##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
AocI AseI AsnI AspEI AvrII
BciVI BclI BfrI BglII BlnI
BseAI BsiWI BspDI BspEI BspLU11I
Bst1107I BstBI BstZ17I Bsu36I ClaI
DraI Eam1105I Eco81I EcoRI EcoT22I
FbaI FseI HpaI MfeI MroI
MseI MunI NdeI NotI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I SalI SfuI SnaBI
SpeI SphI SspI SwaI Tru9I
XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1586 GACGTC GACGT^C
Acc65I 3228 GGTACC G^GTACC
AccI 1490 GTCTAC GT^[AC][GT]AC
AcsI 4145 AAATTC [GA]^AATT[CT]
ApoI 4145 AAATTC [GA]^AATT[CT]
Asp700I 459 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3228 GGTACC G^GTACC
AviII 3594 TGCGCA TGC^GCA
BamHI 2802 GGATCC G^GATCC
BcgI 5617 CGAGGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 5770 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 5533 ACCGGA [AT]^CCGG[AT]
BsmBI 836 CGTCTC CGTCTC(1/5)
BsmI 2885 GCATTC GAATGC(1/-1)
Bsp1407I 479 TGTACA T^GTACA
BsrGI 479 TGTACA T^GTACA
BstEII 1438 GGTGACC G^GT[ACGT]ACC
BstPI 1438 GGTGACC G^GT[ACGT]ACC
CpoI 5949 CGGACCG CG^G[AT]CCG
DrdI 674 GACCCGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1438 GGTGACC G^GT[ACGT]ACC
EcoRV 349 GATATC GAT^ATC
FspI 3594 TGCGCA TGC^GCA
HincII 3176 GTCAAC GT[CT]^[GA]AC
HindII 3176 GTCAAC GT[CT]^[GA]AC
HindIII 1257 AAGCTT A^AGCTT
KpnI 3228 GGTACC GGTAC^C
MamI 5770 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1515 ACGCGT A^CGCGT
NruI 1210 TCGCGA TCG^CGA
PaeR7I 3344 CTCGAG C^TCGAG
PpuMI 5247 GGGACCC [GA]G^G[AT]CC[CT]
RsrII 5949 CGGACCG CG^G[AT]CCG
SapI 2449 GCTCTTC GCTCTTC(1/4)
SbfI 952 CCTGCAGG CCTGCA^GG
SgrAI 1234 CGCCGGTG C[GA]^CCGG[CT]G
Sse8387I 952 CCTGCAGG CCTGCA^GG
SspBI 479 TGTACA T^GTACA
Tsp509I 4146 AATT ^AATT
XhoI 3344 CTCGAG C^TCGAG
XmnI 459 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflIII 1515 ACGCGT A^C[GA][CT]GT
4761 ACGTGT
Aor51HI 1992 AGCGCT AGC^GCT
2115 AGCGCT
AscI 1276 GGCGCGCC GG^CGCGCC
2230 GGCGCGCC
BbrPI 704 CACGTG CAC^GTG
1817 CACGTG
BspHI 3771 TCATGA T^CATGA
4782 TCATGA
BstXI 3535 CCAGGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5253 CCACGGAACTGG
Eco47III 1992 AGCGCT AGC^GCT
2115 AGCGCT
KspI 5454 CCGCGG CCGC^GG
5505 CCGCGG
PmaCI 704 CACGTG CAC^GTG
1817 CACGTG
PmlI 704 CACGTG CAC^GTG
1817 CACGTG
PvuI 263 CGATCG CGAT^CG
5178 CGATCG
RcaI 3771 TCATGA T^CATGA
4782 TCATGA
SacII 5454 CCGCGG CCGC^GG
5505 CCGCGG
ScaI 3717 AGTACT AGT^ACT
4020 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AspI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssHII BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NheI NlaIII NlaIV
NspI PflFI PflMI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfiI
SfoI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmaCI XmaI