##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BciVI BfrI BglI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BsrBI
BsrDI BsrGI BssHII BssSI BstBI
BstEII BstPI BstXI ClaI CpoI
DraIII DrdI Eam1105I Eco47III EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HaeII HpaI KasI KpnI KspI
MamI MluI MluNI MroI MscI
NarI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SspBI SspI
Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 2278 CGCG CG^CG
AcyI 1561 GACGCC G[GA]^CG[CT]C
AocI 796 CCTAAGG CC^T[ACGT]AGG
BamHI 419 GGATCC G^GATCC
BcgI 1935 CGATTACAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2016 TGATCA T^GATCA
BlpI 1287 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1287 GCTGAGC GC^T[ACGT]AGC
BsaHI 1561 GACGCC G[GA]^CG[CT]C
BsaI 1763 GAGACC GGTCTC(1/5)
BspHI 242 TCATGA T^CATGA
BsrFI 1578 GCCGGC [GA]^CCGG[CT]
Bst1107I 1319 GTATAC GTA^TAC
BstAPI 1569 GCACTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 303 GGATG GGATG(2/0)
BstUI 2278 CGCG CG^CG
BstZ17I 1319 GTATAC GTA^TAC
Bsu36I 796 CCTAAGG CC^T[ACGT]AGG
CelII 1287 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1578 GCCGGC [GA]^CCGG[CT]
EagI 1580 CGGCCG C^GGCCG
EarI 276 CTCTTC CTCTTC(1/4)
EclXI 1580 CGGCCG C^GGCCG
Eco52I 1580 CGGCCG C^GGCCG
Eco81I 796 CCTAAGG CC^T[ACGT]AGG
EcoRI 238 GAATTC G^AATTC
EcoT14I 1553 CCATGG C^C[AT][AT]GG
FauI 2160 CCCGC CCCGC(4/6)
FbaI 2016 TGATCA T^GATCA
FokI 303 GGATG GGATG(9/13)
HgaI 1561 GACGC GACGC(5/10)
Hin1I 1561 GACGCC G[GA]^CG[CT]C
HincII 661 GTCAAC GT[CT]^[GA]AC
HindII 661 GTCAAC GT[CT]^[GA]AC
Ksp632I 276 CTCTTC CTCTTC(1/4)
MaeII 119 ACGT A^CGT
MvnI 2278 CGCG CG^CG
NaeI 1578 GCCGGC GCC^GGC
NcoI 1553 CCATGG C^CATGG
NgoMIV 1578 GCCGGC G^CCGGC
NheI 268 GCTAGC G^CTAGC
PaeR7I 2349 CTCGAG C^TCGAG
PflMI 2128 CCAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 31 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 242 TCATGA T^CATGA
SmaI 2259 CCCGGG CCC^GGG
SpeI 2424 ACTAGT A^CTAGT
StuI 1231 AGGCCT AGG^CCT
StyI 1553 CCATGG C^C[AT][AT]GG
SwaI 1166 ATTTAAAT ATTT^AAAT
Van91I 2128 CCAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 127 CCAGGACCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2349 CTCGAG C^TCGAG
XmaCI 2259 CCCGGG C^CCGGG
XmaI 2259 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1319 GTATAC GT^[AC][GT]AC
2031 GTCTAC
ApaI 1720 GGGCCC GGGCC^C
2156 GGGCCC
AspHI 850 GAGCTC G[AT]GC[AT]^C
1470 GAGCTC
AvaI 2259 CCCGGG C^[CT]CG[GA]G
2349 CTCGAG
BsiEI 1580 CGGCCG CG[GA][CT]^CG
2237 CGGTCG
BsiHKAI 850 GAGCTC G[AT]GC[AT]^C
1470 GAGCTC
BsmI 426 GAATGC GAATGC(1/-1)
2322 GAATGC
BsoBI 2259 CCCGGG C^[CT]CG[GA]G
2349 CTCGAG
BspMI 665 ACCTGC ACCTGC(4/8)
1377 ACCTGC
BstYI 419 GGATCC [GA]^GATC[CT]
1113 AGATCC
CfoI 1910 GCGC GCG^C
2279 GCGC
DraI 1167 TTTAAA TTT^AAA
2387 TTTAAA
DsaI 1553 CCATGG C^C[GA][CT]GG
2204 CCACGG
EaeI 521 CGGCCA [CT]^GGCC[GA]
1580 CGGCCG
HhaI 1910 GCGC GCG^C
2279 GCGC
HinP1I 1910 GCGC G^CGC
2279 GCGC
MfeI 333 CAATTG C^AATTG
731 CAATTG
MflI 419 GGATCC [GA]^GATC[CT]
1113 AGATCC
MslI 228 CATTCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1662 CATGACCGTG
MunI 333 CAATTG C^AATTG
731 CAATTG
NdeI 356 CATATG CA^TATG
2298 CATATG
PpuMI 29 GGGACCT [GA]G^G[AT]CC[CT]
129 AGGACCC
PstI 667 CTGCAG CTGCA^G
1991 CTGCAG
SacI 850 GAGCTC GAGCT^C
1470 GAGCTC
SfaNI 610 GATGC GCATC(5/9)
1354 GATGC
SfcI 667 CTGCAG C^T[GA][CT]AG
1991 CTGCAG
SmlI 1227 CTCAAG C^T[CT][GA]AG
2349 CTCGAG
XbaI 1060 TCTAGA T^CTAGA
2341 TCTAGA
XhoII 419 GGATCC [GA]^GATC[CT]
1113 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI HaeIII HapII HindIII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I