##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AclI AflII AscI AseI AsnI
AspI AvrII BciVI BclI BfrI
BlnI BsaBI BsiWI BsmI BsrDI
BssHII BstBI CpoI EcoRV EcoT22I
FbaI FseI HindIII HpaI MamI
MfeI MluI MunI NotI NruI
NsiI NspV PacI PflFI PmeI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SfiI SfuI SnaBI
SpeI Sse8387I SspI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 3612 GACGTC GACGT^C
Acc65I 73 GGTACC G^GTACC
AccI 5020 GTATAC GT^[AC][GT]AC
AflIII 4060 ACATGT A^C[GA][CT]GT
AgeI 70 ACCGGT A^CCGGT
AhdI 4146 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1122 GTGCAC G^TGCAC
ApaLI 1122 GTGCAC G^TGCAC
Asp700I 4058 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 73 GGTACC G^GTACC
AspEI 4146 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2085 GGCGCC GGCGC^C
BcgI 3110 CGAGGCGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2282 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2282 GCTGAGC GC^T[ACGT]AGC
BsiEI 349 CGGCCG CG[GA][CT]^CG
Bsp1407I 4551 TGTACA T^GTACA
BspDI 3135 ATCGAT AT^CGAT
BspLU11I 4060 ACATGT A^CATGT
BsrGI 4551 TGTACA T^GTACA
Bst1107I 5020 GTATAC GTA^TAC
BstEII 3332 GGTAACC G^GT[ACGT]ACC
BstPI 3332 GGTAACC G^GT[ACGT]ACC
BstZ17I 5020 GTATAC GTA^TAC
CelII 2282 GCTGAGC GC^T[ACGT]AGC
ClaI 3135 ATCGAT AT^CGAT
DraI 4933 TTTAAA TTT^AAA
DraIII 229 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4218 GACCGCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 349 CGGCCG C^GGCCG
Eam1105I 4146 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 349 CGGCCG C^GGCCG
Eco52I 349 CGGCCG C^GGCCG
EcoO65I 3332 GGTAACC G^GT[ACGT]ACC
EcoRI 3654 GAATTC G^AATTC
HincII 4227 GTCAAC GT[CT]^[GA]AC
HindII 4227 GTCAAC GT[CT]^[GA]AC
KasI 2085 GGCGCC G^GCGCC
KpnI 73 GGTACC GGTAC^C
KspI 1220 CCGCGG CCGC^GG
NarI 2085 GGCGCC GG^CGCC
NdeI 4432 CATATG CA^TATG
NheI 2448 GCTAGC G^CTAGC
PinAI 70 ACCGGT A^CCGGT
PshAI 3844 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1220 CCGCGG CCGC^GG
SexAI 5131 ACCTGGT A^CC[AT]GGT
SfoI 2085 GGCGCC GGC^GCC
SmaI 2089 CCCGGG CCC^GGG
SphI 3386 GCATGC GCATG^C
SspBI 4551 TGTACA T^GTACA
SwaI 4932 ATTTAAAT ATTT^AAAT
XmaCI 2089 CCCGGG C^CCGGG
XmaI 2089 CCCGGG C^CCGGG
XmnI 4058 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 2638 TCCGGA T^CCGGA
3460 TCCGGA
AcsI 3654 GAATTC [GA]^AATT[CT]
5106 AAATTT
Aor51HI 324 AGCGCT AGC^GCT
3296 AGCGCT
ApaI 2023 GGGCCC GGGCC^C
4698 GGGCCC
ApoI 3654 GAATTC [GA]^AATT[CT]
5106 AAATTT
AviII 19 TGCGCA TGC^GCA
2005 TGCGCA
BamHI 592 GGATCC G^GATCC
1838 GGATCC
BbrPI 1233 CACGTG CAC^GTG
1473 CACGTG
BseAI 2638 TCCGGA T^CCGGA
3460 TCCGGA
BsmBI 2938 CGTCTC CGTCTC(1/5)
1969 GAGACG
BspEI 2638 TCCGGA T^CCGGA
3460 TCCGGA
BspHI 4575 TCATGA T^CATGA
4794 TCATGA
BssSI 903 CACGAG CACGAG(-5/-1)
3790 CACGAG
Eco47III 324 AGCGCT AGC^GCT
3296 AGCGCT
FspI 19 TGCGCA TGC^GCA
2005 TGCGCA
MroI 2638 TCCGGA T^CCGGA
3460 TCCGGA
NaeI 3193 GCCGGC GCC^GGC
3625 GCCGGC
NcoI 982 CCATGG C^CATGG
2329 CCATGG
NgoMIV 3193 GCCGGC G^CCGGC
3625 GCCGGC
NspI 3386 GCATGC [GA]CATG^[CT]
4060 ACATGT
PmaCI 1233 CACGTG CAC^GTG
1473 CACGTG
PmlI 1233 CACGTG CAC^GTG
1473 CACGTG
RcaI 4575 TCATGA T^CATGA
4794 TCATGA
SgrAI 3192 CGCCGGCG C[GA]^CCGG[CT]G
3423 CACCGGCG
StuI 3098 AGGCCT AGG^CCT
4692 AGGCCT
XcmI 2923 CCAAGGAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4243 CCATCCAGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BglII BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PaeR7I PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoI XhoII