##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BamHI BbeI BbrPI
BclI BlnI BsaWI BseAI BsiWI
BspEI BspHI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I CpoI DraIII
DrdI Eam1105I Eco47III EcoNI FbaI
FseI KasI KpnI KspI MfeI
MluI MroI MunI NarI NdeI
NheI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 915 GACGTC GACGT^C
AccII 369 CGCG CG^CG
AcyI 915 GACGTC G[GA]^CG[CT]C
AocI 4414 CCTTAGG CC^T[ACGT]AGG
AspI 3788 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3991 TGCGCA TGC^GCA
BalI 3492 TGGCCA TGG^CCA
BglI 1342 GCCAGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 205 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 205 GCTGAGC GC^T[ACGT]AGC
BsaAI 3178 TACGTG [CT]AC^GT[GA]
BsaBI 4445 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 915 GACGTC G[GA]^CG[CT]C
BsiEI 2 CGGCCG CG[GA][CT]^CG
BsmBI 1287 GAGACG CGTCTC(1/5)
BsmI 1167 GAATGC GAATGC(1/-1)
BspDI 1599 ATCGAT AT^CGAT
BsrDI 4420 GCAATG GCAATG(2/0)
BstAPI 792 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 444 GGTGACC G^GT[ACGT]ACC
BstPI 444 GGTGACC G^GT[ACGT]ACC
BstUI 369 CGCG CG^CG
Bsu36I 4414 CCTTAGG CC^T[ACGT]AGG
CelII 205 GCTGAGC GC^T[ACGT]AGC
ClaI 1599 ATCGAT AT^CGAT
EagI 2 CGGCCG C^GGCCG
EclXI 2 CGGCCG C^GGCCG
Eco52I 2 CGGCCG C^GGCCG
Eco81I 4414 CCTTAGG CC^T[ACGT]AGG
EcoO65I 444 GGTGACC G^GT[ACGT]ACC
EcoRI 4565 GAATTC G^AATTC
EcoRV 960 GATATC GAT^ATC
FspI 3991 TGCGCA TGC^GCA
HgaI 45 GCGTC GACGC(5/10)
Hin1I 915 GACGTC G[GA]^CG[CT]C
HindIII 519 AAGCTT A^AGCTT
HpaI 3046 GTTAAC GTT^AAC
MamI 4445 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3492 TGGCCA TGG^CCA
MscI 3492 TGGCCA TGG^CCA
MvnI 369 CGCG CG^CG
NaeI 11 GCCGGC GCC^GGC
NgoMIV 11 GCCGGC G^CCGGC
NotI 1 GCGGCCGC GC^GGCCGC
PflFI 3788 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4006 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 656 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 705 GAGCTC GAGCT^C
ScaI 1414 AGTACT AGT^ACT
SphI 1549 GCATGC GCATG^C
StuI 2187 AGGCCT AGG^CCT
Tth111I 3788 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4006 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BanI 309 GGCACC G^G[CT][GA]CC
2414 GGCACC
BbsI 747 GAAGAC GAAGAC(2/6)
2399 GTCTTC
BcgI 1517 CGAGAAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3839 CGATCCTCCTGC
BciVI 1753 GGATAC GTATCC(6/5)
2878 GGATAC
BpuAI 747 GAAGAC GAAGAC(2/6)
2399 GTCTTC
BsgI 1642 CTGCAC GTGCAG(16/14)
3273 CTGCAC
Bsp1407I 265 TGTACA T^GTACA
3165 TGTACA
BsrBI 237 CCGCTC CCGCTC(-3/-3)
2499 CCGCTC
BsrGI 265 TGTACA T^GTACA
3165 TGTACA
PpuMI 114 GGGTCCC [GA]G^G[AT]CC[CT]
760 AGGTCCT
SmaI 472 CCCGGG CCC^GGG
1041 CCCGGG
SpeI 1562 ACTAGT A^CTAGT
3050 ACTAGT
SspBI 265 TGTACA T^GTACA
3165 TGTACA
SspI 3009 AATATT AAT^ATT
4066 AATATT
XbaI 2782 TCTAGA T^CTAGA
3036 TCTAGA
XmaCI 472 CCCGGG C^CCGGG
1041 CCCGGG
XmaI 472 CCCGGG C^CCGGG
1041 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanII BbvCI BbvI
BcnI BfaI BfrI BglII BmyI
BpmI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII