################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AatII Acc65I AclI AflII Aor51HI AscI Asp700I Asp718I AviII AvrII BbeI BfrI BlnI BsaBI BsiWI BsmBI BspDI BssHII BstBI ClaI CpoI Eco47III FseI FspI HindIII KasI KpnI MamI MfeI MluI MunI NaeI NarI NgoMIV NruI NspV PaeR7I PmeI Psp1406I PvuI RsrII SalI SbfI ScaI SfoI SfuI SnaBI SpeI Sse8387I SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 5969 GTATAC GT^[AC][GT]AC AccIII 2201 TCCGGA T^CCGGA AseI 4534 ATTAAT AT^TAAT AsnI 4534 ATTAAT AT^TAAT BamHI 3121 GGATCC G^GATCC BclI 1148 TGATCA T^GATCA BglII 3596 AGATCT A^GATCT BseAI 2201 TCCGGA T^CCGGA BsiEI 363 CGGCCG CG[GA][CT]^CG Bsp1407I 5915 TGTACA T^GTACA BspEI 2201 TCCGGA T^CCGGA BspLU11I 6157 ACATGT A^CATGT BsrGI 5915 TGTACA T^GTACA BssSI 1436 CACGAG CACGAG(-5/-1) Bst1107I 5969 GTATAC GTA^TAC BstZ17I 5969 GTATAC GTA^TAC DraIII 1436 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3026 GACTGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 363 CGGCCG C^GGCCG EclXI 363 CGGCCG C^GGCCG Eco52I 363 CGGCCG C^GGCCG EcoRV 6060 GATATC GAT^ATC FbaI 1148 TGATCA T^GATCA HpaI 2940 GTTAAC GTT^AAC KspI 4522 CCGCGG CCGC^GG MroI 2201 TCCGGA T^CCGGA NheI 152 GCTAGC G^CTAGC NotI 362 GCGGCCGC GC^GGCCGC PacI 6219 TTAATTAA TTAAT^TAA PshAI 5185 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4534 ATTAAT AT^TAAT SacII 4522 CCGCGG CCGC^GG SapI 4028 GCTCTTC GCTCTTC(1/4) SexAI 632 ACCTGGT A^CC[AT]GGT SfiI 390 GGCCAGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 6104 CACCGGTG C[GA]^CCGG[CT]G SspBI 5915 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AcyI 427 GACGCC G[GA]^CG[CT]C 1579 GACGCC AflIII 4079 ACACGT A^C[GA][CT]GT 6157 ACATGT AgeI 5481 ACCGGT A^CCGGT 6105 ACCGGT AhdI 2359 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2422 GACCCATAGTC AspEI 2359 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2422 GACCCATAGTC AspI 2717 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4614 GACAGAGTC BbrPI 199 CACGTG CAC^GTG 3266 CACGTG BcgI 663 CGACTTGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4212 CGATCTTTTTGC BciVI 907 GGATAC GTATCC(6/5) 5585 GGATAC BsaHI 427 GACGCC G[GA]^CG[CT]C 1579 GACGCC BsmI 2889 GAATGC GAATGC(1/-1) 3288 GCATTC BspHI 1151 TCATGA T^CATGA 4132 TCATGA BsrFI 5481 ACCGGT [GA]^CCGG[CT] 6105 ACCGGT BstEII 1803 GGTCACC G^GT[ACGT]ACC 4789 GGTCACC BstPI 1803 GGTCACC G^GT[ACGT]ACC 4789 GGTCACC Cfr10I 5481 ACCGGT [GA]^CCGG[CT] 6105 ACCGGT Eam1105I 2359 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2422 GACCCATAGTC EcoO65I 1803 GGTCACC G^GT[ACGT]ACC 4789 GGTCACC EcoT22I 2214 ATGCAT ATGCA^T 4970 ATGCAT HaeII 272 AGCGCC [GA]GCGC^[CT] 4266 AGCGCC Hin1I 427 GACGCC G[GA]^CG[CT]C 1579 GACGCC NdeI 698 CATATG CA^TATG 5260 CATATG NsiI 2214 ATGCAT ATGCA^T 4970 ATGCAT PflFI 2717 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4614 GACAGAGTC PinAI 5481 ACCGGT A^CCGGT 6105 ACCGGT PmaCI 199 CACGTG CAC^GTG 3266 CACGTG PmlI 199 CACGTG CAC^GTG 3266 CACGTG RcaI 1151 TCATGA T^CATGA 4132 TCATGA SmaI 64 CCCGGG CCC^GGG 3125 CCCGGG SphI 987 GCATGC GCATG^C 4294 GCATGC Tth111I 2717 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4614 GACAGAGTC XbaI 3948 TCTAGA T^CTAGA 4939 TCTAGA XmaCI 64 CCCGGG C^CCGGG 3125 CCCGGG XmaI 64 CCCGGG C^CCGGG 3125 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AccII AciI AcsI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII