################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII AccII AccIII AclI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspHI AviII BbeI BciVI BfrI BglI BlpI Bpu1102I BsaI BseAI BsiEI BsiHKAI BsiWI BsmBI BspDI BspEI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI KasI KspI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NgoMIV NotI NruI NsiI PacI PinAI PmeI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SbfI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 345 GGTACC G^GTACC AcyI 184 GGCGTC G[GA]^CG[CT]C Asp718I 345 GGTACC G^GTACC AspI 201 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 5141 GGATCC G^GATCC BanI 345 GGTACC G^G[CT][GA]CC BbrPI 2333 CACGTG CAC^GTG BbvCI 1294 CCTCAGC CCTCAGC(-5/-2) BcgI 2297 GCAAAAAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 5217 TGATCA T^GATCA BsaAI 2333 CACGTG [CT]AC^GT[GA] BsaHI 184 GGCGTC G[GA]^CG[CT]C BsaWI 3438 ACCGGA [AT]^CCGG[AT] BseRI 1292 CTCCTC GAGGAG(10/8) BsgI 109 CTGCAC GTGCAG(16/14) BsmI 4024 GAATGC GAATGC(1/-1) BspMI 1966 GCAGGT ACCTGC(4/8) BstAPI 2332 GCACGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1332 TTCGAA TT^CGAA EcoNI 4737 CCTTGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 5217 TGATCA T^GATCA HaeII 2204 AGCGCC [GA]GCGC^[CT] HgaI 185 GCGTC GACGC(5/10) Hin1I 184 GGCGTC G[GA]^CG[CT]C HpaI 2808 GTTAAC GTT^AAC KpnI 345 GGTACC GGTAC^C NcoI 4474 CCATGG C^CATGG NdeI 2152 CATATG CA^TATG NheI 2067 GCTAGC G^CTAGC NspV 1332 TTCGAA TT^CGAA PflFI 201 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3453 CCATAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2333 CACGTG CAC^GTG PmlI 2333 CACGTG CAC^GTG PshAI 231 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SalI 168 GTCGAC G^TCGAC SfuI 1332 TTCGAA TT^CGAA SmaI 2567 CCCGGG CCC^GGG StuI 3821 AGGCCT AGG^CCT Tth111I 201 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3453 CCATAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 2567 CCCGGG C^CCGGG XmaI 2567 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 168 GTCGAC GT^[AC][GT]AC 5272 GTCTAC AflIII 843 ACATGT A^C[GA][CT]GT 3640 ACATGT AhdI 1229 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3105 GACAAAAAGTC AlwNI 2915 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG 4542 CAGAACCTG Asp700I 1279 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2623 GAAATACTTC AspEI 1229 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3105 GACAAAAAGTC AvrII 1219 CCTAGG C^CTAGG 5145 CCTAGG BalI 50 TGGCCA TGG^CCA 4712 TGGCCA BcnI 2567 CCCGG CC^[GC]GG 2568 CCGGG BglII 2503 AGATCT A^GATCT 3654 AGATCT BlnI 1219 CCTAGG C^CTAGG 5145 CCTAGG BsaBI 3943 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5212 GATGTTGATC BspLU11I 843 ACATGT A^CATGT 3640 ACATGT CfoI 2205 GCGC GCG^C 2295 GCGC EaeI 50 TGGCCA [CT]^GGCC[GA] 4712 TGGCCA Eam1105I 1229 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3105 GACAAAAAGTC HapII 2568 CCGG C^CGG 3439 CCGG HhaI 2205 GCGC GCG^C 2295 GCGC HinP1I 2205 GCGC G^CGC 2295 GCGC HindIII 4334 AAGCTT A^AGCTT 5063 AAGCTT HpaII 2568 CCGG C^CGG 3439 CCGG MaeII 427 ACGT A^CGT 2334 ACGT MamI 3943 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5212 GATGTTGATC MluNI 50 TGGCCA TGG^CCA 4712 TGGCCA MscI 50 TGGCCA TGG^CCA 4712 TGGCCA MspI 2568 CCGG C^CGG 3439 CCGG NciI 2567 CCCGG CC^[GC]GG 2568 CCGGG SapI 2407 GAAGAGC GCTCTTC(1/4) 2891 GAAGAGC XcmI 2887 CCAAGAAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5244 CCAAGTATAACCTGG XmnI 1279 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2623 GAAATACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaI AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PaeR7I PleI PpuMI PstI RcaI RsaI Sau3AI Sau96I ScaI ScrFI SexAI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoI XhoII