##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AflII       
AgeI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        AspHI       
AviII       BbeI        BciVI       BfrI        BglI        
BlpI        Bpu1102I    BsaI        BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BspDI       BspEI       
BsrBI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraIII      DrdI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRV       
EcoT22I     FseI        FspI        KasI        KspI        
MfeI        MluI        MroI        MspA1I      MunI        
MvnI        NaeI        NarI        NgoMIV      NotI        
NruI        NsiI        PacI        PinAI       PmeI        
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SbfI        SfiI        SfoI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I        345  GGTACC               G^GTACC
AcyI          184  GGCGTC               G[GA]^CG[CT]C
Asp718I       345  GGTACC               G^GTACC
AspI          201  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        5141  GGATCC               G^GATCC
BanI          345  GGTACC               G^G[CT][GA]CC
BbrPI        2333  CACGTG               CAC^GTG
BbvCI        1294  CCTCAGC              CCTCAGC(-5/-2)
BcgI         2297  GCAAAAAAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         5217  TGATCA               T^GATCA
BsaAI        2333  CACGTG               [CT]AC^GT[GA]
BsaHI         184  GGCGTC               G[GA]^CG[CT]C
BsaWI        3438  ACCGGA               [AT]^CCGG[AT]
BseRI        1292  CTCCTC               GAGGAG(10/8)
BsgI          109  CTGCAC               GTGCAG(16/14)
BsmI         4024  GAATGC               GAATGC(1/-1)
BspMI        1966  GCAGGT               ACCTGC(4/8)
BstAPI       2332  GCACGTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1332  TTCGAA               TT^CGAA
EcoNI        4737  CCTTGTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         5217  TGATCA               T^GATCA
HaeII        2204  AGCGCC               [GA]GCGC^[CT]
HgaI          185  GCGTC                GACGC(5/10)
Hin1I         184  GGCGTC               G[GA]^CG[CT]C
HpaI         2808  GTTAAC               GTT^AAC
KpnI          345  GGTACC               GGTAC^C
NcoI         4474  CCATGG               C^CATGG
NdeI         2152  CATATG               CA^TATG
NheI         2067  GCTAGC               G^CTAGC
NspV         1332  TTCGAA               TT^CGAA
PflFI         201  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        3453  CCATAAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        2333  CACGTG               CAC^GTG
PmlI         2333  CACGTG               CAC^GTG
PshAI         231  GACGAGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI          168  GTCGAC               G^TCGAC
SfuI         1332  TTCGAA               TT^CGAA
SmaI         2567  CCCGGG               CCC^GGG
StuI         3821  AGGCCT               AGG^CCT
Tth111I       201  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       3453  CCATAAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        2567  CCCGGG               C^CCGGG
XmaI         2567  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccI          168  GTCGAC               GT^[AC][GT]AC
             5272  GTCTAC              
AflIII        843  ACATGT               A^C[GA][CT]GT
             3640  ACATGT              
AhdI         1229  GACCATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3105  GACAAAAAGTC         
AlwNI        2915  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             4542  CAGAACCTG           
Asp700I      1279  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2623  GAAATACTTC          
AspEI        1229  GACCATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3105  GACAAAAAGTC         
AvrII        1219  CCTAGG               C^CTAGG
             5145  CCTAGG              
BalI           50  TGGCCA               TGG^CCA
             4712  TGGCCA              
BcnI         2567  CCCGG                CC^[GC]GG
             2568  CCGGG               
BglII        2503  AGATCT               A^GATCT
             3654  AGATCT              
BlnI         1219  CCTAGG               C^CTAGG
             5145  CCTAGG              
BsaBI        3943  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             5212  GATGTTGATC          
BspLU11I      843  ACATGT               A^CATGT
             3640  ACATGT              
CfoI         2205  GCGC                 GCG^C
             2295  GCGC                
EaeI           50  TGGCCA               [CT]^GGCC[GA]
             4712  TGGCCA              
Eam1105I     1229  GACCATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3105  GACAAAAAGTC         
HapII        2568  CCGG                 C^CGG
             3439  CCGG                
HhaI         2205  GCGC                 GCG^C
             2295  GCGC                
HinP1I       2205  GCGC                 G^CGC
             2295  GCGC                
HindIII      4334  AAGCTT               A^AGCTT
             5063  AAGCTT              
HpaII        2568  CCGG                 C^CGG
             3439  CCGG                
MaeII         427  ACGT                 A^CGT
             2334  ACGT                
MamI         3943  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             5212  GATGTTGATC          
MluNI          50  TGGCCA               TGG^CCA
             4712  TGGCCA              
MscI           50  TGGCCA               TGG^CCA
             4712  TGGCCA              
MspI         2568  CCGG                 C^CGG
             3439  CCGG                
NciI         2567  CCCGG                CC^[GC]GG
             2568  CCGGG               
SapI         2407  GAAGAGC              GCTCTTC(1/4)
             2891  GAAGAGC             
XcmI         2887  CCAAGAAGAGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5244  CCAAGTATAACCTGG     
XmnI         1279  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2623  GAAATACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaI        AvaII       BanII       BbsI        
BbvI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    Bsp1407I    BspHI       BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EarI        EcoO109I    EcoRI       
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HincII      HindII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PaeR7I      PleI        PpuMI       PstI        
RcaI        RsaI        Sau3AI      Sau96I      ScaI        
ScrFI       SexAI       SfaNI       SfcI        SmlI        
SspBI       SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoI        XhoII