##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AflII AhdI
AscI Asp718I AspEI AviII AvrII
BbrPI BcgI BclI BfrI BlnI
BlpI Bpu1102I BsaBI BseAI BsiWI
BspDI BspEI BsrBI BssHII BssSI
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FbaI FseI
FspI HpaI KpnI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PmaCI PmeI PmlI RsrII SalI
SexAI SfiI SfuI SnaBI SphI
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AclI 4836 AACGTT AA^CGTT
AcyI 991 GGCGCC G[GA]^CG[CT]C
Aor51HI 3344 AGCGCT AGC^GCT
AseI 5938 ATTAAT AT^TAAT
AsnI 5938 ATTAAT AT^TAAT
BalI 2638 TGGCCA TGG^CCA
BamHI 1462 GGATCC G^GATCC
BbeI 991 GGCGCC GGCGC^C
BciVI 3802 GTATCC GTATCC(6/5)
BsaAI 4869 TACGTG [CT]AC^GT[GA]
BsaHI 991 GGCGCC G[GA]^CG[CT]C
BsiEI 2381 CGATCG CG[GA][CT]^CG
BspHI 4969 TCATGA T^CATGA
BspLU11I 3602 ACATGT A^CATGT
Eco47III 3344 AGCGCT AGC^GCT
Hin1I 991 GGCGCC G[GA]^CG[CT]C
HindIII 2874 AAGCTT A^AGCTT
KasI 991 GGCGCC G^GCGCC
KspI 3634 CCGCGG CCGC^GG
MluNI 2638 TGGCCA TGG^CCA
MscI 2638 TGGCCA TGG^CCA
NarI 991 GGCGCC GG^CGCC
NspI 3602 ACATGT [GA]CATG^[CT]
PaeR7I 3499 CTCGAG C^TCGAG
PshBI 5938 ATTAAT AT^TAAT
Psp1406I 4836 AACGTT AA^CGTT
PvuI 2381 CGATCG CGAT^CG
RcaI 4969 TCATGA T^CATGA
SacI 74 GAGCTC GAGCT^C
SacII 3634 CCGCGG CCGC^GG
ScaI 3129 AGTACT AGT^ACT
SfoI 991 GGCGCC GGC^GCC
SgrAI 1312 CACCGGTG C[GA]^CCGG[CT]G
SmaI 3090 CCCGGG CCC^GGG
SpeI 3530 ACTAGT A^CTAGT
XhoI 3499 CTCGAG C^TCGAG
XmaCI 3090 CCCGGG C^CCGGG
XmaI 3090 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1532 GTCTAC GT^[AC][GT]AC
5418 GTAGAC
AgeI 1313 ACCGGT A^CCGGT
3916 ACCGGT
Alw44I 754 GTGCAC G^TGCAC
3885 GTGCAC
AocI 30 CCTGAGG CC^T[ACGT]AGG
1598 CCTGAGG
ApaLI 754 GTGCAC G^TGCAC
3885 GTGCAC
Asp700I 3342 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5448 GAAGGACTTC
BsaWI 1313 ACCGGT [AT]^CCGG[AT]
3916 ACCGGT
BsmBI 1976 CGTCTC CGTCTC(1/5)
4577 CGTCTC
Bsp1407I 5585 TGTACA T^GTACA
5871 TGTACA
BsrGI 5585 TGTACA T^GTACA
5871 TGTACA
BstEII 1373 GGTGACC G^GT[ACGT]ACC
3863 GGTGACC
BstPI 1373 GGTGACC G^GT[ACGT]ACC
3863 GGTGACC
Bsu36I 30 CCTGAGG CC^T[ACGT]AGG
1598 CCTGAGG
DrdI 4323 GACCTTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5471 GACCTTGGGGTC
Eco81I 30 CCTGAGG CC^T[ACGT]AGG
1598 CCTGAGG
EcoO65I 1373 GGTGACC G^GT[ACGT]ACC
3863 GGTGACC
EcoRI 1226 GAATTC G^AATTC
4428 GAATTC
HgaI 2394 GACGC GACGC(5/10)
5539 GCGTC
NaeI 876 GCCGGC GCC^GGC
2921 GCCGGC
NgoMIV 876 GCCGGC G^CCGGC
2921 GCCGGC
PinAI 1313 ACCGGT A^CCGGT
3916 ACCGGT
SapI 5601 GAAGAGC GCTCTTC(1/4)
1548 GCTCTTC
SbfI 2619 CCTGCAGG CCTGCA^GG
4805 CCTGCAGG
Sse8387I 2619 CCTGCAGG CCTGCA^GG
4805 CCTGCAGG
SspBI 5585 TGTACA T^GTACA
5871 TGTACA
XmnI 3342 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5448 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AspHI AspI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflFI PflMI
PleI PpuMI PshAI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII