##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

AatII       AclI        AflII       Alw44I      ApaLI       
AscI        AspI        AviII       AvrII       BbsI        
BcgI        BciVI       BfrI        BlnI        BpuAI       
BsaBI       BsiEI       BspDI       BspHI       BssHII      
BssSI       BstBI       ClaI        CpoI        DraIII      
DrdI        EagI        EclXI       Eco52I      FseI        
FspI        HindIII     MamI        MfeI        MluI        
MunI        NaeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PflFI       PmeI        
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SbfI        SexAI       SfiI        SfuI        
SgrAI       Sse8387I    SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I       1836  GGTACC               G^GTACC
AccIII       3601  TCCGGA               T^CCGGA
AgeI         1138  ACCGGT               A^CCGGT
AocI         1831  CCTGAGG              CC^T[ACGT]AGG
AseI         2236  ATTAAT               AT^TAAT
AsnI         2236  ATTAAT               AT^TAAT
Asp700I      5574  GAATGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1836  GGTACC               G^GTACC
BamHI         160  GGATCC               G^GATCC
BbrPI        1306  CACGTG               CAC^GTG
BclI         3321  TGATCA               T^GATCA
BglI         4752  GCCTTAATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1905  AGATCT               A^GATCT
BsaI          422  GAGACC               GGTCTC(1/5)
BseAI        3601  TCCGGA               T^CCGGA
BsiWI        1323  CGTACG               C^GTACG
BsmBI        2600  GAGACG               CGTCTC(1/5)
Bsp1407I     4676  TGTACA               T^GTACA
BspEI        3601  TCCGGA               T^CCGGA
BsrBI        4442  GAGCGG               CCGCTC(-3/-3)
BsrGI        4676  TGTACA               T^GTACA
BstEII       4535  GGTTACC              G^GT[ACGT]ACC
BstPI        4535  GGTTACC              G^GT[ACGT]ACC
Bsu36I       1831  CCTGAGG              CC^T[ACGT]AGG
Eco81I       1831  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      4535  GGTTACC              G^GT[ACGT]ACC
EcoRV        1589  GATATC               GAT^ATC
EcoT22I      3372  ATGCAT               ATGCA^T
FbaI         3321  TGATCA               T^GATCA
HpaI         3985  GTTAAC               GTT^AAC
KpnI         1836  GGTACC               GGTAC^C
KspI          657  CCGCGG               CCGC^GG
MroI         3601  TCCGGA               T^CCGGA
NcoI         4732  CCATGG               C^CATGG
NdeI         3375  CATATG               CA^TATG
NsiI         3372  ATGCAT               ATGCA^T
PinAI        1138  ACCGGT               A^CCGGT
PmaCI        1306  CACGTG               CAC^GTG
PmlI         1306  CACGTG               CAC^GTG
PpuMI        2027  GGGACCC              [GA]G^G[AT]CC[CT]
PshBI        2236  ATTAAT               AT^TAAT
SacII         657  CCGCGG               CCGC^GG
SapI         1764  GCTCTTC              GCTCTTC(1/4)
SnaBI        4856  TACGTA               TAC^GTA
SpeI          293  ACTAGT               A^CTAGT
SspBI        4676  TGTACA               T^GTACA
SspI         1127  AATATT               AAT^ATT
StuI          390  AGGCCT               AGG^CCT
XbaI          104  TCTAGA               T^CTAGA
XmnI         5574  GAATGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   42 enzymes) #
###################################

AccII         658  CGCG                 CG^CG
              862  CGCG                
AcyI          546  GGCGCC               G[GA]^CG[CT]C
              722  GGCGCC              
AflIII       1184  ACATGT               A^C[GA][CT]GT
             4673  ACATGT              
AhdI         2978  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3392  GACCAGAAGTC         
AlwNI        3458  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             4946  CAGGGTCTG           
Aor51HI       577  AGCGCT               AGC^GCT
             4709  AGCGCT              
ApaI         1223  GGGCCC               GGGCC^C
             1362  GGGCCC              
AspEI        2978  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3392  GACCAGAAGTC         
BbeI          546  GGCGCC               GGCGC^C
              722  GGCGCC              
BlpI         3722  GCTAAGC              GC^T[ACGT]AGC
             4439  GCTGAGC             
Bpu1102I     3722  GCTAAGC              GC^T[ACGT]AGC
             4439  GCTGAGC             
BsaHI         546  GGCGCC               G[GA]^CG[CT]C
              722  GGCGCC              
BsaWI        1138  ACCGGT               [AT]^CCGG[AT]
             3601  TCCGGA              
BsgI         1345  CTGCAC               GTGCAG(16/14)
             4502  CTGCAC              
BspLU11I     1184  ACATGT               A^CATGT
             4673  ACATGT              
BsrFI        1138  ACCGGT               [GA]^CCGG[CT]
             1996  ACCGGC              
Bst1107I     3860  GTATAC               GTA^TAC
             4859  GTATAC              
BstAPI        766  GCACGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1436  GCAAGGTCTGC         
BstUI         658  CGCG                 CG^CG
              862  CGCG                
BstYI         160  GGATCC               [GA]^GATC[CT]
             1905  AGATCT              
BstZ17I      3860  GTATAC               GTA^TAC
             4859  GTATAC              
CelII        3722  GCTAAGC              GC^T[ACGT]AGC
             4439  GCTGAGC             
Cfr10I       1138  ACCGGT               [GA]^CCGG[CT]
             1996  ACCGGC              
DsaI          657  CCGCGG               C^C[GA][CT]GG
             4732  CCATGG              
Eam1105I     2978  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3392  GACCAGAAGTC         
Eco47III      577  AGCGCT               AGC^GCT
             4709  AGCGCT              
Hin1I         546  GGCGCC               G[GA]^CG[CT]C
              722  GGCGCC              
KasI          546  GGCGCC               G^GCGCC
              722  GGCGCC              
MflI          160  GGATCC               [GA]^GATC[CT]
             1905  AGATCT              
MvnI          658  CGCG                 CG^CG
              862  CGCG                
NarI          546  GGCGCC               GG^CGCC
              722  GGCGCC              
PaeR7I       2827  CTCGAG               C^TCGAG
             3814  CTCGAG              
PflMI        3457  CCAGAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5514  CCATTGCCTGG         
SacI          110  GAGCTC               GAGCT^C
             3518  GAGCTC              
SfoI          546  GGCGCC               GGC^GCC
              722  GGCGCC              
SmaI         1456  CCCGGG               CCC^GGG
             4549  CCCGGG              
SphI         4162  GCATGC               GCATG^C
             4387  GCATGC              
Van91I       3457  CCAGAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5514  CCATTGCCTGG         
XhoI         2827  CTCGAG               C^TCGAG
             3814  CTCGAG              
XhoII         160  GGATCC               [GA]^GATC[CT]
             1905  AGATCT              
XmaCI        1456  CCCGGG               C^CCGGG
             4549  CCCGGG              
XmaI         1456  CCCGGG               C^CCGGG
             4549  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbvCI       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaAI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspMI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstXI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       EaeI        
EarI        EcoNI       EcoO109I    EcoRI       EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScaI        ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI