##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AspEI AspI AviII AvrII BbrPI
BcgI BclI BglI BlnI BlpI
Bpu1102I BseAI BsiWI BsmBI BspEI
BsrFI BssHII BstBI BstEII BstPI
BstXI Bsu36I CelII Cfr10I CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FbaI FseI FspI HpaI
KspI MluI MroI NaeI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacI SacII SbfI
SfiI SfuI SgrAI SnaBI Sse8387I
SwaI Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 961 GGTACC G^GTACC
AccII 92 CGCG CG^CG
AcyI 102 GGCGCC G[GA]^CG[CT]C
AflII 2526 CTTAAG C^TTAAG
AflIII 3901 ACATGT A^C[GA][CT]GT
Asp718I 961 GGTACC G^GTACC
AvaI 1035 CCCGGG C^[CT]CG[GA]G
BamHI 174 GGATCC G^GATCC
BbeI 102 GGCGCC GGCGC^C
BbvCI 3594 CCTCAGC CCTCAGC(-5/-2)
BfrI 2526 CTTAAG C^TTAAG
BglII 1654 AGATCT A^GATCT
BsaAI 4660 TACGTG [CT]AC^GT[GA]
BsaHI 102 GGCGCC G[GA]^CG[CT]C
BsaI 1404 GAGACC GGTCTC(1/5)
BsaWI 2006 ACCGGA [AT]^CCGG[AT]
BsgI 588 CTGCAC GTGCAG(16/14)
BsiEI 295 CGACCG CG[GA][CT]^CG
BsmI 3499 GCATTC GAATGC(1/-1)
BsoBI 1035 CCCGGG C^[CT]CG[GA]G
BspHI 5581 TCATGA T^CATGA
BspLU11I 3901 ACATGT A^CATGT
BssSI 167 CACGAG CACGAG(-5/-1)
Bst1107I 3243 GTATAC GTA^TAC
BstAPI 3871 GCACCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 92 CGCG CG^CG
BstZ17I 3243 GTATAC GTA^TAC
DraII 968 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoNI 3812 CCTTTAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 968 GGGGCCC [GA]G^G[ACGT]CC[CT]
HaeII 102 GGCGCC [GA]GCGC^[CT]
HgaI 90 GACGC GACGC(5/10)
Hin1I 102 GGCGCC G[GA]^CG[CT]C
HincII 474 GTCGAC GT[CT]^[GA]AC
HindII 474 GTCGAC GT[CT]^[GA]AC
KasI 102 GGCGCC G^GCGCC
KpnI 961 GGTACC GGTAC^C
MfeI 3042 CAATTG C^AATTG
MunI 3042 CAATTG C^AATTG
MvnI 92 CGCG CG^CG
NarI 102 GGCGCC GG^CGCC
NdeI 3149 CATATG CA^TATG
RcaI 5581 TCATGA T^CATGA
SalI 474 GTCGAC G^TCGAC
SexAI 2037 ACCTGGT A^CC[AT]GGT
SfoI 102 GGCGCC GGC^GCC
SmaI 1035 CCCGGG CCC^GGG
SphI 2217 GCATGC GCATG^C
XmaCI 1035 CCCGGG C^CCGGG
XmaI 1035 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
ApaI 215 GGGCCC GGGCC^C
969 GGGCCC
AvaII 559 GGACC G^G[AT]CC
2631 GGTCC
BalI 3286 TGGCCA TGG^CCA
4343 TGGCCA
BanII 215 GGGCCC G[GA]GC[CT]^C
969 GGGCCC
BcnI 1035 CCCGG CC^[GC]GG
1036 CCGGG
BsaBI 1655 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1789 GATTATTATC
BseRI 372 GAGGAG GAGGAG(10/8)
5641 GAGGAG
Bsp1407I 543 TGTACA T^GTACA
1506 TGTACA
BspDI 645 ATCGAT AT^CGAT
1002 ATCGAT
BsrGI 543 TGTACA T^GTACA
1506 TGTACA
BstYI 174 GGATCC [GA]^GATC[CT]
1654 AGATCT
ClaI 645 ATCGAT AT^CGAT
1002 ATCGAT
EaeI 3286 TGGCCA [CT]^GGCC[GA]
4343 TGGCCA
EcoT14I 2375 CCTTGG C^C[AT][AT]GG
3195 CCTTGG
HapII 1036 CCGG C^CGG
2007 CCGG
HpaII 1036 CCGG C^CGG
2007 CCGG
MamI 1655 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1789 GATTATTATC
MflI 174 GGATCC [GA]^GATC[CT]
1654 AGATCT
MluNI 3286 TGGCCA TGG^CCA
4343 TGGCCA
MscI 3286 TGGCCA TGG^CCA
4343 TGGCCA
MspI 1036 CCGG C^CGG
2007 CCGG
NciI 1035 CCCGG CC^[GC]GG
1036 CCGGG
PvuII 1929 CAGCTG CAG^CTG
1982 CAGCTG
SapI 458 GAAGAGC GCTCTTC(1/4)
2943 GCTCTTC
ScaI 893 AGTACT AGT^ACT
1827 AGTACT
SspBI 543 TGTACA T^GTACA
1506 TGTACA
StuI 3192 AGGCCT AGG^CCT
3348 AGGCCT
StyI 2375 CCTTGG C^C[AT][AT]GG
3195 CCTTGG
Tsp45I 401 GTGAC ^GT[GC]AC
5094 GTGAC
XbaI 610 TCTAGA T^CTAGA
4225 TCTAGA
XhoII 174 GGATCC [GA]^GATC[CT]
1654 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI BanI BbsI BbvI
BciVI BfaI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrDI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EarI EcoRI EcoRII EcoT22I FauI
Fnu4HI FokI HaeIII HhaI HinP1I
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SpeI SspI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I Van91I XmnI