##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Aor51HI ApaI AscI AspEI AvrII
BalI BamHI BbeI BbrPI BbvCI
BglI BlnI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstPI
Cfr10I ClaI CpoI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FseI HpaI KasI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NgoMIV NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SphI Sse8387I StuI
SwaI XcmI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 3035 GGTACC G^GTACC
AccII 75 CGCG CG^CG
AcyI 133 GACGCC G[GA]^CG[CT]C
AocI 236 CCTCAGG CC^T[ACGT]AGG
AseI 2133 ATTAAT AT^TAAT
AsnI 2133 ATTAAT AT^TAAT
Asp718I 3035 GGTACC G^GTACC
AspI 5502 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4816 TGCGCA TGC^GCA
BanI 3035 GGTACC G^G[CT][GA]CC
BcgI 401 CGAAAAATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 471 TGATCA T^GATCA
BglII 4966 AGATCT A^GATCT
BlpI 3301 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3301 GCTCAGC GC^T[ACGT]AGC
BsaAI 3538 TACGTA [CT]AC^GT[GA]
BsaHI 133 GACGCC G[GA]^CG[CT]C
BsaI 1838 GGTCTC GGTCTC(1/5)
BstUI 75 CGCG CG^CG
BstXI 5497 CCAAAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 236 CCTCAGG CC^T[ACGT]AGG
CelII 3301 GCTCAGC GC^T[ACGT]AGC
DraII 1244 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1822 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 236 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1244 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 2745 GAATTC G^AATTC
FbaI 471 TGATCA T^GATCA
FspI 4816 TGCGCA TGC^GCA
HgaI 133 GACGC GACGC(5/10)
Hin1I 133 GACGCC G[GA]^CG[CT]C
HincII 5425 GTTGAC GT[CT]^[GA]AC
HindII 5425 GTTGAC GT[CT]^[GA]AC
KpnI 3035 GGTACC GGTAC^C
KspI 74 CCGCGG CCGC^GG
MvnI 75 CGCG CG^CG
NcoI 3926 CCATGG C^CATGG
NheI 3669 GCTAGC G^CTAGC
PflFI 5502 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 5139 CCAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1244 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 2133 ATTAAT AT^TAAT
PstI 4581 CTGCAG CTGCA^G
PvuII 4366 CAGCTG CAG^CTG
SacI 5682 GAGCTC GAGCT^C
SacII 74 CCGCGG CCGC^GG
SmaI 160 CCCGGG CCC^GGG
SnaBI 3538 TACGTA TAC^GTA
SpeI 229 ACTAGT A^CTAGT
Tth111I 5502 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 5139 CCAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 160 CCCGGG C^CCGGG
XmaI 160 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Alw44I 409 GTGCAC G^TGCAC
1792 GTGCAC
ApaLI 409 GTGCAC G^TGCAC
1792 GTGCAC
Asp700I 227 GAACTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1743 GAAACTTTTC
BanII 53 GAGCCC G[GA]GC[CT]^C
5682 GAGCTC
BciVI 3890 GGATAC GTATCC(6/5)
242 GTATCC
BsaBI 3318 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5725 GATAAAGATC
BsgI 432 CTGCAC GTGCAG(16/14)
3374 CTGCAC
BsmI 4262 GAATGC GAATGC(1/-1)
5491 GCATTC
BsrBI 155 CCGCTC CCGCTC(-3/-3)
139 GAGCGG
Bst1107I 2492 GTATAC GTA^TAC
3056 GTATAC
BstZ17I 2492 GTATAC GTA^TAC
3056 GTATAC
DrdI 2231 GACTTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5281 GACTGATGGGTC
DsaI 74 CCGCGG C^C[GA][CT]GG
3926 CCATGG
EcoNI 704 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2387 CCTCTTATAGG
EcoRV 3001 GATATC GAT^ATC
3598 GATATC
EcoT22I 2730 ATGCAT ATGCA^T
3705 ATGCAT
HaeII 86 GGCGCT [GA]GCGC^[CT]
96 GGCGCT
MamI 3318 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5725 GATAAAGATC
NsiI 2730 ATGCAT ATGCA^T
3705 ATGCAT
PaeR7I 1420 CTCGAG C^TCGAG
1797 CTCGAG
ScaI 5372 AGTACT AGT^ACT
5692 AGTACT
XhoI 1420 CTCGAG C^TCGAG
1797 CTCGAG
XmnI 227 GAACTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1743 GAAACTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BbsI BbvI
BcnI BfaI BfrI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspHI BspLU11I BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI PleI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII