##################################
# NO CUT ENZYMES (   53 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       Aor51HI     
AscI        Asp700I     Asp718I     AviII       AvrII       
BbeI        BfrI        BlnI        BsaBI       BsiWI       
BsmBI       BspDI       BssHII      BstBI       ClaI        
CpoI        Eco47III    FseI        FspI        HindIII     
KasI        KpnI        MamI        MfeI        MluI        
MunI        NaeI        NarI        NgoMIV      NruI        
NspV        PaeR7I      PmeI        Psp1406I    PvuI        
RsrII       SalI        SbfI        ScaI        SfoI        
SfuI        SnaBI       SpeI        Sse8387I    SspI        
SwaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI         5969  GTATAC               GT^[AC][GT]AC
AccIII       2201  TCCGGA               T^CCGGA
AseI         4534  ATTAAT               AT^TAAT
AsnI         4534  ATTAAT               AT^TAAT
BamHI        3121  GGATCC               G^GATCC
BclI         1148  TGATCA               T^GATCA
BglII        3596  AGATCT               A^GATCT
BseAI        2201  TCCGGA               T^CCGGA
BsiEI         363  CGGCCG               CG[GA][CT]^CG
Bsp1407I     5915  TGTACA               T^GTACA
BspEI        2201  TCCGGA               T^CCGGA
BspLU11I     6157  ACATGT               A^CATGT
BsrGI        5915  TGTACA               T^GTACA
BssSI        1436  CACGAG               CACGAG(-5/-1)
Bst1107I     5969  GTATAC               GTA^TAC
BstZ17I      5969  GTATAC               GTA^TAC
DraIII       1436  CACGAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         3026  GACTGAGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          363  CGGCCG               C^GGCCG
EclXI         363  CGGCCG               C^GGCCG
Eco52I        363  CGGCCG               C^GGCCG
EcoRV        6060  GATATC               GAT^ATC
FbaI         1148  TGATCA               T^GATCA
HpaI         2940  GTTAAC               GTT^AAC
KspI         4522  CCGCGG               CCGC^GG
MroI         2201  TCCGGA               T^CCGGA
NheI          152  GCTAGC               G^CTAGC
NotI          362  GCGGCCGC             GC^GGCCGC
PacI         6219  TTAATTAA             TTAAT^TAA
PshAI        5185  GACCCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        4534  ATTAAT               AT^TAAT
SacII        4522  CCGCGG               CCGC^GG
SapI         4028  GCTCTTC              GCTCTTC(1/4)
SexAI         632  ACCTGGT              A^CC[AT]GGT
SfiI          390  GGCCAGCGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI        6104  CACCGGTG             C[GA]^CCGG[CT]G
SspBI        5915  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AcyI          427  GACGCC               G[GA]^CG[CT]C
             1579  GACGCC              
AflIII       4079  ACACGT               A^C[GA][CT]GT
             6157  ACATGT              
AgeI         5481  ACCGGT               A^CCGGT
             6105  ACCGGT              
AhdI         2359  GACTCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2422  GACCCATAGTC         
AspEI        2359  GACTCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2422  GACCCATAGTC         
AspI         2717  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4614  GACAGAGTC           
BbrPI         199  CACGTG               CAC^GTG
             3266  CACGTG              
BcgI          663  CGACTTGCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4212  CGATCTTTTTGC        
BciVI         907  GGATAC               GTATCC(6/5)
             5585  GGATAC              
BsaHI         427  GACGCC               G[GA]^CG[CT]C
             1579  GACGCC              
BsmI         2889  GAATGC               GAATGC(1/-1)
             3288  GCATTC              
BspHI        1151  TCATGA               T^CATGA
             4132  TCATGA              
BsrFI        5481  ACCGGT               [GA]^CCGG[CT]
             6105  ACCGGT              
BstEII       1803  GGTCACC              G^GT[ACGT]ACC
             4789  GGTCACC             
BstPI        1803  GGTCACC              G^GT[ACGT]ACC
             4789  GGTCACC             
Cfr10I       5481  ACCGGT               [GA]^CCGG[CT]
             6105  ACCGGT              
Eam1105I     2359  GACTCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2422  GACCCATAGTC         
EcoO65I      1803  GGTCACC              G^GT[ACGT]ACC
             4789  GGTCACC             
EcoT22I      2214  ATGCAT               ATGCA^T
             4970  ATGCAT              
HaeII         272  AGCGCC               [GA]GCGC^[CT]
             4266  AGCGCC              
Hin1I         427  GACGCC               G[GA]^CG[CT]C
             1579  GACGCC              
NdeI          698  CATATG               CA^TATG
             5260  CATATG              
NsiI         2214  ATGCAT               ATGCA^T
             4970  ATGCAT              
PflFI        2717  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4614  GACAGAGTC           
PinAI        5481  ACCGGT               A^CCGGT
             6105  ACCGGT              
PmaCI         199  CACGTG               CAC^GTG
             3266  CACGTG              
PmlI          199  CACGTG               CAC^GTG
             3266  CACGTG              
RcaI         1151  TCATGA               T^CATGA
             4132  TCATGA              
SmaI           64  CCCGGG               CCC^GGG
             3125  CCCGGG              
SphI          987  GCATGC               GCATG^C
             4294  GCATGC              
Tth111I      2717  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4614  GACAGAGTC           
XbaI         3948  TCTAGA               T^CTAGA
             4939  TCTAGA              
XmaCI          64  CCCGGG               C^CCGGG
             3125  CCCGGG              
XmaI           64  CCCGGG               C^CCGGG
             3125  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  134 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
Alw44I      AlwI        AlwNI       AocI        ApaI        
ApaLI       ApoI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRI       EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII