################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AccI AccIII AclI AgeI Aor51HI ApaI AscI AseI AsnI AspI AviII AvrII BglI BlnI BseAI BsiWI BspDI BspEI BspLU11I BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI Eco47III EcoRV EcoT22I FseI FspI HpaI KspI MluI MroI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I Van91I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 1703 GGTACC G^GTACC AflII 3946 CTTAAG C^TTAAG AflIII 3445 ACACGT A^C[GA][CT]GT Alw44I 2123 GTGCAC G^TGCAC AocI 4645 CCTAAGG CC^T[ACGT]AGG ApaLI 2123 GTGCAC G^TGCAC Asp718I 1703 GGTACC G^GTACC BbrPI 462 CACGTG CAC^GTG BbvCI 5329 GCTGAGG CCTCAGC(-5/-2) BciVI 3646 GTATCC GTATCC(6/5) BfrI 3946 CTTAAG C^TTAAG BsaBI 2170 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 1725 CGGCCG CG[GA][CT]^CG Bsp1407I 1807 TGTACA T^GTACA BsrBI 469 GAGCGG CCGCTC(-3/-3) BsrGI 1807 TGTACA T^GTACA BstEII 5334 GGTGACC G^GT[ACGT]ACC BstPI 5334 GGTGACC G^GT[ACGT]ACC Bsu36I 4645 CCTAAGG CC^T[ACGT]AGG DraI 1519 TTTAAA TTT^AAA DrdI 5594 GACATAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1725 CGGCCG C^GGCCG EclXI 1725 CGGCCG C^GGCCG Eco52I 1725 CGGCCG C^GGCCG Eco81I 4645 CCTAAGG CC^T[ACGT]AGG EcoO65I 5334 GGTGACC G^GT[ACGT]ACC KpnI 1703 GGTACC GGTAC^C MamI 2170 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 657 CAATTG C^AATTG MunI 657 CAATTG C^AATTG NaeI 3455 GCCGGC GCC^GGC NdeI 1709 CATATG CA^TATG NgoMIV 3455 GCCGGC G^CCGGC NheI 3228 GCTAGC G^CTAGC PmaCI 462 CACGTG CAC^GTG PmlI 462 CACGTG CAC^GTG SapI 1228 GAAGAGC GCTCTTC(1/4) SexAI 2363 ACCTGGT A^CC[AT]GGT SphI 2942 GCATGC GCATG^C SspBI 1807 TGTACA T^GTACA XbaI 4501 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AatII 1277 GACGTC GACGT^C 5307 GACGTC BamHI 3029 GGATCC G^GATCC 5417 GGATCC BanII 3560 GAGCTC G[GA]GC[CT]^C 4807 GAGCTC BbeI 4721 GGCGCC GGCGC^C 5700 GGCGCC BcgI 4947 CGAAAGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5173 GCAGGCCTTTCG BclI 2175 TGATCA T^GATCA 2714 TGATCA BcnI 402 CCGGG CC^[GC]GG 5392 CCCGG BglII 246 AGATCT A^GATCT 2990 AGATCT BlpI 4603 GCTCAGC GC^T[ACGT]AGC 4804 GCTGAGC Bpu1102I 4603 GCTCAGC GC^T[ACGT]AGC 4804 GCTGAGC BsaAI 386 CACGTA [CT]AC^GT[GA] 462 CACGTG BsaWI 368 ACCGGA [AT]^CCGG[AT] 2761 TCCGGT BsmI 3794 GAATGC GAATGC(1/-1) 5547 GAATGC BspHI 4614 TCATGA T^CATGA 5291 TCATGA BsrDI 1564 GCAATG GCAATG(2/0) 4529 GCAATG CelII 4603 GCTCAGC GC^T[ACGT]AGC 4804 GCTGAGC DraIII 1096 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG 1633 CACAGGGTG FbaI 2175 TGATCA T^GATCA 2714 TGATCA HgaI 2597 GACGC GACGC(5/10) 2490 GCGTC HincII 364 GTTGAC GT[CT]^[GA]AC 2752 GTCAAC HindII 364 GTTGAC GT[CT]^[GA]AC 2752 GTCAAC KasI 4721 GGCGCC G^GCGCC 5700 GGCGCC NarI 4721 GGCGCC GG^CGCC 5700 GGCGCC NciI 402 CCGGG CC^[GC]GG 5392 CCCGG PaeR7I 2741 CTCGAG C^TCGAG 3545 CTCGAG PpuMI 931 GGGTCCT [GA]G^G[AT]CC[CT] 2924 AGGTCCT PstI 2640 CTGCAG CTGCA^G 3059 CTGCAG RcaI 4614 TCATGA T^CATGA 5291 TCATGA SacI 3560 GAGCTC GAGCT^C 4807 GAGCTC SfoI 4721 GGCGCC GGC^GCC 5700 GGCGCC SspI 1000 AATATT AAT^ATT 1361 AATATT XhoI 2741 CTCGAG C^TCGAG 3545 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AhdI AluI AlwI AlwNI ApoI Asp700I AspEI AspHI AvaI AvaII BalI BanI BbsI BbvI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI