##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AflII Aor51HI AscI
AseI AsnI Asp718I AspI AviII
AvrII BbeI BcgI BfrI BlnI
BsaBI BsiEI BsiWI BspDI BspHI
BspLU11I BssHII Bst1107I BstZ17I ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoRV EcoT22I FseI FspI HaeII
HpaI KasI KpnI KspI MamI
MluI NarI NheI NotI NruI
NsiI PacI PaeR7I PflFI PmeI
PshAI PshBI PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 2174 GTCTAC GT^[AC][GT]AC
AccII 4240 CGCG CG^CG
AccIII 5105 TCCGGA T^CCGGA
AclI 2706 AACGTT AA^CGTT
AflIII 2533 ACACGT A^C[GA][CT]GT
AgeI 3678 ACCGGT A^CCGGT
AhdI 1015 GACCATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1015 GACCATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 376 GGTGCC G^G[CT][GA]CC
BclI 1886 TGATCA T^GATCA
BseAI 5105 TCCGGA T^CCGGA
BsmI 406 GAATGC GAATGC(1/-1)
Bsp1407I 5228 TGTACA T^GTACA
BspEI 5105 TCCGGA T^CCGGA
BsrDI 2416 GCAATG GCAATG(2/0)
BsrGI 5228 TGTACA T^GTACA
BssSI 4650 CACGAG CACGAG(-5/-1)
BstBI 2734 TTCGAA TT^CGAA
BstUI 4240 CGCG CG^CG
BstXI 1584 CCAGCTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 4081 TTTAAA TTT^AAA
DrdI 4832 GACCCTTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1015 GACCATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3904 GAATTC G^AATTC
FbaI 1886 TGATCA T^GATCA
HindIII 2818 AAGCTT A^AGCTT
MfeI 3638 CAATTG C^AATTG
MroI 5105 TCCGGA T^CCGGA
MunI 3638 CAATTG C^AATTG
MvnI 4240 CGCG CG^CG
NdeI 2498 CATATG CA^TATG
NspV 2734 TTCGAA TT^CGAA
PinAI 3678 ACCGGT A^CCGGT
Psp1406I 2706 AACGTT AA^CGTT
SexAI 689 ACCTGGT A^CC[AT]GGT
SfuI 2734 TTCGAA TT^CGAA
SmaI 45 CCCGGG CCC^GGG
SspBI 5228 TGTACA T^GTACA
SspI 1985 AATATT AAT^ATT
XmaCI 45 CCCGGG C^CCGGG
XmaI 45 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 2292 CCTCAGG CC^T[ACGT]AGG
4249 CCTGAGG
Asp700I 585 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4671 GAAGCTGTTC
BbrPI 2809 CACGTG CAC^GTG
5210 CACGTG
BciVI 1488 GGATAC GTATCC(6/5)
3516 GGATAC
BglI 1690 GCCACTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1725 GCCAGAGCGGC
BsaAI 2809 CACGTG [CT]AC^GT[GA]
5210 CACGTG
BsmBI 419 GAGACG CGTCTC(1/5)
3018 GAGACG
BsrBI 647 CCGCTC CCGCTC(-3/-3)
1729 GAGCGG
BstEII 2937 GGTCACC G^GT[ACGT]ACC
3888 GGTGACC
BstPI 2937 GGTCACC G^GT[ACGT]ACC
3888 GGTGACC
Bsu36I 2292 CCTCAGG CC^T[ACGT]AGG
4249 CCTGAGG
Eco81I 2292 CCTCAGG CC^T[ACGT]AGG
4249 CCTGAGG
EcoO65I 2937 GGTCACC G^GT[ACGT]ACC
3888 GGTGACC
HincII 1542 GTCAAC GT[CT]^[GA]AC
3793 GTTGAC
HindII 1542 GTCAAC GT[CT]^[GA]AC
3793 GTTGAC
NspI 1608 GCATGT [GA]CATG^[CT]
2747 GCATGT
PmaCI 2809 CACGTG CAC^GTG
5210 CACGTG
PmlI 2809 CACGTG CAC^GTG
5210 CACGTG
StuI 1482 AGGCCT AGG^CCT
4430 AGGCCT
XmnI 585 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4671 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
ApoI AspHI AvaI AvaII BalI
BamHI BanII BbsI BbvCI BbvI
BcnI BfaI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII