##################################
# NO CUT ENZYMES (   50 enzymes) #
##################################

AclI        AflII       AgeI        AscI        AvrII       
BbeI        BbvCI       BclI        BfrI        BlnI        
BsiWI       BspDI       BspHI       BssHII      Bst1107I    
BstZ17I     ClaI        CpoI        DrdI        EagI        
EclXI       Eco52I      EcoT22I     FbaI        FseI        
HpaI        KasI        MfeI        MunI        NarI        
NheI        NotI        NruI        NsiI        PacI        
PinAI       PmeI        PshAI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SapI        SfiI        
SfoI        SgrAI       SnaBI       StuI        SwaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII        3377  GACGTC               GACGT^C
AccIII       1011  TCCGGA               T^CCGGA
AcyI         3377  GACGTC               G[GA]^CG[CT]C
AhdI         3715  GACTGAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       3314  GTGCAC               G^TGCAC
AocI         2329  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       891  AGCGCT               AGC^GCT
ApaLI        3314  GTGCAC               G^TGCAC
AseI         4459  ATTAAT               AT^TAAT
AsnI         4459  ATTAAT               AT^TAAT
AspEI        3715  GACTGAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        4148  TGCGCA               TGC^GCA
BcgI         2790  GCACCTCCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1939  GGATAC               GTATCC(6/5)
BsaBI        1297  GATCGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        3377  GACGTC               G[GA]^CG[CT]C
BseAI        1011  TCCGGA               T^CCGGA
BspEI        1011  TCCGGA               T^CCGGA
BsrBI        2875  GAGCGG               CCGCTC(-3/-3)
Bsu36I       2329  CCTGAGG              CC^T[ACGT]AGG
DraI         5781  TTTAAA               TTT^AAA
Eam1105I     3715  GACTGAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      891  AGCGCT               AGC^GCT
Eco81I       2329  CCTGAGG              CC^T[ACGT]AGG
EcoRI        1379  GAATTC               G^AATTC
EcoRV        3487  GATATC               GAT^ATC
FspI         4148  TGCGCA               TGC^GCA
HgaI         3431  GCGTC                GACGC(5/10)
Hin1I        3377  GACGTC               G[GA]^CG[CT]C
HincII       1519  GTCAAC               GT[CT]^[GA]AC
HindII       1519  GTCAAC               GT[CT]^[GA]AC
KspI         4064  CCGCGG               CCGC^GG
MamI         1297  GATCGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         3429  ACGCGT               A^CGCGT
MroI         1011  TCCGGA               T^CCGGA
NaeI         3446  GCCGGC               GCC^GGC
NdeI         5327  CATATG               CA^TATG
NgoMIV       3446  GCCGGC               G^CCGGC
PaeR7I        753  CTCGAG               C^TCGAG
PshBI        4459  ATTAAT               AT^TAAT
SacII        4064  CCGCGG               CCGC^GG
SbfI         2160  CCTGCAGG             CCTGCA^GG
ScaI         1893  AGTACT               AGT^ACT
SexAI        4993  ACCTGGT              A^CC[AT]GGT
SpeI         5229  ACTAGT               A^CTAGT
SphI         3288  GCATGC               GCATG^C
Sse8387I     2160  CCTGCAGG             CCTGCA^GG
SspI         5288  AATATT               AAT^ATT
XhoI          753  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI         2246  GTAGAC               GT^[AC][GT]AC
             3575  GTCTAC              
ApaI         4068  GGGCCC               GGGCC^C
             4350  GGGCCC              
AspI          683  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1586  GACATCGTC           
BalI         1872  TGGCCA               TGG^CCA
             2469  TGGCCA              
BbrPI        1514  CACGTG               CAC^GTG
             2045  CACGTG              
BglII        1903  AGATCT               A^GATCT
             2451  AGATCT              
BsiEI        2734  CGACCG               CG[GA][CT]^CG
             2800  CGACCG              
BsmBI        3379  CGTCTC               CGTCTC(1/5)
             1355  GAGACG              
BsmI          429  GCATTC               GAATGC(1/-1)
             1206  GCATTC              
BspLU11I     3808  ACATGT               A^CATGT
             4703  ACATGT              
BsrFI        2736  ACCGGC               [GA]^CCGG[CT]
             3446  GCCGGC              
BstBI         357  TTCGAA               TT^CGAA
              797  TTCGAA              
BstEII       4809  GGTAACC              G^GT[ACGT]ACC
             4989  GGTGACC             
BstPI        4809  GGTAACC              G^GT[ACGT]ACC
             4989  GGTGACC             
BstXI        1606  CCAGGAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3036  CCAAAGTCCTGG        
Cfr10I       2736  ACCGGC               [GA]^CCGG[CT]
             3446  GCCGGC              
EcoO65I      4809  GGTAACC              G^GT[ACGT]ACC
             4989  GGTGACC             
HaeII         891  AGCGCT               [GA]GCGC^[CT]
             4828  AGCGCC              
MluNI        1872  TGGCCA               TGG^CCA
             2469  TGGCCA              
MscI         1872  TGGCCA               TGG^CCA
             2469  TGGCCA              
NcoI          504  CCATGG               C^CATGG
             2988  CCATGG              
NspV          357  TTCGAA               TT^CGAA
              797  TTCGAA              
PflFI         683  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1586  GACATCGTC           
PmaCI        1514  CACGTG               CAC^GTG
             2045  CACGTG              
PmlI         1514  CACGTG               CAC^GTG
             2045  CACGTG              
SfuI          357  TTCGAA               TT^CGAA
              797  TTCGAA              
Tth111I       683  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1586  GACATCGTC           
XbaI         2705  TCTAGA               T^CTAGA
             4285  TCTAGA              
XcmI          639  CCAAAGAGAACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              961  CCAAAACTACTTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  130 enzymes) #
#########################################

Acc65I      AccII       AciI        AcsI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApoI        
Asp700I     Asp718I     AspHI       AvaI        AvaII       
BamHI       BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    Bsp1407I    BspMI       BsrDI       BsrGI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       BstYI       Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
KpnI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
SspBI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       XmaCI       XmaI        XmnI