##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AccI        AccIII      AclI        AgeI        Aor51HI     
ApaI        AscI        AseI        AsnI        AspI        
AviII       AvrII       BglI        BlnI        BseAI       
BsiWI       BspDI       BspEI       BspLU11I    BssHII      
BssSI       Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        Eco47III    EcoRV       EcoT22I     FseI        
FspI        HpaI        KspI        MluI        MroI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PflMI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SwaI        Tth111I     Van91I      XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I       1703  GGTACC               G^GTACC
AflII        3946  CTTAAG               C^TTAAG
AflIII       3445  ACACGT               A^C[GA][CT]GT
Alw44I       2123  GTGCAC               G^TGCAC
AocI         4645  CCTAAGG              CC^T[ACGT]AGG
ApaLI        2123  GTGCAC               G^TGCAC
Asp718I      1703  GGTACC               G^GTACC
BbrPI         462  CACGTG               CAC^GTG
BbvCI        5329  GCTGAGG              CCTCAGC(-5/-2)
BciVI        3646  GTATCC               GTATCC(6/5)
BfrI         3946  CTTAAG               C^TTAAG
BsaBI        2170  GATATTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        1725  CGGCCG               CG[GA][CT]^CG
Bsp1407I     1807  TGTACA               T^GTACA
BsrBI         469  GAGCGG               CCGCTC(-3/-3)
BsrGI        1807  TGTACA               T^GTACA
BstEII       5334  GGTGACC              G^GT[ACGT]ACC
BstPI        5334  GGTGACC              G^GT[ACGT]ACC
Bsu36I       4645  CCTAAGG              CC^T[ACGT]AGG
DraI         1519  TTTAAA               TTT^AAA
DrdI         5594  GACATAGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1725  CGGCCG               C^GGCCG
EclXI        1725  CGGCCG               C^GGCCG
Eco52I       1725  CGGCCG               C^GGCCG
Eco81I       4645  CCTAAGG              CC^T[ACGT]AGG
EcoO65I      5334  GGTGACC              G^GT[ACGT]ACC
KpnI         1703  GGTACC               GGTAC^C
MamI         2170  GATATTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          657  CAATTG               C^AATTG
MunI          657  CAATTG               C^AATTG
NaeI         3455  GCCGGC               GCC^GGC
NdeI         1709  CATATG               CA^TATG
NgoMIV       3455  GCCGGC               G^CCGGC
NheI         3228  GCTAGC               G^CTAGC
PmaCI         462  CACGTG               CAC^GTG
PmlI          462  CACGTG               CAC^GTG
SapI         1228  GAAGAGC              GCTCTTC(1/4)
SexAI        2363  ACCTGGT              A^CC[AT]GGT
SphI         2942  GCATGC               GCATG^C
SspBI        1807  TGTACA               T^GTACA
XbaI         4501  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AatII        1277  GACGTC               GACGT^C
             5307  GACGTC              
BamHI        3029  GGATCC               G^GATCC
             5417  GGATCC              
BanII        3560  GAGCTC               G[GA]GC[CT]^C
             4807  GAGCTC              
BbeI         4721  GGCGCC               GGCGC^C
             5700  GGCGCC              
BcgI         4947  CGAAAGAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             5173  GCAGGCCTTTCG        
BclI         2175  TGATCA               T^GATCA
             2714  TGATCA              
BcnI          402  CCGGG                CC^[GC]GG
             5392  CCCGG               
BglII         246  AGATCT               A^GATCT
             2990  AGATCT              
BlpI         4603  GCTCAGC              GC^T[ACGT]AGC
             4804  GCTGAGC             
Bpu1102I     4603  GCTCAGC              GC^T[ACGT]AGC
             4804  GCTGAGC             
BsaAI         386  CACGTA               [CT]AC^GT[GA]
              462  CACGTG              
BsaWI         368  ACCGGA               [AT]^CCGG[AT]
             2761  TCCGGT              
BsmI         3794  GAATGC               GAATGC(1/-1)
             5547  GAATGC              
BspHI        4614  TCATGA               T^CATGA
             5291  TCATGA              
BsrDI        1564  GCAATG               GCAATG(2/0)
             4529  GCAATG              
CelII        4603  GCTCAGC              GC^T[ACGT]AGC
             4804  GCTGAGC             
DraIII       1096  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1633  CACAGGGTG           
FbaI         2175  TGATCA               T^GATCA
             2714  TGATCA              
HgaI         2597  GACGC                GACGC(5/10)
             2490  GCGTC               
HincII        364  GTTGAC               GT[CT]^[GA]AC
             2752  GTCAAC              
HindII        364  GTTGAC               GT[CT]^[GA]AC
             2752  GTCAAC              
KasI         4721  GGCGCC               G^GCGCC
             5700  GGCGCC              
NarI         4721  GGCGCC               GG^CGCC
             5700  GGCGCC              
NciI          402  CCGGG                CC^[GC]GG
             5392  CCCGG               
PaeR7I       2741  CTCGAG               C^TCGAG
             3545  CTCGAG              
PpuMI         931  GGGTCCT              [GA]G^G[AT]CC[CT]
             2924  AGGTCCT             
PstI         2640  CTGCAG               CTGCA^G
             3059  CTGCAG              
RcaI         4614  TCATGA               T^CATGA
             5291  TCATGA              
SacI         3560  GAGCTC               GAGCT^C
             4807  GAGCTC              
SfoI         4721  GGCGCC               GGC^GCC
             5700  GGCGCC              
SspI         1000  AATATT               AAT^ATT
             1361  AATATT              
XhoI         2741  CTCGAG               C^TCGAG
             3545  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AhdI        AluI        AlwI        AlwNI       ApoI        
Asp700I     AspEI       AspHI       AvaI        AvaII       
BalI        BanI        BbsI        BbvI        BfaI        
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
Eam1105I    EarI        EcoNI       EcoO109I    EcoRI       
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI