##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccIII AclI AocI Aor51HI
ApaI AscI Asp718I AspI AvrII
BamHI BanII BbeI BciVI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BseAI BsiEI BsiWI BspDI BspEI
BspLU11I BsrBI BssHII BssSI BstBI
BstEII BstPI BstYI Bsu36I CelII
ClaI CpoI DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FauI FseI
HaeII HpaI KasI KpnI KspI
MamI MflI MroI MspA1I NaeI
NarI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PmeI PshAI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I Tth111I
XhoI XhoII
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 858 GACGTC GACGT^C
AccII 1853 CGCG CG^CG
AciI 210 CCGC CCGC(-3/-1)
AcyI 858 GACGTC G[GA]^CG[CT]C
AflII 2421 CTTAAG C^TTAAG
AgeI 3667 ACCGGT A^CCGGT
AhdI 1480 GACACATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4014 GTGCAC G^TGCAC
ApaLI 4014 GTGCAC G^TGCAC
AseI 3246 ATTAAT AT^TAAT
AsnI 3246 ATTAAT AT^TAAT
AspEI 1480 GACACATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3078 TGCGCA TGC^GCA
BalI 4635 TGGCCA TGG^CCA
BbrPI 2066 CACGTG CAC^GTG
BbvCI 4588 CCTCAGC CCTCAGC(-5/-2)
BcgI 497 CGATTCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 704 TGATCA T^GATCA
BfrI 2421 CTTAAG C^TTAAG
BsaAI 2066 CACGTG [CT]AC^GT[GA]
BsaHI 858 GACGTC G[GA]^CG[CT]C
BsaI 2053 GAGACC GGTCTC(1/5)
BsaWI 3667 ACCGGT [AT]^CCGG[AT]
BsmBI 2111 CGTCTC CGTCTC(1/5)
BsrFI 3667 ACCGGT [GA]^CCGG[CT]
BstUI 1853 CGCG CG^CG
CfoI 3079 GCGC GCG^C
Cfr10I 3667 ACCGGT [GA]^CCGG[CT]
DraIII 4711 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 4635 TGGCCA [CT]^GGCC[GA]
Eam1105I 1480 GACACATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 704 TGATCA T^GATCA
FspI 3078 TGCGCA TGC^GCA
HhaI 3079 GCGC GCG^C
Hin1I 858 GACGTC G[GA]^CG[CT]C
HinP1I 3079 GCGC G^CGC
MfeI 1403 CAATTG C^AATTG
MluI 1852 ACGCGT A^CGCGT
MluNI 4635 TGGCCA TGG^CCA
MscI 4635 TGGCCA TGG^CCA
MunI 1403 CAATTG C^AATTG
MvnI 1853 CGCG CG^CG
PinAI 3667 ACCGGT A^CCGGT
PmaCI 2066 CACGTG CAC^GTG
PmlI 2066 CACGTG CAC^GTG
PshBI 3246 ATTAAT AT^TAAT
PstI 214 CTGCAG CTGCA^G
SexAI 716 ACCTGGT A^CC[AT]GGT
SspI 2903 AATATT AAT^ATT
StuI 52 AGGCCT AGG^CCT
SwaI 2582 ATTTAAAT ATTT^AAAT
XbaI 3477 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 2860 GTATAC GT^[AC][GT]AC
3760 GTATAC
AlwI 600 GATCC GGATC(4/5)
2062 GGATC
AvaI 70 CCCGGG C^[CT]CG[GA]G
730 CCCGGG
AvaII 3582 GGTCC G^G[AT]CC
4695 GGTCC
BanI 141 GGCACC G^G[CT][GA]CC
4471 GGCACC
BseRI 177 GAGGAG GAGGAG(10/8)
1914 GAGGAG
BsgI 402 GTGCAG GTGCAG(16/14)
2948 GTGCAG
BsmI 1325 GAATGC GAATGC(1/-1)
632 GCATTC
BsoBI 70 CCCGGG C^[CT]CG[GA]G
730 CCCGGG
Bsp1407I 2975 TGTACA T^GTACA
4739 TGTACA
BspMI 3075 ACCTGC ACCTGC(4/8)
1035 GCAGGT
BsrGI 2975 TGTACA T^GTACA
4739 TGTACA
Bst1107I 2860 GTATAC GTA^TAC
3760 GTATAC
BstAPI 182 GCAGTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4406 GCAAGCTCTGC
BstXI 4223 CCATAACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4598 CCAAAGTGCTGG
BstZ17I 2860 GTATAC GTA^TAC
3760 GTATAC
EcoT14I 463 CCAAGG C^C[AT][AT]GG
5062 CCAAGG
EcoT22I 3524 ATGCAT ATGCA^T
5342 ATGCAT
HgaI 1349 GACGC GACGC(5/10)
1851 GACGC
NdeI 307 CATATG CA^TATG
2327 CATATG
NsiI 3524 ATGCAT ATGCA^T
5342 ATGCAT
NspI 536 GCATGT [GA]CATG^[CT]
677 ACATGC
PflMI 1626 CCACAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5062 CCAAGGGATGG
PpuMI 3581 AGGTCCC [GA]G^G[AT]CC[CT]
4694 GGGTCCT
ScaI 1251 AGTACT AGT^ACT
2820 AGTACT
SmaI 70 CCCGGG CCC^GGG
730 CCCGGG
SspBI 2975 TGTACA T^GTACA
4739 TGTACA
StyI 463 CCAAGG C^C[AT][AT]GG
5062 CCAAGG
Van91I 1626 CCACAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5062 CCAAGGGATGG
XcmI 682 CCACAGAGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1257 CCAGAAGGAAAATGG
XmaCI 70 CCCGGG C^CCGGG
730 CCCGGG
XmaI 70 CCCGGG C^CCGGG
730 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwNI
ApoI Asp700I AspHI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BsrDI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI