##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AvrII BbeI
BbrPI BcgI BfrI BlnI BsaBI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BssHII BstAPI BstBI BstEII
BstPI ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoT22I FseI HindIII HpaI KasI
KpnI KspI MamI MfeI MroI
MunI NarI NdeI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SwaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 2500 GGCGTC G[GA]^CG[CT]C
AhdI 2311 GACGAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2764 CCTCAGG CC^T[ACGT]AGG
AspEI 2311 GACGAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 30 TGCGCA TGC^GCA
BciVI 2334 GGATAC GTATCC(6/5)
BglI 3904 GCCTACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2263 AGATCT A^GATCT
BsaAI 4353 CACGTA [CT]AC^GT[GA]
BsaHI 2500 GGCGTC G[GA]^CG[CT]C
BsmBI 2502 CGTCTC CGTCTC(1/5)
BspLU11I 3786 ACATGT A^CATGT
BsrDI 1593 CATTGC GCAATG(2/0)
BsrFI 1208 GCCGGC [GA]^CCGG[CT]
BssSI 1168 CACGAG CACGAG(-5/-1)
Bst1107I 5095 GTATAC GTA^TAC
BstZ17I 5095 GTATAC GTA^TAC
Bsu36I 2764 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1208 GCCGGC [GA]^CCGG[CT]
DraI 5133 TTTAAA TTT^AAA
Eam1105I 2311 GACGAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2764 CCTCAGG CC^T[ACGT]AGG
EcoRI 1887 GAATTC G^AATTC
FspI 30 TGCGCA TGC^GCA
Hin1I 2500 GGCGTC G[GA]^CG[CT]C
MluI 4369 ACGCGT A^CGCGT
NaeI 1208 GCCGGC GCC^GGC
NgoMIV 1208 GCCGGC G^CCGGC
NheI 1204 GCTAGC G^CTAGC
PaeR7I 3409 CTCGAG C^TCGAG
PshAI 2299 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 1323 CCTGCAGG CCTGCA^GG
ScaI 2933 AGTACT AGT^ACT
Sse8387I 1323 CCTGCAGG CCTGCA^GG
XbaI 1050 TCTAGA T^CTAGA
XhoI 3409 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 3625 GTAGAC GT^[AC][GT]AC
5095 GTATAC
Asp700I 561 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5189 GAAAATTTTC
AspHI 1231 GAGCTC G[AT]GC[AT]^C
1555 GAGCTC
AspI 1828 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3036 GACTTTGTC
BamHI 61 GGATCC G^GATCC
3769 GGATCC
BsiHKAI 1231 GAGCTC G[AT]GC[AT]^C
1555 GAGCTC
Bsp1407I 4776 TGTACA T^GTACA
4870 TGTACA
BsrGI 4776 TGTACA T^GTACA
4870 TGTACA
BstXI 836 CCACCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2339 CCACAGTCCTGG
EcoNI 182 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4445 CCTTGGAGAGG
EcoRV 492 GATATC GAT^ATC
799 GATATC
HaeII 2200 AGCGCC [GA]GCGC^[CT]
2832 GGCGCT
HgaI 2501 GCGTC GACGC(5/10)
3524 GCGTC
HincII 1969 GTCAAC GT[CT]^[GA]AC
4983 GTTGAC
HindII 1969 GTCAAC GT[CT]^[GA]AC
4983 GTTGAC
PflFI 1828 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3036 GACTTTGTC
SacI 1231 GAGCTC GAGCT^C
1555 GAGCTC
SspBI 4776 TGTACA T^GTACA
4870 TGTACA
SspI 4239 AATATT AAT^ATT
4975 AATATT
StuI 1513 AGGCCT AGG^CCT
1954 AGGCCT
Tth111I 1828 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3036 GACTTTGTC
XmnI 561 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5189 GAAAATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BclI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI