##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AhdI AocI Aor51HI AscI Asp718I
AspEI AvrII BbrPI BciVI BfrI
BglII BlnI BsaAI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstXI BstZ17I Bsu36I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I FseI KpnI
MfeI MunI NdeI NotI NruI
PacI PaeR7I PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SalI
ScaI SfiI SgrAI SnaBI SpeI
SspBI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 5548 TCCGGA T^CCGGA
AgeI 2482 ACCGGT A^CCGGT
ApaI 4714 GGGCCC GGGCC^C
AviII 2392 TGCGCA TGC^GCA
BamHI 614 GGATCC G^GATCC
BclI 1115 TGATCA T^GATCA
BglI 4949 GCCCCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 609 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 5548 TCCGGA T^CCGGA
BsiEI 3497 CGACCG CG[GA][CT]^CG
BsmBI 3694 CGTCTC CGTCTC(1/5)
BspEI 5548 TCCGGA T^CCGGA
BstBI 1650 TTCGAA TT^CGAA
BstEII 1790 GGTGACC G^GT[ACGT]ACC
BstPI 1790 GGTGACC G^GT[ACGT]ACC
EcoO65I 1790 GGTGACC G^GT[ACGT]ACC
EcoRV 1875 GATATC GAT^ATC
EcoT22I 1120 ATGCAT ATGCA^T
FbaI 1115 TGATCA T^GATCA
FspI 2392 TGCGCA TGC^GCA
HindIII 1092 AAGCTT A^AGCTT
KspI 2662 CCGCGG CCGC^GG
MamI 609 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 5548 TCCGGA T^CCGGA
NaeI 4609 GCCGGC GCC^GGC
NgoMIV 4609 GCCGGC G^CCGGC
NsiI 1120 ATGCAT ATGCA^T
NspV 1650 TTCGAA TT^CGAA
PinAI 2482 ACCGGT A^CCGGT
PshAI 388 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 2662 CCGCGG CCGC^GG
SapI 4841 GAAGAGC GCTCTTC(1/4)
SbfI 4136 CCTGCAGG CCTGCA^GG
SfuI 1650 TTCGAA TT^CGAA
SphI 2395 GCATGC GCATG^C
Sse8387I 4136 CCTGCAGG CCTGCA^GG
SspI 398 AATATT AAT^ATT
StuI 239 AGGCCT AGG^CCT
SwaI 5745 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Alw44I 229 GTGCAC G^TGCAC
1928 GTGCAC
ApaLI 229 GTGCAC G^TGCAC
1928 GTGCAC
AseI 4299 ATTAAT AT^TAAT
5720 ATTAAT
AsnI 4299 ATTAAT AT^TAAT
5720 ATTAAT
Asp700I 522 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5654 GAAGAGTTTC
BsaWI 2482 ACCGGT [AT]^CCGG[AT]
5548 TCCGGA
EcoRI 105 GAATTC G^AATTC
3814 GAATTC
HincII 3041 GTTAAC GT[CT]^[GA]AC
3951 GTTAAC
HindII 3041 GTTAAC GT[CT]^[GA]AC
3951 GTTAAC
HpaI 3041 GTTAAC GTT^AAC
3951 GTTAAC
MluI 1857 ACGCGT A^CGCGT
3212 ACGCGT
NheI 471 GCTAGC G^CTAGC
1323 GCTAGC
PshBI 4299 ATTAAT AT^TAAT
5720 ATTAAT
SmaI 625 CCCGGG CCC^GGG
673 CCCGGG
XmaCI 625 CCCGGG C^CCGGG
673 CCCGGG
XmaI 625 CCCGGG C^CCGGG
673 CCCGGG
XmnI 522 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5654 GAAGAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcgI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DrdI DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfoI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII