##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AflII       
Aor51HI     AscI        AseI        AsnI        Asp718I     
AviII       AvrII       BalI        BbrPI       BcgI        
BfrI        BlnI        BsaAI       BsaBI       BseAI       
BsiEI       BsiWI       BspDI       BspEI       BsrBI       
BssHII      BstEII      BstPI       BstUI       ClaI        
CpoI        DrdI        EagI        EclXI       Eco47III    
Eco52I      EcoO65I     EcoT22I     FseI        FspI        
KpnI        KspI        MamI        MluI        MluNI       
MroI        MscI        MvnI        NaeI        NdeI        
NgoMIV      NotI        NruI        NsiI        PacI        
PflMI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SgrAI       SnaBI       
Sse8387I    StuI        SwaI        Van91I      


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AatII        2490  GACGTC               GACGT^C
AgeI         2166  ACCGGT               A^CCGGT
AhdI         2891  GACCACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      1307  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        2891  GACCACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI          208  GGCGCC               G^G[CT][GA]CC
BbeI          208  GGCGCC               GGCGC^C
BsmBI        2492  CGTCTC               CGTCTC(1/5)
BspHI        4385  TCATGA               T^CATGA
BspLU11I     4864  ACATGT               A^CATGT
BspMI        4467  ACCTGC               ACCTGC(4/8)
BsrFI        2166  ACCGGT               [GA]^CCGG[CT]
BssSI        1266  CTCGTG               CACGAG(-5/-1)
Bst1107I     6001  GTATAC               GTA^TAC
BstAPI       4948  GCACTAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      6001  GTATAC               GTA^TAC
Cfr10I       2166  ACCGGT               [GA]^CCGG[CT]
DraIII       4212  CACACGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     2891  GACCACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         662  CCTGGCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1603  GAATTC               G^AATTC
HgaI         5019  GACGC                GACGC(5/10)
HpaI         4694  GTTAAC               GTT^AAC
KasI          208  GGCGCC               G^GCGCC
NarI          208  GGCGCC               GG^CGCC
NheI         2561  GCTAGC               G^CTAGC
PinAI        2166  ACCGGT               A^CCGGT
RcaI         4385  TCATGA               T^CATGA
ScaI         6112  AGTACT               AGT^ACT
SfiI          665  GGCCGAGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          208  GGCGCC               GGC^GCC
SphI         5375  GCATGC               GCATG^C
SspI         4814  AATATT               AAT^ATT
XmnI         1307  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI         5142  GTCTAC               GT^[AC][GT]AC
             6001  GTATAC              
AcyI          208  GGCGCC               G[GA]^CG[CT]C
             2490  GACGTC              
AflIII       4864  ACATGT               A^C[GA][CT]GT
             5599  ACGTGT              
ApaI         1160  GGGCCC               GGGCC^C
             3877  GGGCCC              
AspI          100  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3242  GACCTAGTC           
BamHI         923  GGATCC               G^GATCC
             2435  GGATCC              
BbvCI        2080  CCTCAGC              CCTCAGC(-5/-2)
             2000  GCTGAGG             
BciVI        2627  GGATAC               GTATCC(6/5)
              931  GTATCC              
BglII         320  AGATCT               A^GATCT
             1663  AGATCT              
BsaHI         208  GGCGCC               G[GA]^CG[CT]C
             2490  GACGTC              
BsaWI        2124  ACCGGA               [AT]^CCGG[AT]
             2166  ACCGGT              
BseRI        2236  CTCCTC               GAGGAG(10/8)
              669  GAGGAG              
BsmFI        3333  GTCCC                GGGAC(10/14)
             4131  GTCCC               
BstBI         957  TTCGAA               TT^CGAA
             3192  TTCGAA              
EcoRV        3023  GATATC               GAT^ATC
             3186  GATATC              
FauI          630  CCCGC                CCCGC(4/6)
              522  GCGGG               
Hin1I         208  GGCGCC               G[GA]^CG[CT]C
             2490  GACGTC              
MfeI         2871  CAATTG               C^AATTG
             3043  CAATTG              
MunI         2871  CAATTG               C^AATTG
             3043  CAATTG              
NspV          957  TTCGAA               TT^CGAA
             3192  TTCGAA              
PaeR7I        778  CTCGAG               C^TCGAG
             3694  CTCGAG              
PflFI         100  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3242  GACCTAGTC           
PpuMI        4030  AGGACCC              [GA]G^G[AT]CC[CT]
             5703  AGGTCCT             
PstI         1092  CTGCAG               CTGCA^G
             2018  CTGCAG              
SfuI          957  TTCGAA               TT^CGAA
             3192  TTCGAA              
SmaI          251  CCCGGG               CCC^GGG
             1157  CCCGGG              
SpeI         5839  ACTAGT               A^CTAGT
             5978  ACTAGT              
Tth111I       100  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3242  GACCTAGTC           
XhoI          778  CTCGAG               C^TCGAG
             3694  CTCGAG              
XmaCI         251  CCCGGG               C^CCGGG
             1157  CCCGGG              
XmaI          251  CCCGGG               C^CCGGG
             1157  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        Alw44I      
AlwI        AlwNI       AocI        ApaLI       ApoI        
AspHI       AvaI        AvaII       BanII       BbsI        
BbvI        BclI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaI        BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BsrDI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
FbaI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PvuII       RsaI        SacI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XbaI        XcmI        XhoII