##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccI AclI AgeI AhdI
Alw44I ApaLI AspEI AspI AvaI
AviII BbrPI BbvCI BcgI BclI
BglI BsiEI BsiWI BsoBI BspDI
BsrBI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
FbaI FseI FspI KspI MluI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI Sse8387I
SwaI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 447 GGTACC G^GTACC
AccII 3053 CGCG CG^CG
Aor51HI 1653 AGCGCT AGC^GCT
ApaI 2003 GGGCCC GGGCC^C
AscI 3051 GGCGCGCC GG^CGCGCC
AseI 4452 ATTAAT AT^TAAT
AsnI 4452 ATTAAT AT^TAAT
Asp700I 2216 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 447 GGTACC G^GTACC
AvrII 1844 CCTAGG C^CTAGG
BamHI 471 GGATCC G^GATCC
BbeI 84 GGCGCC GGCGC^C
BciVI 392 GGATAC GTATCC(6/5)
BlnI 1844 CCTAGG C^CTAGG
BlpI 2492 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2492 GCTCAGC GC^T[ACGT]AGC
BsaAI 1565 CACGTA [CT]AC^GT[GA]
BspHI 3556 TCATGA T^CATGA
BsrFI 381 GCCGGC [GA]^CCGG[CT]
BssHII 3052 GCGCGC G^CGCGC
BstUI 3053 CGCG CG^CG
BstXI 2289 CCACAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2492 GCTCAGC GC^T[ACGT]AGC
Cfr10I 381 GCCGGC [GA]^CCGG[CT]
DraIII 2919 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1653 AGCGCT AGC^GCT
EcoNI 2330 CCTAAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 5325 GAATTC G^AATTC
HpaI 1457 GTTAAC GTT^AAC
KasI 84 GGCGCC G^GCGCC
KpnI 447 GGTACC GGTAC^C
MvnI 3053 CGCG CG^CG
NaeI 381 GCCGGC GCC^GGC
NarI 84 GGCGCC GG^CGCC
NcoI 720 CCATGG C^CATGG
NdeI 3586 CATATG CA^TATG
NgoMIV 381 GCCGGC G^CCGGC
NheI 2455 GCTAGC G^CTAGC
PflMI 2261 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 4452 ATTAAT AT^TAAT
RcaI 3556 TCATGA T^CATGA
SacI 2714 GAGCTC GAGCT^C
ScaI 5400 AGTACT AGT^ACT
SfoI 84 GGCGCC GGC^GCC
SpeI 5311 ACTAGT A^CTAGT
StuI 1937 AGGCCT AGG^CCT
Van91I 2261 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2216 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 474 TCCGGA T^CCGGA
4696 TCCGGA
AlwNI 48 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3976 CAGGTGCTG
AocI 2971 CCTCAGG CC^T[ACGT]AGG
4409 CCTTAGG
BalI 978 TGGCCA TGG^CCA
2779 TGGCCA
BanI 84 GGCGCC G^G[CT][GA]CC
447 GGTACC
BanII 2003 GGGCCC G[GA]GC[CT]^C
2714 GAGCTC
BglII 1519 AGATCT A^GATCT
1779 AGATCT
BsaBI 1613 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5080 GATCTTGATC
BsaWI 474 TCCGGA [AT]^CCGG[AT]
4696 TCCGGA
BseAI 474 TCCGGA T^CCGGA
4696 TCCGGA
BsgI 312 CTGCAC GTGCAG(16/14)
2549 GTGCAG
BsmBI 2559 GAGACG CGTCTC(1/5)
2618 GAGACG
BspEI 474 TCCGGA T^CCGGA
4696 TCCGGA
BsrDI 3852 CATTGC GCAATG(2/0)
4359 GCAATG
BstAPI 2114 GCAAAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3847 GCATCCATTGC
Bsu36I 2971 CCTCAGG CC^T[ACGT]AGG
4409 CCTTAGG
EaeI 978 TGGCCA [CT]^GGCC[GA]
2779 TGGCCA
Eco81I 2971 CCTCAGG CC^T[ACGT]AGG
4409 CCTTAGG
EcoRV 622 GATATC GAT^ATC
682 GATATC
EcoT22I 401 ATGCAT ATGCA^T
3648 ATGCAT
HaeII 84 GGCGCC [GA]GCGC^[CT]
1653 AGCGCT
HgaI 2561 GACGC GACGC(5/10)
2620 GACGC
HindIII 1421 AAGCTT A^AGCTT
3658 AAGCTT
MaeII 1560 ACGT A^CGT
1566 ACGT
MamI 1613 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5080 GATCTTGATC
MluNI 978 TGGCCA TGG^CCA
2779 TGGCCA
MroI 474 TCCGGA T^CCGGA
4696 TCCGGA
MscI 978 TGGCCA TGG^CCA
2779 TGGCCA
NsiI 401 ATGCAT ATGCA^T
3648 ATGCAT
PpuMI 2367 GGGACCT [GA]G^G[AT]CC[CT]
2421 AGGACCT
PstI 161 CTGCAG CTGCA^G
1293 CTGCAG
SphI 399 GCATGC GCATG^C
3646 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AflIII AluI AlwI ApoI AspHI
AvaII BbsI BbvI BcnI BfaI
BfrI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspLU11I BspMI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MspI MunI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII