3AI Sau96I ScrFI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII XmnI