##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
Acc65I AclI AgeI AscI Asp700I
Asp718I AvrII BclI BlnI BsaBI
BspDI BspLU11I BssHII Bst1107I BstBI
BstZ17I ClaI CpoI EcoRI FbaI
FseI HindIII HpaI KpnI MamI
MfeI MluI MunI NdeI NotI
NruI NspV PacI PaeR7I PinAI
PmeI Psp1406I RsrII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 5353 GACGTC GACGT^C
AccIII 270 TCCGGA T^CCGGA
AhdI 587 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 5460 ATTAAT AT^TAAT
AsnI 5460 ATTAAT AT^TAAT
AspEI 587 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2659 TGCGCA TGC^GCA
BamHI 2268 GGATCC G^GATCC
BciVI 3650 GGATAC GTATCC(6/5)
BseAI 270 TCCGGA T^CCGGA
BsiWI 30 CGTACG C^GTACG
BsmBI 4062 CGTCTC CGTCTC(1/5)
Bsp1407I 4077 TGTACA T^GTACA
BspEI 270 TCCGGA T^CCGGA
BsrGI 4077 TGTACA T^GTACA
DraIII 1459 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 609 CGGCCG C^GGCCG
Eam1105I 587 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 609 CGGCCG C^GGCCG
Eco52I 609 CGGCCG C^GGCCG
EcoRV 464 GATATC GAT^ATC
EcoT22I 323 ATGCAT ATGCA^T
FspI 2659 TGCGCA TGC^GCA
HincII 2875 GTTGAC GT[CT]^[GA]AC
HindII 2875 GTTGAC GT[CT]^[GA]AC
KspI 925 CCGCGG CCGC^GG
MroI 270 TCCGGA T^CCGGA
NaeI 449 GCCGGC GCC^GGC
NcoI 2402 CCATGG C^CATGG
NgoMIV 449 GCCGGC G^CCGGC
NsiI 323 ATGCAT ATGCA^T
PflMI 1159 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 5185 GACTACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5460 ATTAAT AT^TAAT
PvuI 2119 CGATCG CGAT^CG
SacII 925 CCGCGG CCGC^GG
SapI 2721 GCTCTTC GCTCTTC(1/4)
ScaI 218 AGTACT AGT^ACT
SspBI 4077 TGTACA T^GTACA
SspI 5443 AATATT AAT^ATT
Van91I 1159 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 506 GTAGAC GT^[AC][GT]AC
822 GTCTAC
AflII 4321 CTTAAG C^TTAAG
4675 CTTAAG
AflIII 2901 ACGTGT A^C[GA][CT]GT
3563 ACGTGT
AocI 2263 CCTCAGG CC^T[ACGT]AGG
3471 CCTTAGG
Aor51HI 1433 AGCGCT AGC^GCT
2129 AGCGCT
ApaI 2236 GGGCCC GGGCC^C
4757 GGGCCC
BbeI 782 GGCGCC GGCGC^C
1301 GGCGCC
BfrI 4321 CTTAAG C^TTAAG
4675 CTTAAG
BglI 1116 GCCCCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1930 GCCGTGTTGGC
BglII 4704 AGATCT A^GATCT
5237 AGATCT
BsaWI 270 TCCGGA [AT]^CCGG[AT]
4021 TCCGGT
BsiEI 609 CGGCCG CG[GA][CT]^CG
2119 CGATCG
BstEII 2196 GGTGACC G^GT[ACGT]ACC
4479 GGTGACC
BstPI 2196 GGTGACC G^GT[ACGT]ACC
4479 GGTGACC
Bsu36I 2263 CCTCAGG CC^T[ACGT]AGG
3471 CCTTAGG
DraI 3740 TTTAAA TTT^AAA
5438 TTTAAA
DrdI 2878 GACACTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3899 GACTTGCCGGTC
EarI 1469 CTCTTC CTCTTC(1/4)
2722 CTCTTC
Eco47III 1433 AGCGCT AGC^GCT
2129 AGCGCT
Eco81I 2263 CCTCAGG CC^T[ACGT]AGG
3471 CCTTAGG
EcoO65I 2196 GGTGACC G^GT[ACGT]ACC
4479 GGTGACC
KasI 782 GGCGCC G^GCGCC
1301 GGCGCC
Ksp632I 1469 CTCTTC CTCTTC(1/4)
2722 CTCTTC
MslI 2312 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5274 CATCCAAGTG
NarI 782 GGCGCC GG^CGCC
1301 GGCGCC
NheI 1542 GCTAGC G^CTAGC
3274 GCTAGC
SfoI 782 GGCGCC GGC^GCC
1301 GGCGCC
SmlI 4321 CTTAAG C^T[CT][GA]AG
4675 CTTAAG
SphI 4001 GCATGC GCATG^C
4202 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AspI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvCI BbvI BcgI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PleI PmaCI PmlI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XhoII
XmaCI XmaI