##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AviII BamHI BbeI BbrPI BciVI
BfrI BglI BsaAI BsaWI BseAI
BsiWI BspDI BspEI BspLU11I BsrFI
BssHII Bst1107I BstBI BstXI BstZ17I
Cfr10I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoT22I FseI FspI
HaeII KasI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NsiI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 3144 GTCTAC GT^[AC][GT]AC
AcyI 4155 GGCGTC G[GA]^CG[CT]C
AflIII 2939 ACACGT A^C[GA][CT]GT
AhdI 3718 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3718 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2704 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5191 CCTAGG C^CTAGG
BcgI 1191 GCACCGAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4719 TGATCA T^GATCA
BlnI 5191 CCTAGG C^CTAGG
BlpI 342 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 342 GCTCAGC GC^T[ACGT]AGC
BsaBI 812 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4155 GGCGTC G[GA]^CG[CT]C
BsaI 2335 GAGACC GGTCTC(1/5)
BsiEI 14 CGACCG CG[GA][CT]^CG
BsmBI 4157 CGTCTC CGTCTC(1/5)
BspMI 3234 GCAGGT ACCTGC(4/8)
BsrBI 271 CCGCTC CCGCTC(-3/-3)
BssSI 1544 CTCGTG CACGAG(-5/-1)
CelII 342 GCTCAGC GC^T[ACGT]AGC
DraII 4335 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1018 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2187 GACTACAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3718 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 4335 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 5678 GATATC GAT^ATC
FbaI 4719 TGATCA T^GATCA
Hin1I 4155 GGCGTC G[GA]^CG[CT]C
HindIII 1668 AAGCTT A^AGCTT
KspI 237 CCGCGG CCGC^GG
MamI 812 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NruI 385 TCGCGA TCG^CGA
NspI 4529 ACATGC [GA]CATG^[CT]
PaeR7I 183 CTCGAG C^TCGAG
PflFI 2704 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 4335 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 3336 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1196 GAGCTC GAGCT^C
SacII 237 CCGCGG CCGC^GG
StuI 1352 AGGCCT AGG^CCT
Tth111I 2704 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 183 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 1435 GATCC GGATC(4/5)
2203 GGATC
AocI 760 CCTGAGG CC^T[ACGT]AGG
3949 CCTCAGG
AseI 5173 ATTAAT AT^TAAT
5813 ATTAAT
AsnI 5173 ATTAAT AT^TAAT
5813 ATTAAT
AvaI 183 CTCGAG C^[CT]CG[GA]G
293 CTCGGG
BbsI 3507 GTCTTC GAAGAC(2/6)
5978 GTCTTC
BbvCI 644 CCTCAGC CCTCAGC(-5/-2)
29 GCTGAGG
BpuAI 3507 GTCTTC GAAGAC(2/6)
5978 GTCTTC
BsgI 2798 GTGCAG GTGCAG(16/14)
5474 GTGCAG
BsoBI 183 CTCGAG C^[CT]CG[GA]G
293 CTCGGG
Bsp1407I 743 TGTACA T^GTACA
1173 TGTACA
BsrGI 743 TGTACA T^GTACA
1173 TGTACA
Bsu36I 760 CCTGAGG CC^T[ACGT]AGG
3949 CCTCAGG
CfoI 347 GCGC GCG^C
5196 GCGC
Eco81I 760 CCTGAGG CC^T[ACGT]AGG
3949 CCTCAGG
HgaI 213 GCGTC GACGC(5/10)
4156 GCGTC
HhaI 347 GCGC GCG^C
5196 GCGC
HinP1I 347 GCGC G^CGC
5196 GCGC
MaeII 2941 ACGT A^CGT
5211 ACGT
PshBI 5173 ATTAAT AT^TAAT
5813 ATTAAT
SspBI 743 TGTACA T^GTACA
1173 TGTACA
SspI 2074 AATATT AAT^ATT
2596 AATATT
XbaI 436 TCTAGA T^CTAGA
2470 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AluI AlwNI ApoI Asp700I Asp718I
AspHI AvaII BalI BanI BanII
BbvI BcnI BfaI BglII BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BsrDI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DsaI EaeI EarI EcoNI
EcoO65I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PstI
PvuII RcaI RsaI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI