##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccI AccIII AflIII
AgeI AhdI AseI AsnI Asp700I
Asp718I AspEI BbrPI BsaAI BseAI
BsiWI BspDI BspEI BspLU11I Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI Eam1105I EcoRV EcoT22I FseI
HpaI KpnI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SalI ScaI SexAI
SfuI SgrAI SnaBI SpeI SphI
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AclI 513 AACGTT AA^CGTT
AflII 1777 CTTAAG C^TTAAG
Alw44I 2015 GTGCAC G^TGCAC
Aor51HI 2447 AGCGCT AGC^GCT
ApaLI 2015 GTGCAC G^TGCAC
AscI 2647 GGCGCGCC GG^CGCGCC
AviII 5974 TGCGCA TGC^GCA
AvrII 3694 CCTAGG C^CTAGG
BbeI 876 GGCGCC GGCGC^C
BclI 2049 TGATCA T^GATCA
BfrI 1777 CTTAAG C^TTAAG
BlnI 3694 CCTAGG C^CTAGG
BsaBI 1839 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1253 TCCGGT [AT]^CCGG[AT]
BsiEI 2233 CGGCCG CG[GA][CT]^CG
BsmI 4636 GCATTC GAATGC(1/-1)
Bsp1407I 393 TGTACA T^GTACA
BspHI 3902 TCATGA T^CATGA
BsrDI 288 GCAATG GCAATG(2/0)
BsrGI 393 TGTACA T^GTACA
BssHII 2648 GCGCGC G^CGCGC
EagI 2233 CGGCCG C^GGCCG
EclXI 2233 CGGCCG C^GGCCG
Eco47III 2447 AGCGCT AGC^GCT
Eco52I 2233 CGGCCG C^GGCCG
EcoRI 1859 GAATTC G^AATTC
FbaI 2049 TGATCA T^GATCA
FspI 5974 TGCGCA TGC^GCA
HincII 818 GTCAAC GT[CT]^[GA]AC
HindII 818 GTCAAC GT[CT]^[GA]AC
HindIII 4324 AAGCTT A^AGCTT
KasI 876 GGCGCC G^GCGCC
MamI 1839 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 3685 GCCGGC GCC^GGC
NarI 876 GGCGCC GG^CGCC
NgoMIV 3685 GCCGGC G^CCGGC
NspI 4280 GCATGT [GA]CATG^[CT]
Psp1406I 513 AACGTT AA^CGTT
RcaI 3902 TCATGA T^CATGA
SacI 5520 GAGCTC GAGCT^C
SbfI 3584 CCTGCAGG CCTGCA^GG
SfiI 3160 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 876 GGCGCC GGC^GCC
Sse8387I 3584 CCTGCAGG CCTGCA^GG
SspBI 393 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 876 GGCGCC G[GA]^CG[CT]C
935 GGCGTC
AocI 208 CCTAAGG CC^T[ACGT]AGG
4781 CCTAAGG
AspI 2977 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3851 GACACTGTC
BciVI 3405 GGATAC GTATCC(6/5)
4648 GTATCC
BglII 268 AGATCT A^GATCT
2256 AGATCT
BsaHI 876 GGCGCC G[GA]^CG[CT]C
935 GGCGTC
BsmBI 2975 GAGACG CGTCTC(1/5)
5586 GAGACG
BsrBI 1198 CCGCTC CCGCTC(-3/-3)
5337 GAGCGG
BsrFI 623 ACCGGC [GA]^CCGG[CT]
3685 GCCGGC
BssSI 4602 CACGAG CACGAG(-5/-1)
782 CTCGTG
Bsu36I 208 CCTAAGG CC^T[ACGT]AGG
4781 CCTAAGG
Cfr10I 623 ACCGGC [GA]^CCGG[CT]
3685 GCCGGC
DraIII 2762 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3461 CACCAAGTG
Eco81I 208 CCTAAGG CC^T[ACGT]AGG
4781 CCTAAGG
Hin1I 876 GGCGCC G[GA]^CG[CT]C
935 GGCGTC
KspI 405 CCGCGG CCGC^GG
6013 CCGCGG
PflFI 2977 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3851 GACACTGTC
PvuII 598 CAGCTG CAG^CTG
4037 CAGCTG
SacII 405 CCGCGG CCGC^GG
6013 CCGCGG
SapI 1562 GAAGAGC GCTCTTC(1/4)
4012 GAAGAGC
SmaI 296 CCCGGG CCC^GGG
4405 CCCGGG
TaqI 483 TCGA T^CGA
5013 TCGA
Tth111I 2977 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3851 GACACTGTC
TthHB8I 483 TCGA T^CGA
5013 TCGA
XmaCI 296 CCCGGG C^CCGGG
4405 CCCGGG
XmaI 296 CCCGGG C^CCGGG
4405 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII